Optimization and Validation Methods for Assay of Acyclovir Cream Determination by High Performance Liquid Chromatography

Zaenudin, Muhamad; Situmorang, Almawati; Supandi, S

doi:10.1088/1755-1315/819/1/012055

Cited by 2 publications

(2 citation statements)

References 5 publications

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“…This method was successfully adapted for use in the analysis of pharmacokinetic profiles of ACV tablets. The relevant literature on the detection of ACV by HPLC [71,72] was summarized, and the main parameters are shown in Table 7. Yet, the biggest challenge in determining ACV by HPLC is that the measured peaks are easily towed, and the retention time is relatively long.…”

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

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Critical Review of Synthesis, Toxicology and Detection of Acyclovir

Wei

Yao

et al. 2021

Molecules

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Acyclovir (ACV) is an effective and selective antiviral drug, and the study of its toxicology and the use of appropriate detection techniques to control its toxicity at safe levels are extremely important for medicine efforts and human health. This review discusses the mechanism driving ACV’s ability to inhibit viral coding, starting from its development and pharmacology. A comprehensive summary of the existing preparation methods and synthetic materials, such as 5-aminoimidazole-4-carboxamide, guanine and its derivatives, and other purine derivatives, is presented to elucidate the preparation of ACV in detail. In addition, it presents valuable analytical procedures for the toxicological studies of ACV, which are essential for human use and dosing. Analytical methods, including spectrophotometry, high performance liquid chromatography (HPLC), liquid chromatography/tandem mass spectrometry (LC-MS/MS), electrochemical sensors, molecularly imprinted polymers (MIPs), and flow injection–chemiluminescence (FI-CL) are also highlighted. A brief description of the characteristics of each of these methods is also presented. Finally, insight is provided for the development of ACV to drive further innovation of ACV in pharmaceutical applications. This review provides a comprehensive summary of the past life and future challenges of ACV.

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Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Section: High Performance Liquid Chromatography (Hplc)mentioning

confidence: 99%

Critical Review of Synthesis, Toxicology and Detection of Acyclovir

Wei

Yao

et al. 2021

Molecules

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Development and Fabrication of a Molecularly Imprinted Polymer-Based Electroanalytical Sensor for the Determination of Acyclovir

Al Faysal,

Cetinkaya,

Kaya

et al. 2024

ACS Omega

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Acyclovir (ACV), a synthetic nucleoside derivative of purine, is one of the most potent antiviral medications recommended in the specific management of varicella-zoster and herpes simplex viruses. The molecularly imprinted polymer (MIP) was utilized to create an effective and specific electrochemical sensor using a straightforward photopolymerization process to determine ACV. The polymeric thin coating was developed using the template molecule ACV, a functional monomer acrylamide, a basic monomer 2-hydroxyethyl methacrylate, a cross-linker ethylene glycol dimethacrylate, and a photoinitiator 2-hydroxy-2-methyl propiophenone on the exterior of the glassy carbon electrode (GCE). Scanning electron microscopy, attenuated total reflectance−Fourier transform infrared spectroscopy, electrochemical impedance spectroscopy, and cyclic voltammetry were employed for the purpose of characterizing the constructed sensor (AM-ACV@MIP/GCE). Differential pulse voltammetry and a 5 mM ferrocyanide/ferricyanide ([Fe(CN) 6 ] 3−/4− ) redox reagent were used to detect the ACV binding to the specific cavities on MIP. The study involves density functional theory (DFT) calculations, which were conducted to investigate template-functional monomer interactions thoroughly, calculate template-functional monomer interaction energies, and determine the optimal template/functional monomer ratio. DFT calculations were performed using Becke's three-parameter hybrid functional with the Lee−Yang−Parr correlation functional (B3LYP) method and 6-31G(d,p) basis set. The sensor exhibits linear performance throughout the concentration region 1 × 10 −11 to 1 × 10 −10 M, and the limit of detection and limit of quantification were 7.15 × 10 −13 M and 2.38 × 10 −12 M, respectively. For the electrochemical study of ACV, the sensor demonstrated high accuracy, precision, robustness, and a short detection time. Furthermore, the developed electrochemical sensor exhibited exceptional recovery in tablet dosage form and commercial human blood samples, with recoveries of 99.40 and 100.44%, respectively. The findings showed that the AM-ACV@MIP/GCE sensor would effectively be used to directly assess pharmaceuticals from actual specimens and would particularly detect ACV compared to structurally similar pharmaceutical compounds.

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Optimization and Validation Methods for Assay of Acyclovir Cream Determination by High Performance Liquid Chromatography

Cited by 2 publications

References 5 publications

Critical Review of Synthesis, Toxicology and Detection of Acyclovir

Critical Review of Synthesis, Toxicology and Detection of Acyclovir

Development and Fabrication of a Molecularly Imprinted Polymer-Based Electroanalytical Sensor for the Determination of Acyclovir

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