Parthenogenetic activation of mouse oocytes by strontium chloride: A search for the best conditions

Ma, Suo-Feng; Liu, Xinyong; Miao, De-Qiang; Han, Zheng-Bin; Zhang, Xia; Miao, Yi‐Liang; Yanagimachi, Ryuzo; Tan, Jing-He

doi:10.1016/j.theriogenology.2005.03.002

Cited by 81 publications

(63 citation statements)

References 51 publications

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“…This resulted in diploid zygotes with two pronuclei (14,15,17,35,37). The present results show that CB did not affect chromosomal movement or nuclear division but did inhibit spindle rotation and, thus, cytokinesis.…”

Section: Discussionmentioning

confidence: 48%

“…Cytochalasin B, a microfilament-depolymerizing drug widely used in animal cloning (35,36) and parthenogenetic activation (14,15), can inhibit polar body extrusion and therefore chromosome expulsion; in the presence of cytochalasin B, chromosome segregation occurred, but spindle rotation and cytokinesis did not take place. This resulted in diploid zygotes with two pronuclei (14,15,17,35,37).…”

Section: Discussionmentioning

confidence: 99%

“…A variety of artificial stimuli such as ethanol, electroporation, calcium ionophore, ionomycin, and inositol 1,4,5-trisphosphate (InsP 3 ) are used to induce the parthenogenetic activation of mammalian oocytes (11)(12)(13)(14)(15). Yang et al (13) reported that combined treatment with 7% ethanol for 10 min and cycloheximide, a protein synthesis inhibitor, improved the rate of activation of the young oocytes of cattle.…”

Section: Introductionmentioning

confidence: 99%

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Effects of the conditioned medium of mesenchymal stem cells on mouse oocyte activation and development

Feng

Zhou

Ling

et al. 2009

Braz J Med Biol Res

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Mesenchymal stem cells (MSCs) have been reported to secrete a variety of cytokines and growth factors acting as trophic suppliers, but little is known regarding the effects of conditioned medium (CM) of MSCs isolated from femurs and tibias of mouse on the artificial activation of mouse oocytes and on the developmental competence of the parthenotes. In the current study, we investigated the effect of CM on the events of mouse oocyte activation, namely oscillations of cytosolic calcium concentration ([Ca 2+ ] i ), meiosis resumption, pronucleus formation, and parthenogenetic development. The surface markers of MSCs were identified with a fluorescence-activated cell sorter. The dynamic changes of the spindle and formation of pronuclei were examined by laser-scanning confocal microscopy. Exposure of cumulus-oocyte complexes to CM for 40 min was optimal for inducing oocyte parthenogenetic activation and evoking [Ca 2+ ] i oscillations similar to those evoked by sperm (95 vs 100%; P > 0.05). Parthenogenetically activated oocytes immediately treated with 7.5 μg/mL cytochalasin B (CB), which inhibited spindle rotation and second polar body extrusion, were mostly diploid (93 vs 6%, P < 0.01) while CB-untreated oocytes were mostly haploid (5 vs 83%, P < 0.01). Consequently, the blastocyst rate was higher in the CB-treated than in the CB-untreated oocytes. There was no significant difference in developmental rate between oocytes activated with CM and 7% ethanol (62 vs 62%, P > 0.05), but the developmental competence of the fertilized oocytes was superior to that of the parthenotes (88 vs 62%, P < 0.05). The present results demonstrate that CM can effectively activate mouse oocytes, as judged by the generation of [Ca 2+ ] i oscillations, completion of meiosis and parthenogenetic development.

