PENGARUH PEMBERIAN EKSTRAK KULIT SEMANGKA (Citrullus lanatus) TERHADAP MOTILITAS DAN VIABILITAS SPERMATOZOA TIKUS (Rattus norvegicus) DENGAN PAPARAN SUHU PANAS

Abstract

High temperatures lead to oxidative stress which can decrease the quality of spermatozoa. Watermelon’s rind have an ability for antioxidants effect that can reduce sperm’s damages that results by role explanation of free radicals. This study aimed to determine the effect of extract of watermelon’s rind on the motility and viability of sperm on male rat exposed to heat. This experiments use rat as animals experiment with five treatments and four repetitions. Five treatments are: P0: without exposured to 40˚C of temperature and without treatment by extract watermelon’s rind, P1: was exposured to 40˚C of temperature and without treatment by extract watermelon’s rind, P2: was exposured to 40˚C of temperature and treatments by extract watermelon’s rind dosage 20 mg/rat/day, P3: was exposured to 40˚C of temperature and treatments by extract watermelon’s rind dosage 40 mg/rat/day, and P4: was exposured to 40˚C of temperature and treatments by extract watermelon’s rind dosage 80 mg/rat/day. The treatment was done for 52 days. The results, P4 have the best effect that can be increase the motility and viability of rat’s sperm (Rattus norvegicus) exposed to heat.

THE EFFECT OF WATERMELON (Citrullus lanatus) RIND ETHANOLIC EXTRACT ON THE NUMBER OF LEYDIG, SERTOLI, AND SPERMATOGENIC CELLS OF RAT (Rattus novergicus) EXPOSED TO HEAT

“…247/HRECC.FORM/IX/2018. Heat exposure was performed in a 50 x 30 x 40 cm chamber with heating lamp and thermostat to keep the temperature at a steady 40°C as earlier study (Rohmah et al, 2018).…”

THE EFFECT OF RED DRAGON FRUIT SKIN EXTRACT (Hylocereus polyrhizus) ON THE NUMBER OF LEYDIG CELLS, DIAMETER OF SEMINIFEROUS TUBULES, AND TESTICULAR WEIGHT OF MALE MICE (Mus musculus) EXPOSURED WITH HOT TEMPERATURES

“…Meanwhile, the treatment groups mice were exposed to heat (40°C) for 45 minutes daily and administered with placebo, RDFPE dose of 250, 500, and 1000mg/kg BW respectively for T0, T1, T2, and T3, for 36 days. Heat exposure was conducted in a 50 x 30 x 40 cm chamber with heat derived from two, 5 Watts light bulbs and a thermostat to keep the temperature steady at 40°C (Rohmah et al, 2018). The RDFPE was administered 0.5 ml per mouse orally through at a stomach tube.…”

Pengaruh pemberian ekstrak daun kelor (Moringa oleifera Lam.) terhadap motilitas dan viabilitas spermatozoa tikus (Rattus norvegicus) dengan paparan panas

“…Pemberian larutan CMC-Na 1% dan larutan EDK dilakukan secara per oral sebanyak 1 ml menggunakan sonde lambung, di pagi hari sebelum makan. Pemaparan panas dilakukan pada bilik berukuran 50 x 30 x 40 cm dengan lampu pemanas dan termostat untuk menjaga suhu tetap stabil 40 °C (Rohmah et al, 2018).…”

“…Perlakuan dilakukan selama 14 hari dan pada hari ke-22 pemeliharaan, semua tikus dikorbankan dengan cara dieutanasia dengan ketamine dan xylazin kemudian dilakukan pembedahan skrotum untuk mengambil cauda epididymis. Pada posisi 1 cm di bawah korda spermatika diklem, kemudian dipotong dan dikeluarkan spermatozoa dengan penekanan dan pendorongan pada bagian cauda epididymis, ditampung pada cawan petri berukuran 3,5 cm (Nunc™, Thermo Scientific) yang berisi 1 ml NaCL 0,9% dan diaduk agar menjadi homogen (Rohmah et al, 2018).…”