Pancreatic Extracts in Treatment of Diabetes Mellitus

Banting,; Best,; Collip,; Campbell, Matthew D.; Fletcher,

doi:10.1097/00005053-192401000-00013

Cited by 128 publications

(151 citation statements)

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“…They realised that they had created an animal model of diabetes, and correctly concluded that the pancreas must secrete an 'anti-diabetogenic factor' (later found to be insulin) that enables the body to utilise glucose [1]. In 1921, a diabetic dog became the first recipient of insulin therapy [2], paving the way for the treatment of human patients. Diabetic Beagle dogs (in whom diabetes is usually induced by pancreatectomy) are still used for research, particularly in islet cell transplantation studies [3], although rodents, such as the non-obese diabetic mouse and BB rat, have long replaced the dog as the major animal models of diabetes.…”

Section: Introductionmentioning

confidence: 99%

Canine diabetes mellitus: can old dogs teach us new tricks?

et al. 2005

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Section: Introductionmentioning

confidence: 99%

Canine diabetes mellitus: can old dogs teach us new tricks?

et al. 2005

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“…The presence of insulin-like proteins in plants has been investigated since the discovery of insulin in the pancreas of dogs early in the last century (1)(2)(3). Collip (2) reported on the hypoglycemic activity of extracts of several plants when administered to normal rabbits and depancreatized dogs.…”

Section: Introductionmentioning

confidence: 99%

Isolation and intracellular localization of insulin-like proteins from leaves of Bauhinia variegata

Azevedo

Maciel

Silva

et al. 2006

Braz J Med Biol Res

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Evidence based on immunological cross-reactivity and anti-diabetic properties has suggested the presence of insulin-like peptides in plants. The objective of the present study was to investigate the presence of insulin-like proteins in the leaves of Bauhinia variegata ("pata-de-vaca", "mororó"), a plant widely utilized in popular medicine as an anti-diabetic agent. We show that an insulin-like protein was present in the leaves of this plant. A chloroplast protein with a molecular mass similar to that of bovine insulin was extracted from 2-mm thick 15% SDS-PAGE gels and fractionated with a 2 x 24 cm Sephadex G-50 column. The activity of this insulin-like protein (0.48 mg/mL) on serum glucose levels of four-week-old Swiss albino (CF1) diabetic mice was similar to that of commercial swine insulin used as control. Further characterization of this molecule by reverse-phase hydrophobic HPLC chromatographic analysis as well as its antidiabetic activity on alloxan-induced mice showed that it has insulin-like properties. Immunolocalization of the insulin-like protein in the leaves of B. variegata was performed by transmission electron microscopy using a polyclonal anti-insulin human antibody. Localization in the leaf blades revealed that the insulin-like protein is present mainly in chloroplasts where it is also found associated with crystals which may be calcium oxalate. The presence of an insulin-like protein in chloroplasts may indicate its involvement in carbohydrate metabolism. This finding has strengthened our previous results and suggests that insulin-signaling pathways have been conserved through evolution. Correspondence

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“…The introduction of insulin therapy in 1922 by Frederick Banting and Charles Best revolutionized the treatment of patients with diabetes and prolonged the lives of millions of people 1,2 . While insulin therapy treats patients with diabetes, it does not cure the disease, nor does it prevent the development of secondary complications associated with long-term diabetes.…”

Section: History Of Pancreatic Islet Transplantation For Type-1 Diabetesmentioning

confidence: 99%

Clinical Islet Cell Transplantation – Recent Advances

Saravanan¹,

Loganathan²,

Naziruddin³

et al. 2017

Current Science

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Type-1 diabetes mellitus (T1DM) caused by autoimmune destruction of insulin-producing beta-cells essentially requires treatment with exogenous insulin therapy. Despite the education, technology and improvements in insulin formulations, patients face the ongoing life-threatening hypoglycaemia with poor quality of life as well as progressive disease leading to microand macro-vascular complications of diabetes. Islet transplantation offers an alternative therapeutic option for these patients. In this review, we discuss the recent advancements in this field tracking from the history of pancreatic islet transplantation to the present-day challenges of clinical islet cell transplantation. We summarize the cutting-edge clinical research with special reference to the results of current trials, including Clinical Islet Transplant Consortium, improvements in immunosuppressive protocols, the need for beta-cell replacement therapies, including the application of induced pluripotent stem cells and mesenchymal stem cells.

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Pancreatic Extracts in Treatment of Diabetes Mellitus

Cited by 128 publications

References 0 publications

Canine diabetes mellitus: can old dogs teach us new tricks?

Canine diabetes mellitus: can old dogs teach us new tricks?

Isolation and intracellular localization of insulin-like proteins from leaves of Bauhinia variegata

Clinical Islet Cell Transplantation – Recent Advances

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