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Section: Discussionmentioning

confidence: 48%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Effects of the conditioned medium of mesenchymal stem cells on mouse oocyte activation and development

Feng

Zhou

Ling

et al. 2009

Braz J Med Biol Res

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“…Oocytes need to be primed with estradiol to develop Ca 2+ oscillations during maturation [37,40]. In addition, it has been reported that the parthenogenetic activation by chemicals is relatively easier in aging oocytes compared to 'fresh' mature oocytes [41]. Interestingly, we found that the significant expression of GPER on oocyte membrane was revealed in the aging oocytes compared with other oocytes, suggesting that the development of GPER on oocyte membrane during maturation may be involved in subsequent oocyte activation process.…”

Section: Discussionmentioning

confidence: 59%

Expression of G protein estrogen receptor (GPER) on membrane of mouse oocytes during maturation

Ren

Zhang

et al. 2013

J Assist Reprod Genet

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Purpose To determine expression of G-protein estrogen receptor (GPER) in mouse oocyte membrane during maturation. Methods The expression of GPER from different maturation stages of oocytes, in vivo and in vitro matured oocytes as well as aging oocytes was examined by immune-fluorescence GPR30 antibody and the images were analyzed by laser scanning confocal microscope. Further confirmation was performed by Western blots for cell fractionation. Results Significant fluorescent signal was observed on the surface of mouse oocytes. The image expression was lower in germinal vesicle (GV) stage than mature metaphase-II (M-II) stage oocytes. There was high expression in in-vivo matured oocytes compared to in vitro matured oocytes. The highest expression was observed in aging oocytes compared with other oocytes. ConclusionsThe changes of expression of GPER on mouse oocytes plasma membrane confirm oocyte membrane maturation, suggesting that those changes of GPER may be related to the functional role of oocyte maturation.

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“…Procedures used for oocyte activation were those reported by Ma et al (2005) with a few modifications. The activating medium used was Ca 2C -free Chatot-Ziomek-Bavister (CZB) medium supplemented with 10 mM SrCl 2 .…”

Section: Oocyte Activation and Embryo Culturementioning

confidence: 99%

Effects of heat stress during in vitro maturation on cytoplasmic versus nuclear components of mouse oocytes

Wang¹,

Sui²,

Miao³

et al. 2009

Reproduction

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The objectives of this study were to investigate the effect of heat stress during in vitro maturation on the developmental potential of mouse oocytes and to determine whether the deleterious effect was on the nuclear or cytoplasmic component. While rates of oocyte nuclear maturation (development to the metaphase II stage) did not differ from 37 to 40 8C, rates for blastocyst formation decreased significantly as maturation temperature increased from 38.5 to 39 8C. Chromosome spindle exchange showed that while blastocyst formation did not differ when spindles matured in vivo or in vitro at 37, 40 or 40.7 8C were transplanted into in vivo matured cytoplasts, no blastocyst formation was observed when in vivo spindles were transferred into the 40 8C cytoplasts. While oocytes reconstructed between 37 8C ooplasts and 37 or 40 8C karyoplasts developed into 4-cell embryos at a similar rate, no oocytes reconstituted between 40 8C ooplasts and 37 8C spindles developed to the 4-cell stage. Immunofluorescence microscopy revealed impaired migration of cortical granules and mitochondria in oocytes matured at 40 8C compared with oocytes matured at 37 8C. A decreased glutathione/GSSG ratio was also observed in oocytes matured at 40 8C. While spindle assembling was normal and no MAD2 was activated in oocytes matured at 37 or 40 8C, spindle assembling was affected and MAD2 was activated in some of the oocytes matured at 40.7 8C. It is concluded that 1) oocyte cytoplasmic maturation is more susceptible to heat stress than nuclear maturation, and 2) cytoplasmic rather than nuclear components determine the pre-implantation developmental capacity of an oocyte.

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Parthenogenetic activation of mouse oocytes by strontium chloride: A search for the best conditions

Cited by 81 publications

References 51 publications

Effects of the conditioned medium of mesenchymal stem cells on mouse oocyte activation and development

Effects of the conditioned medium of mesenchymal stem cells on mouse oocyte activation and development

Expression of G protein estrogen receptor (GPER) on membrane of mouse oocytes during maturation

Effects of heat stress during in vitro maturation on cytoplasmic versus nuclear components of mouse oocytes

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