Nucleotide Sequence of the <i>Nicotiana tabacum</i> cv Xanthi Gene Encoding 1-Aminocyclopropane-1-Carboxylate Synthase

Bailey, Bryan A.; Avni, Adi; Li, Ning; Mattoo, Autar K.; Anderson, James D.

doi:10.1104/pp.100.3.1615

Cited by 32 publications

(16 citation statements)

References 10 publications

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“…Using the GAP program in the GCG software package for the alignment of NT-ACS2 with ACS genes recently isolated in our laboratory and those deposited in the Genbank, we found that this newly identified ACS gene has 67·2% identity in amino acid sequence and 65% identity in nucleotide sequence, respectively, to that of the previously published NT-ACS1 (Bailey et al 1993). The ACC synthase coded for by NT-ACS2 gene has, respectively, 89·9, 90·7, 82·1, 73·1, 77·1, 71, 72·3, 63·1 and 44% identity in polypeptide sequence to ACC synthases from tomato (LE-ACS1A and LE-ACS1B) apple (MD-ACS2), Citrus (CS-ACS1), Pelargonium hortorum (PH-ACS2), Helianthus annuus (HA-ACS1), carnation (DC-ACS2) and wheat (TA-ACS2 and TA-ACS1).…”

Section: Phylogenetic Analysis Of Nt-acs2 and Other Acs Genesmentioning

confidence: 86%

“…Comparative alignment of the five cDNA fragments revealed that they shared 51-83% identity ( Table 1). One of them was identical to NT-ACS1, the previously reported tobacco ACC synthase gene (Bailey et al 1993). The other four newly discovered ACC synthase genes were arbitrarily named as NT-ACS2, NT-ACS3, NT-ACS4 and NT-ACS5.…”

Section: Isolation Of Acc Synthase Cdna Clones From Tobacco Plantmentioning

confidence: 90%

“…We identified four new tobacco ACC synthase genes in addition to the previously isolated tobacco ACS gene (NT-ACS1) responsive to ethyleneinducing xylanase and Cu 2+ (Bailey et al 1993;Avni et al 1994). One of them, NT-ACS2, was indeed found to be multiple environmental stress-responsive (MESR) as we hoped to find.…”

Section: Introductionmentioning

confidence: 85%

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Identification of a novel multiple environmental factor‐responsive 1‐aminocyclopropane‐1‐carboxylate synthase gene, NT‐ACS2, from tobacco

Liu²,

Wong

et al. 2000

Plant Cell & Environment

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Many abiotic environmental factors elicit the production of stress‐ethylene in higher plants. To elucidate the molecular mechanisms underlying the regulation of stress‐ethylene production and the physiological roles played by stress‐ethylene in stress responses of plants, we studied the gene expression of ACC synthase in tobacco plants that had been subjected to environmental stresses. Four new tobacco ACC synthase cDNA fragments, NT‐ACS2, NT‐ACS3, NT‐ACS4 and NT‐ACS5, were identified and sequenced. It was found that NT‐ACS2 could be induced by wounding, cold temperature and, especially, sunlight. NT‐ACS4 was induced at a faster kinetics by wounding. The multiple environmental stress‐responsive (MESR) NT‐ACS2 gene was found to contain three introns and four exons and encode a polypeptide of 484 amino acids, 54·6 kDa and pI 6·87. Computer analysis of the 3·4 kb 5′ flanking region upstream of the ACS coding region revealed the existence of a group of putative cis‐acting regulatory elements potentially conferring wounding, chilling, and UV light inducibility. Phylogenetic analysis of ACC synthase genes of different plant origins indicated that the chill‐inducible NT‐ACS2 gene is closely related to a chilling‐inducible citrus ACS gene.

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Section: Phylogenetic Analysis Of Nt-acs2 and Other Acs Genesmentioning

confidence: 86%

Section: Isolation Of Acc Synthase Cdna Clones From Tobacco Plantmentioning

confidence: 90%

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Identification of a novel multiple environmental factor‐responsive 1‐aminocyclopropane‐1‐carboxylate synthase gene, NT‐ACS2, from tobacco

Liu²,

Wong

et al. 2000

Plant Cell & Environment

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“…The ACC oxidase gene was a 604-bp fragment that was PCR-amplified from a Xanthi cDNA library [34]. The synthase gene was also obtained from a Xanthi cDNA library and was cloned into plasmid pTACC-13 [35]. Probes were prepared using [ 32 P]dCTP (Amersham) and the Oligolabelling Kit from Pharmacia Biotech.…”

