p63 is useful in the diagnosis of mammary metaplastic carcinomas

“…Figure 3b upper panel depicts typical immunostaining of a squamous lesion for the basal cytokeratin, CK6 which is a marker of stem and basaloid progenitor cells and the basal cell marker, p63 which is present in both squamous and sarcomatoid metaplasias (Tse et al, 2006). Cells constituting the basaloid islands within squamous metaplasias contained both CK6 (shown) and p63-positive cells (not shown) as did areas of squamous differentiation with extensive keratinization shown immunostained for p63.…”

Section: Mmtv-ikbamentioning

confidence: 98%

The canonical NF-κB pathway is required for formation of luminal mammary neoplasias and is activated in the mammary progenitor population

et al. 2009

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The role of the canonical NF-kB pathway in mammary tumorigenesis was investigated using a transgenic (TG) mouse expressing a dominant-negative inhibitor of kB (IkBa SR (S32A/S36A) ) in the mammary gland under the control of the mouse mammary tumor virus promoter (MMTV). TG and control mice were subjected to a chemical carcinogenesis protocol. Hyperkeratinized squamous metaplasias (cytokeratinÀ6 þ /p63 þ ) sometimes with a basaloid island component, were found in both TG and control mice whereas luminal (cytokeratin-19 þ / MUC1 þ ) ErbB2 þ papillary and adenomatous lesions developed almost exclusively in control mice. p65/RelAand NF-kB DNA-binding activity were detected in mammary luminal lesions, but rarely in squamous metaplasias. Analysis of NF-kB family proteins and target genes using microarray data from a cohort of human mammary tumors revealed the expression of a canonical NF-kB pathway, but not non-canonical pathway proteins in HER2 þ luminal cancers. HER2 þ tumors also showed differential regulation of specific NF-kB target genes relative to basal and ER þ luminal cancers. Isolation of mammary cell populations enriched for stem and progenitor cell characteristics from an NF-kB-EGFP reporter mouse by fluorescence-activated cell sorting demonstrated that luminal progenitors contain activated NF-kB whereas the mammary stem cell-enriched population, does not. Together these data suggest that the canonical NF-kB pathway is active in normal luminal progenitor cells before transformation and is required for the formation of mammary luminal-type epithelial neoplasias.

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“…Therefore, it can be said that the distribution of cytoplasmic p63 immunoexpression may be affected by age, especially in breast carcinoma with basal-like type. Unlike the results of this research, the p63 was used as the myoepithelial marker to diagnose metaplastic carcinoma with a sensitivity of 65%, a specificity of 96%, a positive predictive value of 96%, a negative predictive value of 66 %, and an accuracy of 78% [18].…”

Section: Statistical Analysesmentioning

confidence: 79%

“…Pregnancy is often associated with breast cancer, generating mortality risk [18]. Conducting prognosis is one of the efforts to assess the development of breast cancer [19].…”

Section: Discussionmentioning

confidence: 99%

Frequency and Distribution of Cytoplasmic and Nuclear P63 in Recognizing the Basal-Like and Non-Basal-Like Types of Breast Cancer Based on Age.

Kamarlis

Lubis

Hernowo

et al. 2018

Asian J Pharm Clin Res

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Objective: To assess the immune expressions of cytoplasmic and nuclear protein63 (p63) proteins in metaplastic triple-negative breast cancer (TNBC) patients with basal-like and non-basal-like types based on age.Methods: Forty samples of breast cancer patients diagnosed with TNBC were examined immune histochemical based on the immune expressions of cytoplasmic and nuclear p63 with basal-like (CK 5/6, positive) and non-basal-like (CK 5/6 negative) types. The histoscore values were categorized into Score 1 (<20%, negative), Score 2 (>20% -50%, weak positive), Score 3 (>50% -80%, moderate positive), and Score 4 (>80 %, strong positive) also analyzed by one-way analysis variance, probability (p<0.05), and correlation (p<0.01).Results: Forty subjects with breast cancer showed 23 of basal-like (57.5%) and 17 of non-basal-like (42.5%) types. The cytoplasmic p63 had a low histoscores in the group of < 40-49 years old (55%), the moderate group of 40-55 years old (27.5%), and the high group of fewer than 50-60 years old (17.5%). The nuclear p63 was low in all age groups (p<0.05). It was the significant difference between basal-like and non-basal-like types (p<0.01) and strongest correlation (1.00). The immunoexpression of cytoplasmic p63 of basal-like cell type had an intensity of 88.2%, while nuclear p63 possessed 11.8% (p<0.05). Conclusion:The frequency of cytoplasm p63 in all age groups of metaplastic breast cancer of basal-like type was more dominant than the nuclear p63. Otherwise, in the non-basal-like type, the cytoplasmic p63 protein was lower than the of nuclear p63 protein.

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p63 is useful in the diagnosis of mammary metaplastic carcinomas

Cited by 73 publications

References 24 publications

The cell cycle regulator ecdysoneless cooperates with H-Ras to promote oncogenic transformation of human mammary epithelial cells

The cell cycle regulator ecdysoneless cooperates with H-Ras to promote oncogenic transformation of human mammary epithelial cells

The canonical NF-κB pathway is required for formation of luminal mammary neoplasias and is activated in the mammary progenitor population

Frequency and Distribution of Cytoplasmic and Nuclear P63 in Recognizing the Basal-Like and Non-Basal-Like Types of Breast Cancer Based on Age.

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