Osmotic fragility of drug carrier erythrocytes

Sprandel, U.; Zöllner, N.

doi:10.1007/bf01851531

Cited by 22 publications

(8 citation statements)

References 13 publications

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“…Thus drug-loaded erythrocytes were found to be more fragile as compared with normal cells. Attainment of spherical shape by the cells supports the observation of high fragility (Sprandel and Zollner 1985). Such highly fragile cells may be destroyed rapidly in circulation.…”

Section: Resultsmentioning

confidence: 73%

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Erythrocyte based delivery system of primaquine:in vitrocharacterization

Talwar

Jain

1992

Journal of Microencapsulation

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Primaquine phosphate, an antimalarial drug, was loaded in erythrocytes by the process of endocytosis. The encapsulation of 0.1-0.15 mg of drug ml-1 of packed erythrocytes was achieved. The loaded cells attained spherical shape and exhibited higher osmotic fragility and lower resistance to turbulence shock as compared with normal cells. Glutaraldehyde treatment stabilized the cells which were noted to be resistant to the osmotic and turbulence shocks. In vitro release of drug and haemoglobin was also retarded upon treatment of loaded erythrocytes with glutaraldehyde. The studies suggest the potentiality of primaquine-loaded, glutaraldehyde-treated erythrocytes as an intravenous drug delivery system for casual prophylaxis and radical cure of malaria.

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Section: Resultsmentioning

confidence: 73%

“…The study was carried out for normal and drug-loaded erythrocytes as described by Sprandel and Zollner (1985). Cells were incubated for 10 min in sodium chloride solutions of different strengths (0*9,0*85,0.8. .…”

Section: Loading Of Primaquine Phosphatementioning

confidence: 99%

Erythrocyte based delivery system of primaquine:in vitrocharacterization

Talwar

Jain

1992

Journal of Microencapsulation

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“…Ghosts that resealed before the addition of salt were called type-I ghosts, those that resealed after addition of salt, type-II ghosts, and ghosts that could not be resealed properly and remained leaky, type-III ghosts [2]. Several of our studies have established the heterogeneity of hemoglobin-containing reversible resealed carrier erythrocytes concerning life-span in vivo, cell size, cell content, osmotic fragility, deformability, and morphology [15][16][17][18][19][20][21][22]. Therefore, previous research was conducted in order to improve relevant factors influencing the preparation techniques of carrier erythrocytes.…”

Section: Introductionmentioning

confidence: 99%

Temperature-induced shape transformation of carrier erythrocytes

Sprandel

1990

Res. Exp. Med.

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Erythrocytes have been proposed as cellular carriers for enzymes and drugs for protected delivery, slow release, or targeting to specific organs. Several studies have established the heterogeneity of resealed carrier erythrocytes concerning various characteristics, including morphology. Carrier erythrocytes were prepared by hypo-osmotic dialysis and consecutive iso-osmotic resealing at different temperatures, and the morphology of these cells was studied by scanning electron microscopy. Different cell shapes could be prepared by temperature variations during resealing or incubation. All intermediate shapes of echinocytosis were observed at low temperatures or with depleted energy supply but could be reversed to biconcave discocytes. Resealing at 4 degrees C resulted in highest percentage of echinocytes III, spherocytes, and leaky cells. Spontaneous resealing of 20% of the cells was obtained at high lysis temperatures. Combining low temperatures for lysis and high temperatures for resealing and sufficient energy supply are advantageous for highest recovery of biconcave discocytes. Shape of the erythrocyte, is the result of divergent forces, and temperature during resealing was found to be an important factor.

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“…Cytotoxic and antineoplastic drugs have also been entrapped in erythrocytes for targeting purposes (9) or to obtain slow release of therapeutic agents (10). Recently, daunomycin (daunorubicin), a close analog of Adriamycin, has been entrapped within human erythrocytes but found to produce marked perturbation of the loaded erythrocytes (11).…”

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confidence: 99%

“…Methemoglobin was determined according to the procedure of Evelyn and Malloy (19) and thiobarbituric acid-reactive material according to the procedure of Stock and Dormandy (20 (21). Osmotic fragility curves were determined according to Sprandel and Zollner (11).…”

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confidence: 99%

Encapsulation of adriamycin in human erythrocytes.

Flora¹,

Benatti²,

Guida³

et al. 1986

Proc. Natl. Acad. Sci. U.S.A.

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Adriamycin (doxorubicin) was encapsulated in human erythrocytes by means of a dialysis technique involving transient hypotonic hemolysis followed by isotonic resealing. Up to 1.6 mg of the drug was entrapped per ml of packed erythrocytes, with the efficiency of encapsulation being 60-80%. In vitro incubation of the Adriamycin-loaded erythrocytes in autologous plasma was accompanied by progressive release of unaltered Adriamycin in the medium. The efflux was still evident after 50 hr. The metabolism of encapsulated Adriamycin was restricted to limited formation of the C-13 hydroxylated metabolite, adriamycinol, in the normal erythrocytes but not in erythrocytes from individuals deficient in glucose-6-phosphate dehydrogenase (D-glucose-6-phosphate: NADP+ 1-oxidoreductase, EC 1.1.1.49) activity. Reductive bioactivation of encapsulated Adriamycin to yield the corresponding aglycones was not observed in a variety of conditions. However, when NADPH ferredoxin reductase and ferredoxin, both purified from spinach leaves, were co-entrapped within erythrocytes and allowed to catalyze electron transfer to Adriamycin intracellularly under N2, a quantitative conversion to 7-deoxyadriamycin aglycone was obtained. Adriamycinloaded erythrocytes did not show any significant oxidative damage, except for a variable increase of methemoglobin, suggesting some redox cycling between native Adriamycin and its semiquinone radical. Encapsulation of Adriamycin in autologous human erythrocytes may represent a therapeutic strategy for the slow release in circulation of this antineoplastic drug in order to reduce or prevent its adverse effects and especially the delayed cardiotoxicity that limits its use in patients with neoplastic disease.

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Osmotic fragility of drug carrier erythrocytes

Cited by 22 publications

References 13 publications

Erythrocyte based delivery system of primaquine:in vitrocharacterization

Erythrocyte based delivery system of primaquine:in vitrocharacterization

Temperature-induced shape transformation of carrier erythrocytes

Encapsulation of adriamycin in human erythrocytes.

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