This chemistry literacy assessment instrument developed because of recent challenge toward student learning achievement evaluation so it could not only grade studentâs cognitive ability in term of understanding and memorizing, but also will grade the application of studentsâ concept to face any problems. The availability of the chemistry literacy assessment instrument consists of various aspects, such as content, context, High Order Learning Skills (HOLS), and attitude. These aspects are expected to answer those challenges. This study aims to produce a proper and right chemistry literacy assessment toward fundamental law of chemistry and stoichiometry topic based on content validity value and questions numbers, reliability grade, difficulty level and chemistry literacy question differentiator level. This study is the development type, which is Model of Educational Reconstruction (MER) design. MER consists of three stages, (1) analysis of content structure, (2) empirical study, (3) development of instruction evaluation. The clarification of a test instrument on empirical study was done toward three chemistry and education experts (lecturer and teacher). The instrument used was chemistry literacy question which consists of seven discourse question, then expanded into 15 questions. The result showed that content validity value of the designed chemistry literacy assessment could be categorized as valid with 1,11 mark. From 15 questions, there are three questions in âvery significantâ category, nine in âsignificantâ category and three other in âinsignificantâ category. The reliability test is 0,88. In difficulty level, there are eight questions in âmediumâ category, six in âdifficultâ and one in âvery difficultâ. The differentiator level, there are nine questions in âmediumâ category, four in âproperâ and two other in ânot properâ. Question in âinsignificantâ and ânot properâ category is rejected. In conclusion, from 15 designed questions, there are 12 questions in âproperâ and ârightâ category.