Pain during topical photodynamic therapy in Pakistani and Afghani patients

Khan, Rahmat Ullah; Khurshid, Nauman; Ikram, Manzoor; Firdous, S.

doi:10.1016/j.pdpdt.2011.03.076

Cited by 3 publications

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“…ALA is the pro drug of the photosensi tizer protoporphyrin IX (PPIX) [16,17]. After ALA administration, cells generate PPIX through the haem biosynthetic pathway.…”

Section: Introductionmentioning

confidence: 99%

In vitro study of cell death with 5-aminolevulinic acid based photodynamic therapy to improve the efficiency of cancer treatment

et al. 2012

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Photodynamic therapy (PDT) is a kind of photochemo therapeutic treatment that exerts its effect mainly through the induction of cell death. Distinct types of cell death may be elicited by different PDT regimes. In this study, efforts are underway to optimize PDT protocols for improved efficacy and combination of all three PDT mechanisms involved in the different human carcinomas cell narcosis. Our in vitro cell cul ture experiments with 5 aminolevulanic acid (ALA) a clinically approved photiosensitizer (PS) and 635 nm laser light have yielded promising results, as follow: (1) (human cervical cancer (HeLa) cell line incubated, for 18 h, with 30 µg/ml of 5 ALA, treated with laser light dose of 50 J/cm 2 can produce 85% of cell killing (2) human larynx carcinoma (Hep2c) cell line incubated, for 7 h, with 55 µg/ml of 5 ALA, treated with laser light dose of 85 J/cm 2 can produce 75% of cell killing (3) human liver cancer (HepG2) cell line incubated, for 22-48 h, with 262 µg/ml of 5 ALA, treated with laser light dose of 120 J/cm 2 can produce 95% of cell killing (4) human muscle cancer (RD) cell line incubated, for 47 h, with 250 µg/ml of 5 ALA, treated with laser light dose of 80 J/cm 2 can produce 76% of cell killing (5) Human embryonic kidney (HEK293T) cell line incu bated, for 18 h, with 400 µg/ml of 5 ALA, treated with laser light dose of 40 J/cm 2 can produce 82% of cell killing confirming the efficacy of photodynamic therapy.

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“…ALA is the pro drug of the photosensi tizer protoporphyrin IX (PPIX) [16,17]. After ALA administration, cells generate PPIX through the haem biosynthetic pathway.…”

Section: Introductionmentioning

confidence: 99%

In vitro study of cell death with 5-aminolevulinic acid based photodynamic therapy to improve the efficiency of cancer treatment

et al. 2012

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“…The presented techniques can be used for new light-based diagnostic apparatus for the quantities analysis of dengue patients. In our previous research findings, we have characterized tissues and biomaterials for optical imaging to diagnose diseases and develop optical detection equipments [35][36][37][38][39][40][41][42][43][44][45][46][47][48][49][50][51][52][53].…”

Section: Confocal Microscopic Analysismentioning

confidence: 99%

Optical Diagnostic of Dengue Virus Infected Human Blood using Raman, Polarimetric and Fluorescence Spectroscopy

Firdous¹,

Anwar²

2017

Dengue - Immunopathology and Control Strategies

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In this chapter, we present the optical diagnosis of normal and dengue viral-infected human blood using Raman, Polarimetric, Transmission, and Fluorescence Spectroscopic techniques. The possibility of using light in diagnosis and treating illness has been known for thousands of years. The properties of light and lasers provided many modern applications at home, in industry, and in the field of medicine. Laser use in the field of medicine is large and steadily growing. This growth is based on the versatility of laser light. Efficient and accurate diagnosis of dengue is of primary importance for clinical care. A range of laboratory diagnostic methods has been developed to support patient management and disease control. The choice of diagnostic method depends on the purpose for which the testing is done, the type of laboratory facilities and technical expertise available, costs, and the time of sample collection. The dengue viral infection is mostly diagnosed through laboratory tests; these tests include detection of the virus, virus antigen, anti-dengue virus antibody, complement fixation test, neutralization tests, and detection of virus nucleic acid. As dengue infection most rapidly increases in different regions, early diagnostic confirmation of dengue infection in patients allows for timely clinical intervention, etiological investigation, and disease control. Hence, diagnosis of dengue disease during the acute phase should be a priority and is a public health concern. Lasers and optics have many applications in medical sciences; diagnosis and treatment of diseases with lasers and light are latest and noninvasive techniques. Development of light-based apparatus has evolved into tools for improved diagnosis and treatment modalities in medical sciences. The methods of the laser spectroscopy make it possible to obtain direct information regarding the structure and dynamics of the functional groups of biomolecules. Development of new light sources, optics, and diode laser of different wavelengths makes them attractive for spectroscopy of biological molecules. In our study, more than 600 dengue viral-infected blood or blood sera samples and 25 non-dengue healthy blood samples were analyzed using four different optical methodologies. In the first study, Raman spectrum peaks for normal samples observed at 1527, 1170, and 1021 cm −1 show the presence of different biological materials, including lipids, carbohydrates, skeletal CC stretch of acyl chains, and

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In vivo study of laser irradiation of fractionated drug administration based mechanism for effective photodynamic therapy in rat liver

et al. 2011

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Pain during topical photodynamic therapy in Pakistani and Afghani patients

Cited by 3 publications

References 0 publications

In vitro study of cell death with 5-aminolevulinic acid based photodynamic therapy to improve the efficiency of cancer treatment

In vitro study of cell death with 5-aminolevulinic acid based photodynamic therapy to improve the efficiency of cancer treatment

Optical Diagnostic of Dengue Virus Infected Human Blood using Raman, Polarimetric and Fluorescence Spectroscopy

In vivo study of laser irradiation of fractionated drug administration based mechanism for effective photodynamic therapy in rat liver

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