Paeoniflorin alleviates non-alcoholic steatohepatitis in rats: Involvement with the ROCK/NF-κB pathway

Ma, Zhihong; Chu, Li; Liu, Hongying; Li, Jieru; Zhang, Yuanyuan; Liu, Wentai; Dai, Jun; Yi, Jianfeng; Gao, Yue

doi:10.1016/j.intimp.2016.06.023

Cited by 49 publications

(33 citation statements)

References 40 publications

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“…Our study also demonstrated that treatment with PF significantly reduced lipid accumulation in liver, which is consistent with our previous study11 and the report by Zhang et al 14. in animals.…”

Section: Discussionsupporting

confidence: 93%

“…The Control group rats were fed a standard diet, and the HFD and PF groups received the HFD, which was standard chow plus 2% cholesterol and 15% lard)32. Ten weeks later, the rats in the PF and HFD groups were gavaged with PF at a dose of 20 mg/kg/day11 or with an equal volume of distilled water, respectively.…”

Section: Methodsmentioning

confidence: 99%

“…1), a major pharmacologically active component from roots of peonies, has been reported to possess anti-inflammatory7, antioxidant8, antihyperlipidemic activities9 and hepatoprotection10, with low toxicity and few side effects1112. Recent studies indicated that PF improved insulin resistance (IR) in NAFLD animal models by promoting fatty acid oxidation through the activation of peroxisome proliferator-activated receptor-alpha (PPARα)13 and by increasing the activity of AMP-activated protein kinase (AMPK)14.…”

mentioning

confidence: 99%

“…Recent studies indicated that PF improved insulin resistance (IR) in NAFLD animal models by promoting fatty acid oxidation through the activation of peroxisome proliferator-activated receptor-alpha (PPARα)13 and by increasing the activity of AMP-activated protein kinase (AMPK)14. Our previous research demonstrated that PF had anti-inflammatory and hepatoprotective effects in NASH rats and that these effects due to inhibition of the Rho kinase/NF-κB signaling pathway in liver with NASH11.…”

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confidence: 99%

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Beneficial effects of paeoniflorin on non-alcoholic fatty liver disease induced by high-fat diet in rats

Chu

Liu

et al. 2017

Sci Rep

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Non-alcoholic fatty liver disease (NAFLD) is the most prevalent form of chronic liver diseases. This study sought to evaluate the insulin-sensitizing effect of paeoniflorin (PF) on high-fat diet-induced NAFLD and possible molecular mechanisms. Male Sprague Dawley rats were fed a high-fat diet (HFD) for 10 weeks to establish the NAFLD model, and PF (20 mg/kg/d) was gavaged to the NAFLD rats for another four weeks. Our results demonstrated that HFD resulted in hepatocellular ballooning, micro-/macrovesicular steatosis, and oxidative stress in the liver, accompanied by increased serum total cholesterol (TC), triglyceride (TG), free fatty acid (FFA), alanine aminotransferase (ALT), and aspartate aminotransferase (AST) levels and homeostasis model of insulin resistance (HOMA-IR) index. PF treatment improved the biochemical and histopathological changes in NAFLD rats. Moreover, we also found that PF could inhibit lipid ectopic deposition via regulating lipid metabolism (inhibiting lipid synthesis of cholesterol and de novo pathway), and exert insulin sensitizing effect by regulating the insulin signaling pathway IRS/Akt/GSK3β and anti-oxidation. The study findings suggest that PF has therapeutic potential against NAFLD and that it acts through multiple signaling pathways.

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Section: Discussionsupporting

confidence: 93%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Beneficial effects of paeoniflorin on non-alcoholic fatty liver disease induced by high-fat diet in rats

Chu

Liu

et al. 2017

Sci Rep

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“…Therefore, cognitive dysfunction in patients with diabetes is common, and diabetes can induce dementia in the most severe cases. However, due to the population aging and social support, and the lack of primary health care systems, dementia has not received sufficient attention (18). In the present study, it was found that salidroside increased body weight, inhibited serum blood glucose, and prevented cognitive impairment in rats with diabetes.…”

Section: Discussionmentioning

confidence: 54%

Salidroside prevents diabetes‑induced cognitive impairment via regulating the Rho pathway

2018

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In previous years, it has been found that Rhodiola has a wide range of pharmacological effects in diseases of the cardiovascular system, as it can remove superoxide anions and hydroxyl radicals in chemical reactions. Behavioral assessment was used to measure cognitive impairment. Inflammation, oxidative stress and caspase-3 activity were measured using commercial kits. Western blot analysis was used to measure Rho/Rho-associated kinase (ROCK)/sirtuin 1 (SIRT1)/nuclear factor (NF)-κB protein expression. The objective of the present study was to investigate the protective effect of salidroside on diabetes and diabetes-induced cognitive impairment. The results of the study demonstrated that salidroside prevented cognitive impairment, decreased serum blood glucose levels and increased body weight, reduced fasting blood glucose levels and blood lipid levels, and inhibited oxidative stress, inflammation and nerve cell apoptosis in the diabetic rat model. Salidroside suppressed ROCK/ SIRT1 NF-κB pathway and protein expression in the diabetic rats. These data showed that salidroside prevented diabetes-induced cognitive impairment by regulating the Rho/ROCK/SIRT1/NF-κB pathway.

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Simultaneous determination of five compounds of fried Radix Paeoniae Alba extract in beagle dogs plasma by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry and its application in a pharmacokinetic study

Cheng,

Yuan,

et al. 2023

Biomedical Chromatography

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In this present study, we developed a reliable and simple ultra‐performance liquid chromatography tandem mass spectrometry (UPLC–MS/MS) assay for the simultaneous quantification of paeoniflorin, albiflorin, oxypaeoniflorin, benzoylpaeoniflorin and isomaltopaeoniflorin in beagle dog plasma. We also analyzed the pharmacokinetics of those components after oral administration of fried Radix Paeoniae Alba (FRPA) in beagle dogs. Plasma samples were processed by protein precipitation with methanol. Chromatographic separation was performed with a Waters HSS‐T3 C18 column (100 × 2.1 mm, 1.8 μm, kept at 40°C) using multiple reaction monitoring mode. A gradient elution procedure was used with solvent A (0.02% formic acid–water) and solvent B (0.02% formic acid–acetonitrile) as mobile phases. Method validation was performed as US Food and Drug Administration guidelines, and the results met the acceptance criteria. The method we establish in this experiment was successfully applied to the pharmacokinetic study after oral administration of FRPA extract to beagle dogs.

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Paeoniflorin alleviates non-alcoholic steatohepatitis in rats: Involvement with the ROCK/NF-κB pathway

Cited by 49 publications

References 40 publications

Beneficial effects of paeoniflorin on non-alcoholic fatty liver disease induced by high-fat diet in rats

Beneficial effects of paeoniflorin on non-alcoholic fatty liver disease induced by high-fat diet in rats

Salidroside prevents diabetes‑induced cognitive impairment via regulating the Rho pathway

Simultaneous determination of five compounds of fried Radix Paeoniae Alba extract in beagle dogs plasma by Ultra Performance Liquid Chromatography Tandem Mass Spectrometry and its application in a pharmacokinetic study

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