Perfusion in hamster skin treated with glycerol

Zaman, Raiyan T.; Parthasarathy, Anand; Vargas, Gracie; Chen, Bin; Dunn, Andrew K.; Rylander, H. G.; Welch, Ashley J.

doi:10.1002/lsm.20803

Cited by 8 publications

(8 citation statements)

References 24 publications

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“…SLS‐induced elevation in RBCV was successfully moderated by 10% glycerol and both applied concentrations of xylitol. Glycerol may directly affect microcirculation: it has been reported that 100% anhydrous glycerol reduces blood flow velocity and vessel diameter of micro‐vasculatures . As glycerol we used was one magnitude lower than that in the mentioned study and application of polyols decreased not only dermal blood flow but also other indicators of inflammation, we presume that in this examination, decrease in RBCV in polyol‐treated groups seems to originate in the anti‐inflammatory activity of these compounds.…”

Section: Discussionmentioning

confidence: 60%

Anti‐irritant and anti‐inflammatory effects of glycerol and xylitol in sodium lauryl sulphate‐induced acute irritation

Szél

Polyánka

Szabó

et al. 2015

Acad Dermatol Venereol

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Background Glycerol is known to possess anti‐irritant and hydrating properties and previous studies suggested that xylitol may also have similar effects. Objective Our aim was to study whether different concentrations of these polyols restore skin barrier function and soothe inflammation in sodium lauryl sulphate (SLS)‐induced acute irritation. Methods The experiments were performed on male SKH‐1 hairless mice. The skin of the dorsal region was exposed to SLS (5%) for 3 h alone or together with 5% or 10% of glycerol respectively. Further two groups received xylitol solutions (8.26% and 16.52% respectively) using the same osmolarities, which were equivalent to those of the glycerol treatments. The control group was treated with purified water. Transepidermal water loss (TEWL) and skin hydration were determined. Microcirculatory parameters of inflammation were observed by means of intravital videomicroscopy (IVM). Furthermore, accumulation of neutrophil granulocytes and lymphocytes, the expression of inflammatory cytokines and SLS penetration were assessed, as well. Results Treatment with the 10% of glycerol and both concentrations of xylitol inhibited the SLS‐induced elevation of TEWL and moderated the irritant‐induced increase in dermal blood flow and in the number of leucocyte‐endothelial interactions. All concentrations of the applied polyols improved hydration and prevented the accumulation of lymphocytes near the treatment site. At the mRNA level, neither glycerol nor xylitol influenced the expression of interleukin‐1 alpha. However, expression of interleukin‐1 beta was significantly decreased by the 10% glycerol treatment, while expression of tumour necrosis factor‐alpha decreased upon the same treatment, as well as in response to xylitol. Higher polyol treatments decreased the SLS penetration to the deeper layers of the stratum corneum. Conclusion Both of the analysed polyols exert considerable anti‐irritant and anti‐inflammatory properties, but the effective concentration of xylitol is lower than that of glycerol.

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Section: Discussionmentioning

confidence: 60%

Anti‐irritant and anti‐inflammatory effects of glycerol and xylitol in sodium lauryl sulphate‐induced acute irritation

Szél

Polyánka

Szabó

et al. 2015

Acad Dermatol Venereol

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“…At the higher concentration of 90% v/v glycerin, the main vessels of the CAM were damaged by blockage or coagulation [26] . With pure glycerin, an increase in viscosity of blood and poor recovery were observed [27] . Glycerin was reported to cause embryotoxicity [4] .…”

Section: Discussionmentioning

confidence: 99%

The chick chorioallantoic membrane imaging method as a platform to evaluate vasoactivity and assess irritancy of compounds

Tay

Heng

Chan

2012

Journal of Pharmacy and Pharmacology

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“…Previous optical clearing studies in skin have documented a decrease in blood flow and even closure of blood vessels, 15,21 followed by vessel recovery as the concentration of hyperosmotic agent dissipates. These studies have proposed that dehydration and hydration of the hamster dorsal skin by the application of a hyperosmotic agent and 0.9% saline, respectively, are primarily responsible for reducing light scattering observed in optical clearing.…”

Section: Introductionmentioning

confidence: 98%

“…14 However, glycerol causes a decrease in blood flow when this agent is used in skin. 15 Thus, these experiments are designed to examine the dynamics of blood volume fraction and mean blood vessel diameter in the conjunctiva and choroid after the application of glycerol using diffuse optical spectroscopy (DOS) measurements.…”

Section: Introductionmentioning

confidence: 99%

Changes in morphology and optical properties of sclera and choroidal layers due to hyperosmotic agent

et al. 2011

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Light scattering in the normally white sclera prevents diagnostic imaging or delivery of a focused laser beam to a target in the underlying choroid layer. In this study, we examine optical clearing of the sclera and changes in blood flow resulting from the application of glycerol to the sclera of rabbits. Recovery dynamics are monitored after the application of saline. The speed of clearing for injection delivery is compared to the direct application of glycerol through an incision in the conjunctiva. Although, the same volume of glycerol was applied, the sclera cleared much faster (5 to 10 s) with the topical application of glycerol compared to the injection method (3 min). In addition, the direct topical application of glycerol spreads over a larger area in the sclera than the latter method. A diffuse optical spectroscopy system provided spectral analysis of the remitted light every two minutes during clearing and rehydration. Comparison of measurements to those obtained from phantoms with various absorption and scattering properties provided estimates of the absorption coefficient and reduced scattering coefficient of rabbit eye tissue.

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Perfusion in hamster skin treated with glycerol

Abstract: Blood flow velocity and vessel diameter of the micro-vasculatures of hamster skin were reduced by the application of 100% anhydrous glycerol. At 24 hours, capillary perfusion remained depressed.

Cited by 8 publications

References 24 publications

Anti‐irritant and anti‐inflammatory effects of glycerol and xylitol in sodium lauryl sulphate‐induced acute irritation

Anti‐irritant and anti‐inflammatory effects of glycerol and xylitol in sodium lauryl sulphate‐induced acute irritation

The chick chorioallantoic membrane imaging method as a platform to evaluate vasoactivity and assess irritancy of compounds

Changes in morphology and optical properties of sclera and choroidal layers due to hyperosmotic agent

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