Pengaruh Pemberian Air Kelapa (Cocos nucifera. L) pada Media Agar Darah terhadap Pertumbuhan Bakteri Mycobacterium tuberculosis

Abstract

The Effect Of Provision Cocos Nucifera L on Blood Agar Plate to Mycobacterium tuberculosis Growth. Tuberculosis is still becoming a health problem in Indonesia. High prevalence of Tuberculosis has encouraged efforts so that patients get treatment immediately. The gold standard to diagnose the disease is by performing bacteriae culture. The culture media for Mycobacterium tuberculosis is quite expensive and also takes quite a long period to let the bacteriae grow up. A blood agar plate is a nonselective media enabling the growth almost all bacteriae. Hopefully, the growth of Mycobacterium tuberculosis becomes more rapid by providing coconut water on a blood agar plate. This study was to provide the effects of provision coconut water with the concentration 50%, 75% and 100% on blood agar plate which were incubated at temperature 35 C and 37 oC. This was an experimental study using pure strain Mycobacterium tuberculosis (H37RV). The result of the study showed that Mycobacterium tuberculosis was able to grow up in a blood agar plate with coconut water 100% concentration which added to the media. Incubation time was 14 days at temperature 35°C and 37°C. Regression logistic test showed a significant value of the concentration, temperature, and duration variable were 0,738, 0,872 and 0,720, respectively. There was no effect of coconut water concentration, temperature dan incubation period to the growth of Mycobacterium tuberculosis.

Isolasi dan Identifikasi Bakteri Gram Positif Staphylococcus aureus dan Micrococcus pada Peternakan Sapi yang Terindikasi Mastitis

“…Suspensi bakteri yang tumbuh dari media NB dipindahkan ke Blood Plate Agar (BPA) untuk mendapatkan koloni terpisah serta untuk melihat kemampuan bakteri dalam menghemolisis sel darah merah (Nuraeni dan Sebayang, 2018;Wahyuningsih dan Zulaika, 2018). Pada penelitian yang dilakukan Isnan et al (2017) Hasil pengamatan makroskopis marfologi koloni bakteri Gram Positif media BPA menunjukkan bahwa langkah identifikasi mikroskopis dengan pewarnaan Gram.…”

Uji Kualitatif Jagung Pulut (Zea mays certaina) sebagai Media Alternatif pertumbuhan Mycobacterium tuberculosis (TB)

“…Penelitian Nuraeni dan Sebayang (2018) membuktikan bahwa Mycobacterium tuberculosis tumbuh pada base agar darah yang ditambahkan air kelapa dengan konsentrasi 100% 6 . Selain itu, mahalnya harga yang di keluarkan untuk pembuatan media instant dalam memenuhi kebutuhan nutrisi yang di perlukan bakteri menjadi kendala yang sangat besar saat ini dalam pengembangan ilmu mikrobiologi.…”