On-line Vapor-Phase Generation Followed by Fourier-Transform Infrared Spectrometry for the Quantitative Analysis of Water-Soluble Penicillin G in Pharmaceutical Formulations

Abstract

The combination of vapor-phase generation (VPG) and Fourier-transform infrared (FTIR) spectrometry was performed as an alternative analytical technique for the determination of water-soluble penicillin G (PENG). Samples were transferred into a heated reactor, and a potassium iodate solution was injected into the reactor. Carbon monoxide (CO) generated under these conditions was carried via a N2 gas carrier stream inside the IR gas cell, and the corresponding FTIR spectra were continuously recorded as a function of time. Analytical measurements were made using the maximum absorbance of the CO band at 2170 ± 4 cm -1 . Various factors influencing the analytical signals were evaluated and selected. The figures of merit of the proposed method involve a linear calibration curve over the range of 3 to 320 mg L -1 , a limit of detection of 0.5 mg L -1 and a precision of 2.6%. The method was successfully applied to PENG determination in pharmaceutical preparations.

Direct chemiluminescence determination of penicillin G potassium and a chemometrical optimization approach

“…Various analytical methods for the determination of PGK in pharmaceutical formulations have been proposed (3,4). They include spectrophotometry-solid phase extraction with UV detection at 230 nm (3), Fourier transform infrared spectrometry (5) and chemiluminescence (4) methods. Chemiluminescence is becoming a powerful analytical tool with widespread applications in various fields, owing to its high sensitivity, wide dynamic range and low-cost instrumentation (6)(7)(8)(9).…”

Untitled

“…In order to take advantage of spectroscopic techniques, chemometric approaches such as Partial Least Squares (PLS) regression have frequently been used in quantitative spectral analysis to obtain very selective information from unselective data. Since two decades, tremendous quantitative works, applied efficiently on quantification of pharmaceutical formulations, have been published 11,12 .…”