PENGARUH PEMBERIAN EKSTRAK ETANOL JAHE MERAH (Zingiber Officinale Roxb var Rubrum) TERHADAP KUANTITAS DAN KUALITAS SPERMATOZOA MENCIT JANTAN (Mus musculus L.) YANG DIINDUKSI PARAQUAT DIKLORIDA

Abstract

Paraquat is one of the chemicals that contributes to increasing pollution Indonesia. The careless use of paraquat can increase production of ROS (Reactive Oxygen Species), resulting in damage to a wide range of vital organs and reproductive system disorders such as infertility. ROS in the body can be captured by antioxidants. Red Ginger contains high antioxidants because there are active phenolic compounds such as gingerol, shagaol, zingeron, ginggerdiol, and zingibren which are proven to protect body cells from damage caused by ROS. This study was aimed to test the effectiveness of red ginger extract (Zingiber officinale Roxb. Var. Rubrum) on the quantity and quality of sperm in male mice (Mus musculus L.) induced by paraquat dichloride.This research uses a completely randomized design and is divided into six treatment groups with each four replications: K1 as the control group (only given H2O); K2 (paraquat induced a dose of 20 mg/kg Body Weight (BW) without the administration of the test substances); K3; K4; K5 (paraquat induced a dose of 20 mg/kg Body Weight (BW) and was given a red ginger extract at a dose of consecutive succession 6 mg / ml, 12 mg / ml, 18 mg / ml) and K6 (Only given a test material the red ginger extract as much as 18 mg / ml). Paraquat was given 2 times a week for 21 days and the red ginger extract was given for 35 days. The results of analysis by One-way ANOVA followed LSD at 5% significance level showed the ethanolic extract of red ginger can increase the sperm count, motility, viability and Morphology sperm of mice induced by the paraquat diklorida.

Increased levels of Follicle Stimulating Hormone (FSH) and sertoli cells count in wistar rats induced with paraquat after green bean sprout extract supplementation

“…The herbicide paraquat has the effect of increasing free radicals and reactive oxygen species (ROS) in the body, which damages the hypothalamic-pituitary-testicular axis's communication (HHT). Oral administration of paraquat at a dose of 20 mg / Kg BB can diminish the quality of spermatozoa in male rats (Anggraini et al, 2018). In previous studies, paraquat has been shown to reduce spermatozoa quality, whereas in this study paraquat was able to reduce FSH levels and the number of sertoli cells in male Wistar mice, indicating that this study is consistent with previous research.…”

“…The toxicity of paraquat administration orally dose 20 mg/kg BW in male mice, it is possible to reduce sperm quality (Anggraini et al, 2018). Paraquat has the effect of changing the histological structure of the testes and causing spermatogenesis failure in male Wistar rats (Wasiu & Abdulfatai, 2019).…”

Effect of taurine on histopathological features of spermatogenesis in seminiferous tubules of mice (Mus musculus) induced by paraquat

“…In addition, damage to the mitochondrial membrane of sperm cells would reduce the amount of ATP needed for sperm movement, damaged the DNA structure, and led to sperm cell death. Decreased sperm motility could also be caused by sperm maturation in the epididymis (Anggraini et al, 2019).…”

“…Taurine could also stimulate testosterone secretion in both in vivo and in vitro conditions (Yang et al, 2010). Testosterone functioned to control the spermatogenesis process, increased the Sertoli cells, and played a role in determining the quality of spermatozoa (Anggraini et al, 2019). Taurine, which was non-enzymatic, had a positive effect on spermatozoa during cryo conservation due to more minor damage to male gametes.…”