Ophthalmic dosage forms for drug delivery to posterior segment

Lee, Jaemin; Rhee, Yun-Seok

doi:10.1007/s40005-021-00554-8

Cited by 12 publications

(11 citation statements)

References 96 publications

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“…DEXA/MP was formulated using an oil-in-water emulsion. , Briefly, 4 mg of DEXA (Sigma-Aldrich, Saint Louis, MO, USA) in 0.2 mL methanol and 76 mg of PLGA (Resomer RG504 H; Sigma-Aldrich) in 0.8 mL dichloromethane (DCM; Junsei Chemical, Tokyo, Japan) were mixed to form a clear organic phase solution. Next, the mixture was added to 5 mL of 1% poly(vinyl alcohol) solution to homogenize at 21,000 rpm for 4 min.…”

Section: Methodsmentioning

confidence: 99%

Scalable and Uniform Fabrication of Dexamethasone-Eluting Depot-Engineered Stem Cell Spheroids as a Microtissue Construct to Target Bone Regeneration

Nguyen

Phuong

Shrestha

et al. 2023

ACS Appl. Mater. Interfaces

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Potentiation of stem cell potency is critical for successful tissue engineering, especially for bone regeneration. Three-dimensional cell culture and bioactive molecule co-delivery with cells have been proposed to achieve this effect. Here, we provide a uniform and scalable fabrication of osteogenic microtissue constructs of mesenchymal stem cell (MSC) spheroids surfaceengineered with dexamethasone-releasing polydopamine-coated microparticles (PD-DEXA/MPs) to target bone regeneration. The microparticle conjugation process was rapid and cell-friendly and did not affect the cell viability or key functionalities. The incorporation of DEXA in the conjugated system significantly enhanced the osteogenic differentiation of MSC spheroids, as evidenced by upregulating osteogenic gene expression and intense alkaline phosphatase and alizarin red S staining. In addition, the migration of MSCs from spheroids was tested on a biocompatible macroporous fibrin scaffold (MFS). The result showed that PD-DEXA/MPs were stably anchored on MSCs during cell migration over time. Finally, the implantation of PD-DEXA/MP-conjugated spheroid-loaded MFS into a calvarial defect in a mouse model showed substantial bone regeneration. In conclusion, the uniform fabrication of microtissue constructs containing MSC spheroids with drug depots shows a potential to improve the performance of MSCs in tissue engineering.

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Section: Methodsmentioning

confidence: 99%

Scalable and Uniform Fabrication of Dexamethasone-Eluting Depot-Engineered Stem Cell Spheroids as a Microtissue Construct to Target Bone Regeneration

Nguyen

Phuong

Shrestha

et al. 2023

ACS Appl. Mater. Interfaces

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“…Passive delivery to the intravitreal compartment is challenging due to anatomical structure, physiological barriers and unfavorable physicochemical characteristics of the APIs. [37][38][39] Direct intravitreal administration of drugs is known to cause significant increase in the intraocular pressure (IOP) and other side effects. There is an urgent need to deliver antifungal and anti-inflammatory drugs intravitreally to treat several ophthalmic disorders pertaining to the posterior segment.…”

Section: Intravitreal Delivery Using Pcp Microneedlesmentioning

confidence: 99%

Polymer Coated Polymeric (PCP) Microneedles for Controlled Delivery of Drugs (Dermal and Intravitreal)

Jakka

Matadh²,

Shankar

et al. 2022

Journal of Pharmaceutical Sciences

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“…Many conventional dosage forms (eye drops and ointments) cannot achieve therapeutic concentrations in the posterior region of eye since only an extremely small amount of drug (1/100,000) can reach the retina and choroid. Although investigations into novel dosage forms that can be applied topically are underway, the topical dosage form might be formed and may target posterior segment diseases [1,2].…”

Section: The Ophthalmic Preparationsmentioning

confidence: 99%