Occurrence of Yersinia enterocolitica in Poultry Products

Abstract

In a study on isolation of Yersinia enterocolitica from poultry products, usefulness of the following enrichment media was evaluated: phosphate-buffered saline solution with and without addition of 1% sorbitol plus 0.15% bile salts, modified Rappaport medium and selenite broth. Plating was performed on MacConkey agar directly from the incubated broths and after treatment of the enrichment broths with a potassium hydroxide solution. Seventy-three of 108 (68%) samples of poultry products contained Y. enterocolitica. Y. enterocolitica was isolated most frequently after enrichment in phosphate-buffered saline solution with sorbitol and bile salts. Nearly all Y. enterocolitica strains were found after KOH-treatment. Serotyping showed that the isolated strains mainly belonged to the category of the non-pathogenic, so called “environmental” strains.

Bacteriological status of fish marketed in Beni-Suef City

“…Yersinia enterocolitica is a psychrotrophic organism which was associated with a variety of human illnesses; of which enterocolitis is the most frequent manifestation. The organism has become of considerable public health significance, many outbreaks of food borne infection caused by consumption of contaminated food by the organism, this substantiate the hypothesis reported by (De-Boer et al, 1986). The presence of Yersinia enterocolitica in the examined samples could be attributed to the contamination of the water from which they caught by the organism, and might from the contaminated sewage sludge, this held the view reported by (Langeland, 1983).…”

A novel semi-selective medium for Pseudomonas protegens isolation from soil samples

“…At a concentration of 10 μg/mL, this antimicrobial agent inhibited the growth of a diverse range of environmental bacteria from outside the family Pseudomonadaceae. Irgasan® is typically used in selective media for P. aeruginosa (Fonseca et al, 1986;Lilly and Lowbury, 1972), but it is also included in CIN agar (Cefsulodin Irgasan® Novobiocin) for the specific isolation of Yersinia from foods or clinical samples (De Boer et al, 1982).…”