Oxidative and conjugative metabolism of diethylstilbestrol by rabbit preimplantation embryos

Balling, Rudi; Haaf, Heidemarie; Maydl, Regina; Metzler, Manfred; Beier, Henning M.

doi:10.1016/0012-1606(85)90462-2

Cited by 24 publications

(6 citation statements)

References 10 publications

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“…Manes [37] suggested that a substantial portion of the OXPHOS-independent oxygen consumption by blastocysts contributes to surface H 2 O 2 production and, indeed, Manes and Lai [17] determined that 30% of the total oxygen consumed by blastocysts was utilized by H 2 O 2 -producing oxygenases. Because cleavage-stage embryos produce only limited H 2 O 2 [38] (unpublished observations), the two mixed multifunctional oxidase systems maturing in cleavage-stage embryos [38,39] might account for the OXPHOS-independent oxygen consumption at this developmental stage.…”

Section: Discussionmentioning

confidence: 96%

Oxidative Phosphorylation-Dependent and -Independent Oxygen Consumption by Individual Preimplantation Mouse Embryos1

Trimarchi

Liu

Porterfield

et al. 2000

Biology of Reproduction

233

138

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The self-referencing electrode technique was employed to noninvasively measure gradients of dissolved oxygen in the medium immediately surrounding developing mouse embryos and, thereby, characterized changes in oxygen consumption and utilization during development. A gradient of depleted oxygen surrounded each embryo and could be detected >50 microm from the embryo. Blastocysts depleted the surrounding medium of 0.6+/-0.1 microM of oxygen, whereas early cleavage stage embryos depleted the medium of only 0.3+/-0.1 microM of oxygen, suggesting a twofold increase in oxygen consumption at the blastocyst stage. Mitochondrial oxidative phosphorylation (OXPHOS) accounted for 60-70% of the oxygen consumed by blastocysts, while it accounted for only 30% of the total oxygen consumed by cleavage-stage embryos. The amount of oxygen consumed by non-OXPHOS mechanisms remained relatively constant throughout preimplantation development. By contrast, the amount of oxygen consumed by OXPHOS in blastocysts is greater than that consumed by OXPHOS in cleavage-stage embryos. The amount of oxygen consumed by one-cell embryos was modulated by the absence of pyruvate from the culture medium. Treatment of one-cell embryos and blastocysts with diamide, an agent known to induce cell death in embryos, resulted in a decline in oxygen consumption, such that the medium surrounding dying embryos was not as depleted of oxygen as that surrounding untreated control embryos. Together these results validate the self-referencing electrode technique for analyzing oxygen consumption and utilization by preimplantation embryos and demonstrate that changes in oxygen consumption accompany important physiological events, such as development, response to medium metabolites, or cell death.

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Section: Discussionmentioning

confidence: 96%

Oxidative Phosphorylation-Dependent and -Independent Oxygen Consumption by Individual Preimplantation Mouse Embryos1

Trimarchi

Liu

Porterfield

et al. 2000

Biology of Reproduction

233

138

View full text Add to dashboard Cite

show abstract

“…Macrophages cultured with mouse blastocysts have been shown in ultrastructural studies to enter the cytoplasm of trophectoderm cells and to pass between ICM cells; there was no evidence that they exerted any cytotoxic effect (Tachi et al, 1985). Belling et al (1985) have found that 5-to 6-day rabbit blastocysts can conjugate and actively metabolize diethylstilboestrol, and infer that there are at this stage specific enThe cleaving eutherian egg and the blastocyst 109 zyme activities, of which one, monooxygenase, is not expressed in the adult animal. Other aspects of metabolism in cleaving eggs and blastocysts are discussed under 'Recovery, culture, storage and transfer' in section 2.4.1.…”

Section: Metabolismmentioning

confidence: 97%

Pre-Implantation Development

Austin¹

1994

Marshall’s Physiology of Reproduction

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“…Maternal use of diethylstilbestrol prior to gestational age of 18 weeks was associated later with incidences ( < 0.1%) of vaginal clear cell adenocarcinoma of female offspring at puberty and non -adenocarcinoma reproductive tract problems in an estimated 95% of female offspring (Herbst, Ulfelder and Poskanzer, 1971 ;Melnick et al 1987 ). A genotoxic epoxide intermediate of diethylstilbestrol formed by fetal CYPs during gestation is thought to be responsible for the subsequent carcinogenesis at puberty (Balling et al , 1985 ).…”

Section: Carcinogenesismentioning

confidence: 99%