Optimizing immobilization on two-dimensional carboxyl surface: pH dependence of antibody orientation and antigen binding capacity

Fiber optic-SPR platform for fast and sensitive infliximab detection in serum of inflammatory bowel disease patients

“…As shown previously, the efficiency of antibody immobilization depends on several parameters, such as buffer pH, antibody concentration, ionic strength and reaction time. Thus, increased immobilization efficiency can be achieved in a buffer with low ionic strength and pH close to but smaller than the isoelectric point (pI) of the antibody (Pei et al 2010;Pillet et al 1994). Because the pI of the capture antibody, MA-IFX20G2, used in this study was unknown, buffers with low ionic strength (10 mM sodium acetate buffer) and with pH ranging from pH 4.5 to pH 5.5 were tested for covalent immobilization of this antibody at three different concentrations (10, 20 and 40 g/ml).…”

A novel method for preparation of MNP@CS-tethered coenzyme for coupled oxidoreductase system

“…In this work, the NH 2 of MNP@CS was activated by EDC/NHS as shown in Scheme 1. The observed phenomenon was likely caused by activation of azelaic acid, EDC and NHS, for which the surface charges changed due to the presence of the positively charged amino groups of succinimide, according to a study on aminemodified magnetic nanoparticles (Pei et al, 2010). Qiao et al (2011) reported conjugation of catecholamine on magnetic nanoparticles coated with sulfonated chitosan, where a negatively charged state of HSO 3 CS DMSA@MNPs became positively charged due to NH 3 + R form of the catecholamine in surface.…”

Comparative study of antibody immobilization mediated by lipid and polymer fibers

“…However, a high percentage of physically immobilized antibodies can be denatured [8][9][10], and sometimes fewer than 3% of the binding sites remain functional [11]. It is widely accepted that the stable and oriented immobilization of proteins such as antibodies can greatly enhance analyte detection and/or capture [12][13][14][15][16]. A variety of coating and/or linker materials may functionalize surfaces, enhancing the stability, orientation and function retention of immobilized proteins.…”