Optimization and characterization of extracellular cellulase produced by Bacillus pumilus MGB05 isolated from midgut of muga silkworm (Antheraea assamensis Helfer)

Bhuyan, Pinky Moni; Sandilya, Sosanka Protim; Nath, Pranab Kumar; Gandotra, Sakshi; Subramanian, S.; Kardong, Devid; Gogoi, Dhrubajyoti

doi:10.1016/j.aspen.2018.08.004

Cited by 17 publications

(6 citation statements)

References 32 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The stimulatory effect of Mg 2+ and Ca 2+ on cellulase was also re-ported by Yoon et al Bakare et al [69,70]. Fe 2+ reduced FPase activity by 33.4% [71]. The negative effect of Fe 2+ on cellulase was also reported by Yin et al [72].…”

Section: Discussionsupporting

confidence: 53%

Coconut Mesocarp-Based Lignocellulosic Waste as a Substrate for Cellulase Production from High Promising Multienzyme-Producing Bacillus amyloliquefaciens FW2 without Pretreatments

et al. 2022

View full text Add to dashboard Cite

Facing the crucial issue of high cost in cellulase production from commercial celluloses, inexpensive lignocellulosic materials from agricultural wastes have been attractive. Therefore, several studies have focused on increasing the efficiency of cellulase production by potential microorganisms capable of secreting a high and diversified amount of enzymes using agricultural waste as valuable substrates. Especially, extremophilic bacteria play an important role in biorefinery due to their high value catalytic enzymes that are active even under harsh environmental conditions. Therefore, in this study, we aim to investigate the ability to produce cellulase from coconut-mesocarp of the potential bacterial strain FW2 that was isolated from kitchen food waste in South Korea. This strain was tolerant in a wide range of temperature (–6–75 °C, pH range (4.5–12)) and at high salt concentration up to 35% NaCl. The molecular weight of the purified cellulase produced from strain FW2 was estimated to be 55 kDa. Optimal conditions for the enzyme activity using commercial substrates were found to be 40–50 °C, pH 7.0–7.5, and 0–10% NaCl observed in 920 U/mL of CMCase, 1300 U/mL of Avicelase, and 150 U/mL of FPase. It was achieved in 650 U/mL, 720 U/mL, and 140 U/mL of CMCase, Avicelase, and FPase using coconut-mesocarp, respectively. The results revealed that enzyme production by strain FW2 may have significant commercial values for industry, argo-waste treatment, and other potential applications.

show abstract

Section: Discussionsupporting

confidence: 53%

Coconut Mesocarp-Based Lignocellulosic Waste as a Substrate for Cellulase Production from High Promising Multienzyme-Producing Bacillus amyloliquefaciens FW2 without Pretreatments

et al. 2022

View full text Add to dashboard Cite

show abstract

“…Similar optimum temperature values for the activity of the studied enzymes were previously reported in other bacterial strains of the Bacillus genus [25]. Isolated from the midgut of muga silkworm, B. pumilus MGB05 achieved the highest activity of FPase at 50 °C [48]. Previously, Kazeem et al [49] noted a 20 °C higher optimum temperature for FPases produced by B. licheniformis 2D55.…”

Section: Effect Of Temperature and Ph On The Cellulases' Activitysupporting

confidence: 82%

Cellulolytic Properties of a Potentially Lignocellulose-Degrading Bacillus sp. 8E1A Strain Isolated from Bulk Soil

2022

View full text Add to dashboard Cite

Cellulolytic enzymes produced by spore-forming bacteria seem to be a potential solution to the degradation of lignocellulosic waste. In this study, several dozen bacterial spore-forming strains were isolated from soil and one of them was selected for further studies. The studied bacterial strain was identified to genus Bacillus (strain 8E1A) by 16S rRNA gene sequencing. Bacillus sp. 8E1A showed an activity of carboxymethyl cellulase (CMCase) with visualization with Congo Red-25 mm (size of clear zone). To study CMCase, filter paper hydrolase (FPase), and microcrystalline cellulose Avicel hydrolase (Avicelase) production, three different cellulose sources were used for bacterial strain cultivation: carboxymethyl cellulose (CMC), filter paper (FP), and microcrystalline cellulose Avicel. The highest CMCase (0.617 U mL−1), FPase (0.903 U mL−1), and Avicelase (0.645 U mL−1) production of Bacillus sp. 8E1A was noted for using CMC (after 216 h of incubation), Avicel cellulose (after 144 h of incubation), and CMC (after 144 h of incubation), respectively. Subsequently, the cellulases’ activity was measured at various temperatures and pH values. The optimal temperature for CMCase (0.535 U mL−1) and Avicelase (0.666 U mL−1) activity was 70 °C. However, the highest FPase (0.868 U mL−1) activity was recorded at 60 °C. The highest CMCase and Avicelase activity was recorded at pH 7.0 (0.520 and 0.507 U mL−1, respectively), and the optimum activity of FPase was noted at pH 6.0 (0.895 U mL−1). These results indicate that the cellulases produced by the Bacillus sp. 8E1A may conceivably be used for lignocellulosic waste degradation in industrial conditions.

show abstract

“…While Bacillus pumilus, Actinobacterium sp., and Bacillus licheniformis are silkworm gut associated strains. They play a vital role in silkworm health as they enhance the development and growth of larvae and reduced larval mortality (Mala and Vijila, 2018;Yeruva et al, 2019;Bhuyan et al, 2018). Bacillus pumilus has antiviral activity against silkworm nucleopolyhedrovirus in vitro (Liu et al, 2018).…”

Section: Discussionmentioning

confidence: 99%

Construction, Characterization, and Biosensing Potential of an SOS Inducible mCherry Based Reporter Plasmid

Bano

2024

PJZ

View full text Add to dashboard Cite

show abstract

Optimization and characterization of extracellular cellulase produced by Bacillus pumilus MGB05 isolated from midgut of muga silkworm (Antheraea assamensis Helfer)

Cited by 17 publications

References 32 publications

Coconut Mesocarp-Based Lignocellulosic Waste as a Substrate for Cellulase Production from High Promising Multienzyme-Producing Bacillus amyloliquefaciens FW2 without Pretreatments

Coconut Mesocarp-Based Lignocellulosic Waste as a Substrate for Cellulase Production from High Promising Multienzyme-Producing Bacillus amyloliquefaciens FW2 without Pretreatments

Cellulolytic Properties of a Potentially Lignocellulose-Degrading Bacillus sp. 8E1A Strain Isolated from Bulk Soil

Construction, Characterization, and Biosensing Potential of an SOS Inducible mCherry Based Reporter Plasmid

Contact Info

Product

Resources

About