Occurrence of actinomycetes in indoor air in Rio de Janeiro, Brazil

Grigorevski-Lima, A. L.; Silva-Filho, Renato G.; Coelho, R. R. R.

doi:10.1016/j.buildenv.2005.06.009

Cited by 11 publications

(7 citation statements)

References 21 publications

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“…However, the most frequently identified actinomycetes were Streptomyces that accounted from 16 to 29% of all bacterial isolates in the air of S6. In addition, Grigorevski-Lima et al [8] found six different species of Streptomyces as prevalent inhabitants of variety indoor environments, including hospitals. Among these actinomycetes and Nocardia, which are commonly detected on house dust, garden soils and pools, some strains pathogenic to humans have been reported [59,60].…”

Section: Resultsmentioning

confidence: 99%

“…Moreover, indoor air of a hospital was colonised by population of actinomycetes that ranged from 0 to 11.7 cfu m -3 air that corresponded to 1.5% of total microbial colonies counted. In addition, 88% of these bacteria, mostly represented by potential pathogenic strains of Streptomyces, were found in the three final stages of the Andersen sampler that simulate the human respiratory tract [8]. The major source of airborne bacteria is the presence of humans whose activities like talking, coughing, sneezing, walking and washing can release different biological particles [38].…”

Section: Introductionmentioning

confidence: 99%

“…Background concentration of actinomycetes measuring in the residential air is below 10 2 cfu m -3 air; however, the concentration may be even several hundred times higher in some sites (eg nearby a composting site) [5][6][7]. Grigorevski-Lima et al [8] detected actinomycetes in 35 out of 53 collecting points from nine different indoor environments. Although these bacteria were present in many sampling point, their frequency was very low compared with fungal and total bacterial counts, representing only 1.5% of these microorganisms.…”

Section: Introductionmentioning

confidence: 99%

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Assessment of Airborne Actinomycetes in Subterranean and Earth Sanatoriums / Występowanie Promieniowców W Pomieszczeniu Sanatoriów Podziemnym Oraz Naziemnym

Frączek¹,

Kozdrój²

2013

Ecological Chemistry and Engineering S

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Actinomycetes are common soil microorganisms; however, they have also been detected in air samples, becoming important air contaminants in occupational and residential habitats. Concentrations of airborne actinomycetes and their distribution among different fractions of bioaerosol were determined in selected sites of two sanatorium types, ie a subterranean located in the former salt-mine of Bochnia and standard earth spa of Szczawnica. The average concentrations of actinomycetes ranged from 0 to almost 40 cfu m -3, for the sanatorium of Bochnia, and from 7 to 125 cfu m -3 , for Szczawnica spa, throughout the year. The respiratory fraction of the bioaerosol ranged from 25 to 51% and from 78 to 81% in the subterranean sites and rooms of Szczawnica spa, respectively. For both sanatoriums, among isolated actinomycetes dominated species belonging to Rhodococcus, Streptomyces and Nocardia. The results indicate that patients can be differentially exposed to airborne actinomycetes in compared sanatoriums. The air of underground spa reveals to be of better quality, regarding the bacteria characteristics.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Assessment of Airborne Actinomycetes in Subterranean and Earth Sanatoriums / Występowanie Promieniowców W Pomieszczeniu Sanatoriów Podziemnym Oraz Naziemnym

Frączek¹,

Kozdrój²

2013

Ecological Chemistry and Engineering S

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“…The few studies related to actinomycetes in indoor environments are very specific, such as toxin production by a single isolated strain, or studies concerning pathogeny (Andersson et al 1998;Grigorevski-Limaa et al 2006). In the present study the numbers of airborne microorganisms, particularly actinomycetes, in the indoor environment of the church of Saint Katherine were investigated.…”

Section: Carbon Utilization and Acid Production From Different Carbonmentioning

confidence: 99%

Characterization of actinomycetes isolated from the indoor air of the church of Saint Katherine Monastery, Egypt

Abdulla¹,

Morshedy²,

Dewedar³

2007

Aerobiologia

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Actinomycetes were isolated from the indoor air of the church of Saint Katherin Monastery during different visiting hours. Fifty air samples collected over one year by using automated air sampler were plated on four different media. The low nutrient-content medium 1/10 SC was significantly effective in recovery of actinomycetes compared to the other formula of rich media. Average counts of bacteria, actinomycetes and fungi were 487, 65 and 90 cfu/m 3 , respectively. Fifty-six morphologically different actinomycetes isolates were recovered during this study assigned into five different genera, in addition to three unidentified isolates. Tentative identification of the isolates indicated predominance of genus Streptomyces, representing 59% of the isolates. Isolates were screened for resistance to 11 antibiotics, antimicrobial activities towards seven microbial strains, Growth on 12 different carbon source, acid production and pigmentation. About 77% of actinomycete isolates were resistant to the antibiotics with different resistance patterns. 12.5% of the airborne actinomycetes exhibited antinmicrobial activities. The isolates showed wide variation in carbon source usage. Forty percent of the isolates were able to utilize all tested carbon sources while 80% were acid producers. Melanin production was produced by 34% of the isolates. While 45% of the isolates were able to produce other diffusible pigments, the majority of the pigments were brown in color however; yellow, orange and green pigments were produced by a number of isolates. The impact of these activities on the historical objects of the church and the tourist's health is discussed.

