Peningkatan performans pedet sapi Peranakan Ongole pasca sapih melalui perbaikan manajemen dengan pemanfaatan sumberdaya lokal

Utomo, Bagus Sediadi Bandol; Oelviani, Renie; Subiharta, Subiharta

doi:10.13057/psnmbi/m010430

Cited by 4 publications

(3 citation statements)

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“…Ongole grade cattle is the result of an uncontrolled mating and a grading up of Java and Sumba Ongole cattle (Suyadi et al, 2014, Hardosubroto, 1994. In Kebumen, Central Java, PO cattle genetic improvement program have been done before 1930s (Utomo et al, 2015). Kebumen also become as one of the breeding center of PO cattle (DGLSAH, 2015).…”

Section: Introductionmentioning

confidence: 99%

Identification of MC4R gene and its association with body weight and body size in Kebumen Ongole Grade cattle

Maharani

Fathoni

Sumadi

et al. 2018

J. Indonesian Trop. Anim. Agric.

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ABSTRAKGen MC4R dikenal sebagai kandidat gen untuk sifat pertumbuhan. Penelitian ini bertujuan untuk mengidentifikasi gen MC4R pada sapi Peranakan Ongole (PO) Kebumen dan hubungannya terhadap sifat pertumbuhan ternak tersebut. Data bobot lahir (BL), bobot sapih (BS), panjang badan lahir (PBL), lingkar dada lahir (LDL), tinggi gumba (TG), panjang badan sapih (PBS), lingkar dada sapih (LDS), tinggi gumba sapih (TGS) dan rata-rata pertumbuhan bobot badan harian (ADG) digunakan untuk analisis asosiasi berdasarkan gen MC4R. Total 60 sampel darah diambil dan digunakan untuk isolasi DNA.Produk PCR diamplifikasi dengan menggunakan primer forward 5'-GTCGGGCGTCTTGTTCATC-3' dan reverse 5' GCTTGTGTTTAGCATCGCGT-3'. Single Nucleotide Polymorphisms (SNP) g.1133 C>G digunakan untuk proses genotyping menggunakan metode PCR-RFLP dengan enzim restriksi HpyCH4IV. Frekuensi alel G (0,59) lebih besar dibandingkan dengan alel C (0.41). Frekuensi genotip tertinggi dimiliki oleh ternak bergenotip heterozigot CG, diikuti dengan ternak bergenotipe homozigot GG (0,33) dan CC (0,15). Hasil uji Chi-square menunjukkan populasi tidak menyimpang (P>0,05) dari Hardy-Weinberg Equilibrium (HWE). Hasil analisis asosiasi menunjukkan bahwa SNP g. 1133 C>G pada gen MC4R berpengaruh nyata terhadap panjang badan lahir ternak dengan genotip GG (P<0,05). Kesimpulannya, SNP g.1133 C>G dapat digunakan sebagai marker untuk seleksi sapi PO Kebumen berdasarkan sifat panjang badan saat lahir.Kata kunci: sapi PO Kebumen, Gen MC4R, bobot badan, ukuran tubuh ABSTRACT MC4R gene is known as an important candidate gene for the growth trait. The purpose of this research was to identify the MC4R gene in Kebumen Ongole grade cattle and examine its association with growth traits. Data of birth weight (BW), weaning weight (WW), birth body length (BBL), birth chest circumference (BCC), birth shoulder height (BSH), weaning body length (WBL), weaning chest circumference (WCC), weaning shoulder height (WSH) and average daily gain (ADG) were collected and used for analysis of MC4R gene. Sixty blood samples were collected for DNA isolation and PCR amplification. The single nucleotide polymorphisms (SNP) g.1133 C>G was used for genotyping by using PCR-RFLP methods. The frequenciy of G allele (0.59) was greater than C allele (0.41). The highest genotype frequencies have been detected in CG heterozygote animals (0.52) followed by GG (0.33) and CC (0.15) in homozygote animals. The results of Pearson 's Chi-square test indicated that the population was not deviate (P>0.05) from the Hardy-Weinberg equilibrium (HWE). The SNP g. 1133 C>G of MC4R gene indicated affecting high birth body length with GG genotype (P<0.05). In Kebumen Ongole Grade Cattle (D. Maharani et al.) 87 conclusion, the SNP g. 1133 C>G may can be a marker for birth body length of calf selection. Identification of MC4R Gene in

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Section: Introductionmentioning

confidence: 99%

Identification of MC4R gene and its association with body weight and body size in Kebumen Ongole Grade cattle

Maharani

Fathoni

Sumadi

et al. 2018

J. Indonesian Trop. Anim. Agric.

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“…In Kebumen, breeding programe to improve genetic quality of PO cattle had been done before 1930 and they look like a pure Ongole cattle (Utomo et al, 2015). Based on physical observation in the field, we found the similarities between Kebumen Ongole grade cattle and Brahman cattle.…”

Section: Introductionmentioning

confidence: 77%

Haplotype Diversity of Partial Cytochrome b Gene in Kebumen Ongole Grade Cattle.

