Soil water content is an important property to indicate the quality of peat in supporting plant growth. Soil nutrient is affected by water content, and each plant has varied adaptation to encounter water stress. This study aimed to observe the effect of water stress to leaf nutrients, nutrient uptake dynamics, and biomass on oil palm seedlings with three different varieties (540, Langkat, Dumpy) and four different water stoppage periods (0, 14, 21, 28 days). Results showed that after 28 days, biomass, leaf nutrients, and plant nutrient uptake (N, P, K, Ca, Mg) from 3 varieties were not statistically different. However, different N, P, K, and Mg uptake only occurred if we compared watering period’s data. The highest water content and biomass correlation were shown in Langkat variety (r = -0.630). The pH increased longest water stoppage period (r = 0.116). Significant correlation was only found in Langkat and Dumpy’s N uptake (r = -0.431 - (- 0.420)), and P uptake of Langkat (r = -0.368). Sensitivity order of nutrient uptake in relation with low water content for 540 variety was N > Ca > Mg > P > K; Langkat variety was N > Ca > Mg > P > K, and Dumpy variety was N > Ca > P > Mg > K.