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“…Nos estabelecimentos que processam e manipulam alimentos, a contaminação alimentar e ambiental está fortemente atrelada com a formação de aerossóis, que servem como carreadores de micro-organismos, os quais podem ser provenientes tanto do trato respiratório, da pele, de mucosas e de cabelos dos manipuladores, quanto do ambiente (GRIGOREVSKI-LIMA et al, 2006;DOORES et al, 2015). Para Venter et al (2004) e a APA (2009), a contaminação aérea é importante do ponto de vista sanitário e econômico, haja vista que os micro-organismos, que permanecem no ar, são mais resistentes a fatores ambientais limitantes, como o dessecamento, quando comparado à microbiota não carreada por esse meio.…”

Section: Introductionunclassified

Monitoramento da Qualidade Microbiológica Ambiental em Unidade de Alimentação

Martins¹,

Braga²

2019

UNIC

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Restaurantes podem propiciar doenças veiculadas por alimentos, assim, medidas de controle devem ser realizadas. Objetivou-se quantificar coliformes totais, termotolerantes e bactérias heterotróficas mesófilas na superfície da bancada da pia de manipulação, e bioaerossóis fúngicos em unidade de alimentação em uma instituição de ensino, antes e durante o processamento dos alimentos, e após higienização do local de trabalho. As amostras foram colhidas por swab e o ar, pela exposição, durante 15 minutos, de placas de PDA acidificado, em diferentes dias da semana totalizando dezesseis coletas. Coliformes totais (35 ºC, 24/48 h) e termotolerantes (45 ºC, 24 h) foram quantificados por inoculação de diluições seriadas, em caldo VB e EC, respectivamente, e os resultados expressos em NMP/cm2. A contagem de mesófilos (35 °C, 48 h) foi realizada em ágar PCA, e os resultados expressos em UFC/cm2. Os fungos anemófilos cultivados a 25 ºC por sete dias foram expressos em UFC/15 min. Os resultados foram analisados em software ASSISTAT 7.6 betas. Foi verificada contagem > 103NMP/cm2 para coliformes totais durante o processamento, e após higienização, decaimento de 16% em relação à média inicial. Redução de 56,3% foi observada para coliformes termotolerantes. O número de mesófilos se mantiveram ≥ 3 ciclos log, independentemente, do período da coleta (antes, durante ou após processamento). 54,2% das amostras do ar apresentaram altas contagens para fungos (> 50 UFC/15 min.). Os resultados indicam a possível geração de riscos e ocorrência de toxinfecções alimentares. Quando bem realizada, a higienização é eficaz na redução da carga microbiana. Sugere-se melhor monitoramento dos procedimentos realizados pelos manipuladores. Palavras-chave: Aerossóis. Coliformes. Mesófilos. AbstractRestaurants can provide food-borne diseases, so control measures must be taken. The objective of this study was to quantify total coliforms, thermotolerant bacteria and mesophilic heterotrophic bacteria on the sink basin surface, and fungal bioaerosols in a feeding unit at a teaching institution, before and during food processing, and after cleaning the workplace. Samples were collected by swab and air by exposure for 15 minutes of acidified PDA plates on different days of the week totaling sixteen samples. Total (35°C, 24/48 h) and thermotolerant coliforms (45 °C, 24 h) were quantified by inoculation of serial dilutions in VB and EC broth, respectively, and the results expressed as MLN/cm2. The counts of mesophiles (35 °C, 48 h) were performed on PCA agar and the results expressed in CFU/cm2. Anemophilous fungi cultured at 25°C for seven days were expressed in CFU/15 min. The results were analyzed in ASSISTAT 7.6 betas software. It was verified a count > 103 MLN/cm2 for total coliforms during the processing, and after sanitization, decay of 16% in relation to the initial average. Reduction of 56.3% was observed for thermotolerant coliforms. The number of mesophiles remained ≥ 3 log cycles, regardless of the collection period (before, during or after processing). 54.2% of the air samples had high counts for fungi (> 50 CFU/15 min). The results indicate the possible generation of risks and occurrence of alimentary toxinfections. When properly performed, hygiene is effective in reducing microbial load. It is suggested better procedures monitoring performed by the handlers. Keywords: Aerosols. Coliforms. Mesophiles.

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Occurrence of actinomycetes in indoor air in Rio de Janeiro, Brazil

Cited by 11 publications

References 21 publications

Assessment of Airborne Actinomycetes in Subterranean and Earth Sanatoriums / Występowanie Promieniowców W Pomieszczeniu Sanatoriów Podziemnym Oraz Naziemnym

Assessment of Airborne Actinomycetes in Subterranean and Earth Sanatoriums / Występowanie Promieniowców W Pomieszczeniu Sanatoriów Podziemnym Oraz Naziemnym

Characterization of actinomycetes isolated from the indoor air of the church of Saint Katherine Monastery, Egypt

Monitoramento da Qualidade Microbiológica Ambiental em Unidade de Alimentação

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