Osajin

Lišková, Margita; Marek, Jaromı́r; Jankovská, Dagmar; Sukupová, Lada; Žemlička, Milan; Vančo, Ján

doi:10.1107/s1600536805015667

Cited by 5 publications

(2 citation statements)

References 9 publications

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“…Schneid. (Moraceae), and its purity was HPLC-proved (Li‰ková et al 2005). For the testing, pomiferin was always suspended in 2 ml of 0.5% Avicel solution (microcrystalline cellulose).…”

Section: Methodsmentioning

confidence: 99%

Effects of Pomiferin Premedication on the Antioxidant Status of Rats with Ischemia-Reperfused Kidney

Janoštíková¹,

Bartošíková²,

Nečas³

et al. 2005

Acta Vet. Brno

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The aim of this study was to analyze the relationship between the antioxidant effect and the dose of pomiferin during a prophylactic administration. The pathological model for an in vivo experiment was the unilateral ischemia-reperfusion of the laboratory rat kidney. The animals were randomly divided into five groups. Pomiferin was administered orally at doses of 5, 10 and 20 mg·kg -1 , in 2 ml of Avicel solution by a gastric sound once a day to three premedicated groups. A placebo -2 ml of 0.5% Avicel solution -was given to the fourth group, and the fifth group was intact. The premedication lasted 15 days and subsequently ischemia of the left kidney was incited under a general anaesthesia for 60 min. The reperfusion lasted 10 min and it was concluded with blood collection from the left ventricle, and the reperfused kidney was recovered. Selected biochemical markers were assessed in blood: superoxide dismutase, glutathione peroxidase, total antioxidant capacity and malondialdehyde. Kidney tissue samples were used for histopathological examination. Biochemical and histopathological results confirmed the anticipated effects of pomiferin. Pomiferin supported the defensive reactions of the system against free radicals and decreased lipid peroxidation in cell membranes. The relation between the effect and the dose of pomiferin was not linear, and some of the assessed differences between the groups were statistically highly significant. The best results of the biochemical examination were achieved after the administration of pomiferin at the dose of 20 mg·kg -1 . The histopathological results confirmed the dose of 5 mg·kg -1 to be the most effective one.

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“…Schneid. (Moraceae), and its purity was HPLC-proved (Li‰ková et al 2005). For the testing, pomiferin was always suspended in 2 ml of 0.5% Avicel solution (microcrystalline cellulose).…”

Section: Methodsmentioning

confidence: 99%

Effects of Pomiferin Premedication on the Antioxidant Status of Rats with Ischemia-Reperfused Kidney

Janoštíková¹,

Bartošíková²,

Nečas³

et al. 2005

Acta Vet. Brno

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“…Osajin (Fig. A) is the major bioactive isoflavone present in the fruit of Maclura pomifera , commonly referred to as the Osage orange [Wolfrom et al, ; Peterson et al, ; Lišková et al, ]. Fruits of the Osage orange have been traditionally used as a home remedy for pest control and as an insect repellent [Wolfrom et al, ].…”

mentioning

confidence: 99%

Osajin Inhibits Solar UV‐Induced Cyclooxygenase‐2 Expression Through Direct Inhibition of RSK2

Kim

Heo

Lee

2017

J of Cellular Biochemistry

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Solar ultraviolet light (sUV) has been shown to promote the development of skin disorders including inflammation, photoaging, and skin carcinogenesis. Osajin is the major bioactive isoflavone present in the fruit of Maclura pomifera, commonly referred to as the Osage orange. In this study, we observed that osajin inhibited sUV-induced cyclooxygenase (COX)-2 protein expression in both HaCaT and JB6 cells. COX-2 is a major mediator of skin inflammation. sUV activated the transcription factors nuclear factor-κB and activator protein-1 which, in turn, induces COX-2 expression. Osajin inhibited transactivation of these transcription factors. We identified RSK2 as an inhibitory target of osajin by screening against 68 kinases related to inflammation. Osajin binds with RSK2 directly in an ATP-competitive manner. Computer modeling simulated a plausible binding orientation between osajin and RSK2. Osajin inhibited sUV-induced phosphorylation of histone H3, a substrate of RSK2. However, sUV-induced phosphorylation of extracellular signal-regulated kinases, p38 kinase, c-Jun N-terminal kinase and Akt, which are signaling factors upstream of RSK2, was unchanged in the presence of osajin. The anti-inflammatory effects and molecular mechanism of osajin suggest that it may have utility as a functional food for skin health and cosmetic ingredient. J. Cell. Biochem. 118: 4080-4087, 2017. © 2017 Wiley Periodicals, Inc.

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