Overexpression of SlMYB75 enhances resistance to Botrytis cinerea and prolongs fruit storage life in tomato

Liu, Mengyu; Zhang, Zhen; Xu, Zhixuan; Wang, Lina; Chen, Chunhua; Ren, Zhonghai

doi:10.1007/s00299-020-02609-w

Cited by 30 publications

(15 citation statements)

References 67 publications

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“…Silencing SlMYB70 promotes ethylene production to accelerate fruit ripening and softening [ 89 ]. In addition, the overexpression of SlMYB75 prolongs tomato fruit shelf life by directly down-regulating the expression of SlFSR , which regulates the transcription of genes related to cell wall modification [ 134 ]. The transient overexpression of FvMYB79 in strawberry significantly increases the transcriptional level of FvPME38 , resulting in fruit softening and ripening, while silencing FvMYB79 delays fruit ripening and increases fruit firmness [ 135 ].…”

Section: Transcriptional Regulation Of Fruit Softeningmentioning

confidence: 99%

Molecular and Genetic Events Determining the Softening of Fleshy Fruits: A Comprehensive Review

Peng

Liu

et al. 2022

IJMS

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Fruit softening that occurs during fruit ripening and postharvest storage determines the fruit quality, shelf life and commercial value and makes fruits more attractive for seed dispersal. In addition, over-softening results in fruit eventual decay, render fruit susceptible to invasion by opportunistic pathogens. Many studies have been conducted to reveal how fruit softens and how to control softening. However, softening is a complex and delicate life process, including physiological, biochemical and metabolic changes, which are closely related to each other and are affected by environmental conditions such as temperature, humidity and light. In this review, the current knowledge regarding fruit softening mechanisms is summarized from cell wall metabolism (cell wall structure changes and cell-wall-degrading enzymes), plant hormones (ETH, ABA, IAA and BR et al.), transcription factors (MADS-Box, AP2/ERF, NAC, MYB and BZR) and epigenetics (DNA methylation, histone demethylation and histone acetylation) and a diagram of the regulatory relationship between these factors is provided. It will provide reference for the cultivation of anti-softening fruits.

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Section: Transcriptional Regulation Of Fruit Softeningmentioning

confidence: 99%

Molecular and Genetic Events Determining the Softening of Fleshy Fruits: A Comprehensive Review

Peng

Liu

et al. 2022

IJMS

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“…For example, A. thaliana phyB and SDG8 genes participate in the resistance defense against B. cinerea by regulating JA biosynthesis [39,40]. In addition, SlMYB75 plays an active role in the resistance of tomato to B. cinerea by regulating the JA signaling pathway [38]. Therefore, we speculated that the JA signaling pathway may be essential in VaCRK2-mediated resistance against B. cinerea.…”

Section: Discussionmentioning

confidence: 96%

“…In this study, several genes related to JA signaling pathway were upregulated in A. thaliana VaCRK2 overexpression lines compared with A. thaliana wildtype Col-0 after B. cinerea inoculation (Figure 4, Figures S2 and S8). The JA signaling pathway has been associated with gray mold resistance in different plants, including A. thaliana, cucumber and tomato [36][37][38]. For example, A. thaliana phyB and SDG8 genes participate in the resistance defense against B. cinerea by regulating JA biosynthesis [39,40].…”

Section: Discussionmentioning

confidence: 99%

VaCRK2 Mediates Gray Mold Resistance in Vitis amurensis by Activating the Jasmonate Signaling Pathway

Gao

Tang

et al. 2021

Agronomy

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Cysteine-rich receptor-like kinases (CRKs) are ubiquitous plant receptor-like kinases, which play a significant role in plant disease resistance. Gray mold is an economically important disease of grapes caused by Botrytis cinerea. However, CRK genes and their function in gray mold disease resistance in grapes have not been elucidated. This study aimed to identify and characterize CRKs in grapes and determine their role in gray mold resistance. Four CRKs were identified in Vitis amurensis and named VaCRK1–VaCRK4 according to their genomic distribution. The four VaCRKs were ectopically expressed in Arabidopsis thaliana to study their function in defense response against B. cinerea. Heterologous expression of VaCRK2 in A. thaliana conferred resistance to B. cinerea. VaCRK2 expression in gray mold-resistant grape cultivar increased significantly after B. cinerea inoculation and methyl jasmonate treatment. Furthermore, the expression of jasmonic acid (JA) signaling pathway-related genes in VaCRK2 overexpression lines of A. thaliana was significantly increased after B. cinerea inoculation, leading to the upregulation of pathogenesis-related (PR) genes and reactive oxygen species (ROS) accumulation. Overall, these results suggest that VaCRK2 confers resistance to B. cinerea by activating PR gene expression and oxidative burst through the JA signaling pathway.

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“…TFs also influence infection of fruit by fungal pathogens [ 1 , 10 ]. These include a GRAS TF SlFSR [ 11 ], SlMYB75 [ 12 ], bHLH TF MYC2 [ 13 , 14 ] and an AP2 TF SlSHN3 [ 15 ]. Other TFs involved in fruit development, but not directly related to ripening, can also impact fruit susceptibility to Botrytis cinerea ( B. cinerea ), such as the KNOX TFs (SlTKN4 and SlTKN2), Golden2-like (SlGLK2) and TFs and SlAPRR2-like, which regulates chlorophyll accumulation and other aspects of chloroplast development before ripening onset [ 16 , 17 , 18 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

Contrasting Roles of Ethylene Response Factors in Pathogen Response and Ripening in Fleshy Fruit

et al. 2022

Cells

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Fleshy fruits are generally hard and unpalatable when unripe; however, as they mature, their quality is transformed by the complex and dynamic genetic and biochemical process of ripening, which affects all cell compartments. Ripening fruits are enriched with nutrients such as acids, sugars, vitamins, attractive volatiles and pigments and develop a pleasant taste and texture and become attractive to eat. Ripening also increases sensitivity to pathogens, and this presents a crucial problem for fruit postharvest transport and storage: how to enhance pathogen resistance while maintaining ripening quality. Fruit development and ripening involve many changes in gene expression regulated by transcription factors (TFs), some of which respond to hormones such as auxin, abscisic acid (ABA) and ethylene. Ethylene response factor (ERF) TFs regulate both fruit ripening and resistance to pathogen stresses. Different ERFs regulate fruit ripening and/or pathogen responses in both fleshy climacteric and non-climacteric fruits and function cooperatively or independently of other TFs. In this review, we summarize the current status of studies on ERFs that regulate fruit ripening and responses to infection by several fungal pathogens, including a systematic ERF transcriptome analysis of fungal grey mould infection of tomato caused by Botrytis cinerea. This deepening understanding of the function of ERFs in fruit ripening and pathogen responses may identify novel approaches for engineering transcriptional regulation to improve fruit quality and pathogen resistance.

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Overexpression of SlMYB75 enhances resistance to Botrytis cinerea and prolongs fruit storage life in tomato

Cited by 30 publications

References 67 publications

Molecular and Genetic Events Determining the Softening of Fleshy Fruits: A Comprehensive Review

Molecular and Genetic Events Determining the Softening of Fleshy Fruits: A Comprehensive Review

VaCRK2 Mediates Gray Mold Resistance in Vitis amurensis by Activating the Jasmonate Signaling Pathway

Contrasting Roles of Ethylene Response Factors in Pathogen Response and Ripening in Fleshy Fruit

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