On-line conversion and determination of artemisinin and its kinetic parameters using orthogonal design by coupling of flow injection with capillary electrophoresis

A rapid method for the determination of artemisinin and its biosynthetic precursors in Artemisia annua L. crude extracts

“…An earlier method involved conversion of (1) to a UV absorbing compound Q260 to facilitate its detection [17][18][19]. The disadvantage of this method is that the derivatization procedure from (1) to Q260 is time consuming and introduces significant experimental errors [20]. Other methods include gas chromatography with flame ionization (GC-FID) [21,22], supercritical fluid chromatography with ELSD [23], FID [24] and MS [25] detection.…”

Preparation of Bupleurum Nasal Spray and Evaluation on Its Safety and Efficacy

“…14,15) As solubilizing agents, TW-80 (in 10% (w/v) solution), PG and TC were all examined at 4 levels. The essential oil, extracted from 4.5 kg Radix Bupleuri, was divided into 16 parts, and according to the L 16 (4 5 ) orthogonal table (Table 1), TW-80, PG and/or TC were added to each tube before water was filled until the final volume was 18.75 ml (1 ml solution containing oil from 15 g Bupleuri), followed by stirring for 5 min.…”

Combination of flow injection with electrophoresis using capillaries and chips

“…Therefore, efficient automation of these operations is highly desired for future development. A CE coupled with FI method for the determination of artemisinin and its relevant kinetic parameters by on-line treatment with alkali has been developed by Cheng et al [25]. Orthogonal experimental design has been utilized to optimize experimental factors that affect the analysis of artemisinin.…”