Pengaruh Pemberian Ekstrak Etanol  Umbi Bawang Dayak (Eleutherine palmifolia (L.) Merr.) Secara Oral pada Mencit BALB/c Terhadap Pencegahan Penurunan Diameter Germinal Center pada Kelenjar Getah Bening Serta Kadar IgG Serum

Carmelita, Austin Bertilova

doi:10.20473/jbp.v18i1.2016.1-12

Cited by 7 publications

(7 citation statements)

References 2 publications

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“…Methylprednisolone is a glucocorticoid group compound that can diffuse passively across cell membranes and bind to intracellular glucocorticoid receptors. This methylprednisolone-receptor glucocorticoid complex can bind and block pro-inflammatory agents, increase the expression of anti-inflammatory agents, and inhibit the synthesis of inflammatory cytokines, especially by blocking the function of transcription factors, such as nuclear factor-kappa-B (NF-κB) [9,45,46]. Methylprednisolone includes compounds from corticosteroids that have been commonly used to lower the immune system and are anti-inflammatory.…”

Section: Carbon Clearance Test Results In Balb/c Strain Male Micementioning

confidence: 99%

Immunomodulatory activity of Ananas comosus (L.) Merr. flesh and stem fruit juice on mice with carbon clearance and Staphylococcus aures-induced methods

PURKON,

NURAISYAH,

NAMINA

et al. 2023

jrp

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The body's immune system plays a very important role in defending the body from antigens and infectors that enter the body and strive to eliminate them. Along with increasing public awareness and knowledge, the development of medicines from herbal/natural ingredients is being intensively carried out. Ananas comosus is a type of plant that is widely spread in tropical countries including Indonesia. The fruit is rich in nutrients, vitamins, essential minerals, and the content of the enzyme bromelain which has a wide range of pharmacological activities, including its alleged efficacy as an immunomodulator.This study aims to determine the effective dose of squeezing the flesh and fruit stems of Ananas comosusas an immunomodulator against its phagocytic activity based on the carbon clearance method and the application of its immunomodulator in test animal models induced by Staphylococcus aureus bacteria. The experimental animals used were male mice of the BALB/c strain, which was divided into several groups, namely: normal control group, negative control, immunostimulant positive control(Stimuno Forte ® ), immunosuppressant positive control (methylprednisolone), Ananas comosus fruit flesh and stem squeezing test(ACFSF) dosis 1 (125 mg/kg bw), dose 2 (250 mg/kg bw), and dose 3 (500 mg/kg bw).Examination of these immunomodulatory activity tests, including: therate of carbon elimination velocity, the phagocytosis index, the organ index, and the percentage of phagocytic activity (%).The results of this study have shown that the ACFSF group with the most effective dose as an immunostimulant based on the carbon clearance method and the application of immunomodulators in test animal models induced by S. aureus bacteria is ACFSF dose 3 (500 mg/kg bw) with an index value phagocytos is 1,40 and the largest percentage of phagocytic activity (%) compared to the negative control group and close to the immunostimulant comparison group Stimuno Forte ® .

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Section: Carbon Clearance Test Results In Balb/c Strain Male Micementioning

confidence: 99%

Immunomodulatory activity of Ananas comosus (L.) Merr. flesh and stem fruit juice on mice with carbon clearance and Staphylococcus aures-induced methods

PURKON,

NURAISYAH,

NAMINA

et al. 2023

jrp

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“…The research was conducted by feeding the mice with cow fat to determine the effect towards cholesterol level and triglyceride level in mice blood. Giving the concentration of dayak onion extract refers to the research of Carmelita (2016), with concentrations of 0 mg/Kg BB, 50 mg/Kg BB, 100 mg/Kg BB and 200 mg/Kg BB. Mice are given mixed food consists of CP 511 food concentrate and cow fat using 7:3 ratio of food concentrate: cow fat refers to the research of Shintawati, et al (2011).…”

Section: Methodsmentioning

confidence: 99%

The Effect of Bulb Extract from Dayak Onion (Eleutherine americana Merr) to the Cholesterol Level and the Trigliresida Level in Mice (Mus musculus)

2018

Nusantara Science and Technology Proceedings

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Bawang dayak or dayak onion (Eleutherine americana Merr) is an endemic plant from West Kalimantan. The bulb extract from dayak onion is known to be consumed as traditional medicine by dayak ethnic group. Dayak onion contained alkaloid, flavonoid, glycoside, saponin, and tannin that are very beneficial for health. This research aims to examine the effect of ethanol extract of dayak onion towards the cholesterol level and the triglyceride level in animal testing male mice (Mus musculus). This research uses completely randomized design with experimental laboratory methods by doing 5 treatments and 5 times replications. Mice are given mixed food consists of CP 511 concentrate and cow fat using 7:3 comparison for 10 days. Mice are also given treatment with the ethanol extract of dayak onion for 7 days with classifications of: negative control treatment, positive treatment, 50 mg/Kg BB, 100 mg/Kg BB, and 200 mg/Kg BB. The research result shows that the treatment of ethanol extract dayak onion has significant effect towards the decrease of triglyceride level in mice's blood, but based on LSD test, the differentiation between each treatment is not significant. Based on the research result, ethanol extract of dayak onion according to the average result can indeed decrease the level of cholesterol, but the statistics test has not yet shown significant results. Keywords: ethanol extract of dayak onion, male mice, the level of cholesterol, the level of triglyceride  Corresponding author