Hartatik

Maharani

Sidadolog

et al. 2018

Trop. Anim. Sci. J.

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Cytochrome b gene is commonly used to figure out the genetic variation in animals. This research was designed to explore specific markers of Kebumen Ongole grade cattle and to understand the relationship between Kebumen and Brahman cattle using mtDNA cyt b sequences. The sequence of mtDNA cytochrome b gene of 14 individuals Kebumen Ongole grade cattle and 15 individuals Brahman cattle was determined by using polymerase chain reaction and sequencing with a pair of primer. The haploview software was utilized to linkage disequilibrium (LD) analysis. As a result, 36 SNPs (single nucleotide polymorphisms) with 8 haplotypes were identified in Kebumen Ongole grade cattle. In contrast, a small number of SNPs (7) and haplotypes (6) were observed in Brahman cattle. The homozigosity in Brahman cattle was higher than that in Kebumen Ongole grade cattle cattle. Moreover, based on mtDNA sequences, 50% of Kebumen Ongole grade cattle had 85% similarity with Brahman cattle. The SNP number and LD values of Kebumen Ongole grade cattle were greater than those of Brahman cattle. It was presumed that Brahman cattle might be introduced into Kebumen territory. In conclusion, some of Kebumen Ongole grade cattle have genetic relation with Brahman cattle. In addition, the information of SNP and haplotype results from this research can be used as markers to differentiate breeds of animals.

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“…So then, almost about 90% of the Ongole Grade cattle population in Indonesia were on Java Island. Previous researchers reported that in Kebumen (Central Java), long before the Ongolisation program in the 1930s, efforts had been made to genetically improve zebu cattle originating from Madras (East India) and have continued to grow, so that recently Ongole Grade cattle in Kebumen were also called as Madras cattle and reported had quality like pure Ongole cattle (Utomo et al 2015).…”

Section: Introductionmentioning

confidence: 99%

Genetic diversity and phylogenetic study of Ongole Grade cattle population in Central Java based on blood protein polymorphism

et al. 2023

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Abstract. Lestari DA, Sutopo, Setiaji A, Kurnianto E. 2023. Genetic diversity and phylogenetic study of Ongole Grade cattle population in Central Java based on blood protein polymorphism. Biodiversitas 24: 617-624. This research provides useful information to reflect the genetic structure and to determine appropriate breeding strategies for the Ongole Grade cattle population in Central Java. The objectives of this research were to investigate the genetic diversity and phylogenetic relationship of the Ongole Grade cattle (Bos indicus) population in Central Java based on blood protein polymorphism. A total of 208 blood samples were collected, which belonged to 6 populations of Ongole Grade cattle in Central Java, namely Rembang, Blora, Kebumen, Semarang, Magelang, and Kudus regency. Blood protein analysis was conducted by Sodium Dodecyl Sulfate-Polyacrilamide Gel Electrophoresis (SDS-PAGE) methods to separate blood protein based on molecule weight. Results showed 4 blood protein locus were clearly identified, namely Albumin (Alb), Post Albumin (Pa), Ceruloplasmin (Cp), and Amylase-I (Amy-I), which formed 9 identified alleles and 15 genotypes. All populations had different allele and gene frequency distribution for each locus, which dominated by allele AlbB (0.525), PaB (0.556), CpF (0.608), and Amy-IB (0.639) and by genotype BB (0.312) for Alb loci, BB (0.429) for Pa loci, FS (0.423) for Cp loci and BB (0.472) for Amy-I. Ongole Grade cattle population in this study showed deviation in Hardy-Weinberg Equilibrium (HWE) (P<0.05) for Blora, Kebumen, Semarang, and Kudus, while the rest populations showed in HWE condition. Average heterozygosity and genetic distance of the Ongole Grade cattle population in the recent study were range 0.328-0.529 (for Magelang and Kebumen populations, respectively) and 0.0057-0.0955 (for the Rembang-Kebumen population and Magelang-Kebumen population), respectively. In conclusion, Ongole Grade cattle population in Central Java had high genetic diversity and was divided into two major clusters.

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Peningkatan performans pedet sapi Peranakan Ongole pasca sapih melalui perbaikan manajemen dengan pemanfaatan sumberdaya lokal

Cited by 4 publications

References 1 publication

Identification of MC4R gene and its association with body weight and body size in Kebumen Ongole Grade cattle

Identification of MC4R gene and its association with body weight and body size in Kebumen Ongole Grade cattle

Haplotype Diversity of Partial Cytochrome b Gene in Kebumen Ongole Grade Cattle.

Genetic diversity and phylogenetic study of Ongole Grade cattle population in Central Java based on blood protein polymorphism

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