This study aimed to determine the effect of the length of storage and soaking of coconut water growth regulators on TSS shallot seedlings. The research was conducted in Torongrejo Village, Junrejo District, Batu City, from April to May 2023. The experiment used a simple randomised block design (RBD) with the following treatments: The first factor is the freshness of young coconut water consisting of 4 levels, namely S0 = fresh/not stored young coconut water, S2 = young coconut water stored two days at room temperature and S3 = young coconut water stored three days at room temperature. Factor II was the soaking time for TSS consisting of 3 levels, namely P1 = TSS soaked for 1 hour, P2 = TSS soaked for 2 hours, and P3 = TSS soaked for 3 hours. The results showed that coconut water’s natural growth regulators contain many substances that help plant growth, including the hormones auxin, cytokinins, and gibberellins, so their effectiveness depends on the length of time the coconut water has been soaked and the coconut water’s shelf life. If the coconut water is stored longer, the soaking time will be shorter, namely 1 hour, and if the coconut water is stored, the time required is shorter.