Open‐tubular capillary electrochromatography using carboxylatopillar[5]arene as stationary phase

Abstract

Pillar[n]arenes have achieved much interest in material chemistry and supramolecular chemistry due to unusual pillar shape structure and high selectivity toward guest. However, pillar[n]arenes have not yet been applied in capillary electrochromatography. This work at first time reports that carboxylatopillar[5]arene is used as a stationary phase in open-tubular capillary electrochromatography. Carboxylatopillar[5]arene not only possess the advantages of pillar[n]arenes but also provide free carboxy groups for immobilizing on the inner wall of capillary column via covalent bonding. The characterization of SEM and FT-IR indicated that carboxylatopillar[5]arene was successfully grafted on the inner wall of capillary. The baseline separation of model analytes including neutral, basic, and acidic compounds, nonsteroidal anti-inflammatory drugs and dansyl-amino acids have been achieved thanks to the electron-rich cavity of carboxylatopillar[5]arene and hydrophobic interactions between the analytes and stationary phase. The intraday, interday, and column-to-column precisions (RSDs) of retention time and peak area for the neutral analytes were all less than 3.34 and 9.65%, respectively. This work indicates that pillar[n]arenes have great potential in capillary electrochromatography as novel stationary phase.

Recent applications and chiral separation development based on stationary phases in open tubular capillary electrochromatography (2019–2022)

“…And it deserves notice that stimuli-responsive polymers [ 150 ] are a kind of potential material for protein isomerism and glycoisoform separation. Non-traditional materials [ 151 , 152 ] have also been used in OT column coatings for achiral separation. Some of these coatings partially solve the previous limitations of OT columns.…”

Recent developments of monolithic and open‐tubular capillary electrochromatography (2017–2019)

“…In recent years, there were many novel homo‐polymers or copolymers synthesized in order to meet the needs for the separation of different analytes . Liu and co‐workers reported an example of partially filled PLOT capillary column with immobilized pH gradient (PLOT‐IPG) for OT‐CEC analysis of amino acids and proteins.…”

Interaksi Molekuler Amonium Hidroksida

“…Antara NH4 + dan OHberikan ion (115,118,(130)(131)(132)(133)(134)(135) . Sedangkan atom-atom dalam NH4OH berikatan kovalen (136)(137)(138)(139)(140)(141)(142)(143)(144)(145) .Ketika NH4 + direaksikan dengan OH -, maka senyawa NH4 + akan menarik elektron dari OH -. Dengan demikian, akibat adanya perbedaan muatan dari ion-ion NH4OH mengakibatkan ion-ion tersebut tarik-menarik sehinggak membentuk senyawa NH4OH.…”