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“…Keberadaan flavonoid dapat meningkatkan sekresi IL-12 oleh Antigen-Presenting Cells (APCs) untuk selanjutnya menginduksi sel T (Th-0). Sel Th-0, mengalami proliferasi dan diferensiasi menjadi sel Th-1 dan Th-2 (Carmelita, 2016).…”

Section: Histologi Limpaunclassified

“…Peningkatan aktivitas proliferasi sel limfosit kemudian terjadi yang ditandai dengan adanya perubahan secara mikroskopis berupa peningkatkan luas germinal center dan pulpa putih. Kedua situs ini merupakan lokasi diferensiasi, proliferasi, dan berkumpulnya selsel limfosit (Carmelita, 2016). Hefni et al (2013) menjelaskan bahwa daun kelor selain mengandung senyawa flavonoid, terdapat senyawa saponin dan ion kalsium (Ca) yang mampu menginduksi peningkatan ekspresi IL-2 yang berperan dalam proliferasi sel Th.…”

Section: Histologi Limpaunclassified

Potensi Imunomodulator Herbal Ekstrak Etanol Daun Pepaya Varietas Calina terhadap Struktur Jaringan Limpa Tikus Putih Galur Wistar

Setiawan¹,

Wulandari²,

Fitriyani³

2021

jveteriner

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Tanaman pepaya varietas calina (Carica papaya L. Var. calina) merupakan salah satu jenis tanaman pepaya yang banyak dibudidayakan di Indonesia. Tanaman ini memiliki kandungan senyawa aktif yang dapat berperan dalam meningkatkan kerja imun seperti flavonoid, polifenol, tanin dan saponin. Penelitian ini bertujuan untuk mengetahui aktivitas ekstrak etanol daun pepaya varietas calina sebagai imunomodulator alami terhadap struktur jaringan limpa tikus putih galur wistar (Rattus norvegicus Berkenhout, 1769). Penelitian menggunakan 24 ekor tikus wistar yang dibagi ke dalam empat perlakuan pemberian ekstrak yaitu kontrol (dosis 0 mg/kg BB), P1 (dosis 100 mg/kg BB), P2 (dosis 200 mg/kg BB), dan dosis P3 (300 mg/kg BB) selama 30 hari per oral. Parameter yang diamati meliputi bobot badan, rasio bobot limpa dan histologi limpa yang terdiri dari luas dan diameter pulpa putih serta luas germinal center. Tikus dikorbankan nyawanya pada hari ke-31, kemudian organ limpa diambil dan difiksasi dengan neutral buffer formaline 10%. Limpa dibuat sediaan histologis dengan menggunakan blok parafin dan pewarnaan hematoxylin-eosin. Data dianalisis dengan menggunakan sidik ragam satu arah kemudian dilanjutkan uji jarak berganda Duncan untuk mengetahui tingkat signifikansi antar perlakuan. Hasil penelitian menunjukkan bahwa seluruh perlakuan memberikan pengaruh yang signifikan (P<0,05), dalam hal menambah diameter dan memperluas pulpa putih serta memperluas germinal center pada limpa dibandingkan dengan kontrol. Bobot badan dan rasio organ limpa tidak menunjukan perbedaan secara siginifikan antar perlakuan (P>0,05). Simpulan penelitian adalah ekstrak etanol daun pepaya varietas calina memiliki potensi sebagai agen imunomodulator alami.

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Pengaruh Pemberian Ekstrak Etanol Umbi Bawang Dayak (Eleutherine palmifolia (L.) Merr.) Secara Oral pada Mencit BALB/c Terhadap Pencegahan Penurunan Diameter Germinal Center pada Kelenjar Getah Bening Serta Kadar IgG Serum

Cited by 7 publications

References 2 publications

Immunomodulatory activity of Ananas comosus (L.) Merr. flesh and stem fruit juice on mice with carbon clearance and Staphylococcus aures-induced methods

Immunomodulatory activity of Ananas comosus (L.) Merr. flesh and stem fruit juice on mice with carbon clearance and Staphylococcus aures-induced methods

The Effect of Bulb Extract from Dayak Onion (Eleutherine americana Merr) to the Cholesterol Level and the Trigliresida Level in Mice (Mus musculus)

Potensi Imunomodulator Herbal Ekstrak Etanol Daun Pepaya Varietas Calina terhadap Struktur Jaringan Limpa Tikus Putih Galur Wistar

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