Section: Rna Isolation and Northern Analysismentioning

confidence: 99%

Induction of defense responses in tobacco by the protein Nep1 from Fusarium oxysporum

et al. 2001

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“…Yip et al, 1992), winter squash (Nakajima et al, 1990), zucchini (Huang et al, 1991), Arabidopsis (Liang et al, 1992; Van Der Straeten et al, 1992), mung bean (Botellaet al, 1992; Kim et al, 1992), carnation (Park et al, 1992), orchid (ONeill et al, 1993), tobacco (Bailey et al, 1992), and apple (Dong et ai., 1991; Kim et al, 1992). In several cases, it has been shown that ACC synthase genes belong to a multigene family and that they are differentially responsive to various ethyleneinducing factors and conditions such as wounding, fruit ripening, and auxins, although in certain cases some leve1 of coordination was demonstrated (Nakajima et al, 1990; Van Der Straeten et al, 1990; Dong et al, 1991;Olson et al, 1991; Rottmann et al, 1991; Yip et ai., 1992).…”

Section: Introductionmentioning

confidence: 99%

The Arabidopsis 1-Aminocyclopropane-1-Carboxylate Synthase Gene 1 Is Expressed during Early Development.

et al. 1993

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The temporal and spatlal expression of one member of the Arabidopsis 1-aminocyclopropane-1-carboxylate (ACC) synthase gene family (ACS7) was analyzed using a promoter-P-glucuronidase fusion. The expression of ACSl is under developmental control both in shoot and root. High expression was observed in young tissues and was switched off in mature tissues. ACS7 pmmoter activity was strongly correlated with lateral root formation. Dark-grown seedlings exhibited a different expression pattern from light-grown ones. The ACC content and the in vivo activity of ACC oxidase were determined. ACC content correlated with ACS7 gene activity. ACC oxidase activity was demonstrated in young Arabidopsis seedlings. Thus, the ACC formed can be converted into ethylene. In addition, ethylene production of immature leaves was fourfold higher compared to that of mature leaves. The possible involvement of ACSl in influencing plant growth and development is discussed. INTRODUCTIONEthylene is involved in several aspects of plant development from germination and seedling growth to flowering, fruit ripening, leaf abscission, and organ senescence. It also plays a key role in the response to environmental factors (Abeles, 1973; Yang and Hoffman, 1984; Moore, 1989; Van Der Straeten and Van Montagu, 1991).With the recent cloning of genes encoding the two most important enzymes in the biosynthesis of ethylene, l-aminocyclopropane-1-carboxylate (ACC) synthase (Nakajima et al., 1990; Van Der Straeten et al., 1990; Dong et al., 1991;Huang et al., 1991; Olson et al., 1991; and ACC oxidase (ethylene-forming enzyme or EFE) (Hamilton et al., 1990; Spanu et al., 1991;Wang and Woodson, 1991), more information about the molecular regulation of the synthesis of this hormone has been made available. The main focus has been on the regulation of ACC synthase gene expression because of its key regulatory role in the pathway. This implies that diverse inducers of ethylene production are also inducers of de novo synthesis of ACC synthase (Yang and Hoffmann, 1984; Van Der Straeten and Van Montagu, 1991).ACC synthases have been cloned from tomato (Van Der Straeten et al., 1990; Olson et al., 1991; To whom correspondence should be addressed.Yip et al., 1992), winter squash (Nakajima et al., 1990), zucchini (Huang et al., 1991), Arabidopsis (Liang et al., 1992; Van Der Straeten et al., 1992), mung bean (Botellaet al., 1992; Kim et al., 1992), carnation (Park et al., 1992), orchid (ONeill et al., 1993), tobacco (Bailey et al., 1992), and apple (Dong et ai., 1991; Kim et al., 1992). In several cases, it has been shown that ACC synthase genes belong to a multigene family and that they are differentially responsive to various ethyleneinducing factors and conditions such as wounding, fruit ripening, and auxins, although in certain cases some leve1 of coordination was demonstrated (Nakajima et al., 1990; Van Der Straeten et al., 1990; Dong et al., 1991; Olson et al., 1991; ; Yip et ai., 1992). These studies have provided information about general aspects of AC...

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Nucleotide Sequence of the Nicotiana tabacum cv Xanthi Gene Encoding 1-Aminocyclopropane-1-Carboxylate Synthase

Cited by 32 publications

References 10 publications

Identification of a novel multiple environmental factor‐responsive 1‐aminocyclopropane‐1‐carboxylate synthase gene, NT‐ACS2, from tobacco

Identification of a novel multiple environmental factor‐responsive 1‐aminocyclopropane‐1‐carboxylate synthase gene, NT‐ACS2, from tobacco

Induction of defense responses in tobacco by the protein Nep1 from Fusarium oxysporum

The Arabidopsis 1-Aminocyclopropane-1-Carboxylate Synthase Gene 1 Is Expressed during Early Development.

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