Pharmacological inhibition of gelatinase B induction and tumor cell invasion

McMillan, James I.; Weeks, Reitha S.; West, James W.; Bursten, Stuart L.; Rice, Glenn C.; Lovett, David H.

doi:10.1002/(sici)1097-0215(19960807)67:4<523::aid-ijc11>3.0.co;2-8

Cited by 30 publications

(5 citation statements)

References 24 publications

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“…25 Especially, MMP-9 (gelatinase B, 92 kDa type IV collagenase) has been suggested to play an important role in cell migration by facilitating the destruction of type IV collagen-containing basement membrane that separates the epithelial and stromal compartments. 26 To identify the active compounds responsible for inhibitory effect on MMP-9 and MCP-1 of rosemary, the activities of MMP-9 secreted from VSMCs were measured with RM and its fractions (10 lg/mL). RM and RH significantly decreased MMP-9 secretion compared to the other fractions, as ascertained using a gelatin zymogaphy assay (data not shown).…”

Section: Resultsmentioning

confidence: 99%

Effect ofRosemarinus officinalisL. on MMP-9, MCP-1 Levels, and Cell Migration in RAW 264.7 and Smooth Muscle Cells

Chae

et al. 2012

Journal of Medicinal Food

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Atherosclerosis is a chronic and progressive inflammatory disease. Novel anti-inflammatory therapies may have promise as treatment strategies for cardiovascular risk reduction. Rosemary (Rosemarinus officinalis L.) has been used in folk medicine to treat headaches, epilepsy, poor circulation, and many other ailments. It was found that rosemary could act as a stimulant and mild analgesic and could reduce inflammation. However, the mechanisms underlying the anti-inflammatory and antiatherosclerotic effects of rosemary need more study. This study investigated effects of the rosemary components, carnosic acid (CA), and carnosol (CAR), on cell migration. Monocyte chemoattractant protein-1 (MCP-1) and matrix metalloproteinase-9 (MMP-9) were determined by Western blot and gelatin zymography, respectively, in RAW 264.7 macrophages and vascular smooth muscle cells (VSMCs). VSMC migration was assessed by a Matrigel migration assay. Active compounds of rosemary extracts were also analyzed using a reversed-phase high-performance liquid chromatography. MMP-9 and MCP-1 activities were markedly diminished with methanol extract (RM), n-hexane fraction (RH), and CA in RAW 264.7 cells. RM, RH, CA, and CAR suppressed tumor necrosis factor-alpha-induced VSMC migration by inhibiting MMP-9 expression. Chromatograms of RM-and RH-containing CA and CAR revealed higher CA contents of RM (9.4%, 93.85 lg/mg dry wt.) and, especially, RH (18.4%, 184.00 lg/mg dry wt.), which were appreciably elevated compared with the similar CAR content in RM and RH (3.7%, 37.30 lg/mg dry wt.; and 2.5%, 25.05 lg/mg dry wt., respectively). Rosemary, especially its CA component, has potential antiatherosclerosis effects related to cell migration.KEY WORDS: Rosemarinus officinalis L. rosemary matrix metalloproteinase-9 (MMP-9) monocyte chemoattractant protein-1 (MCP-1) migration RAW 264.7 macrophages vascular smooth muscle cell

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Section: Resultsmentioning

confidence: 99%

Effect ofRosemarinus officinalisL. on MMP-9, MCP-1 Levels, and Cell Migration in RAW 264.7 and Smooth Muscle Cells

Chae

et al. 2012

Journal of Medicinal Food

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“…MMP-9 can degrade the type IV collagen-containing basement membrane that separates the epithelial and stromal compartments. 85 In addition, MMP-9 can activate several cytokines such as latent transforming growth factor- β and pro-TNF- α to their active forms. 84,86 Thus, the reduced expression of MMP-9 in peritoneal macrophages not only decreases macrophage scavenger activity but also attenuates the immune system's defensive mechanism.…”

Section: Suppression Of Phagocytosismentioning

confidence: 99%

Prostaglandin E₂: the master of endometriosis?

Wu¹,

Chuang

et al. 2010

Exp Biol Med (Maywood)

124

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Endometriosis is the primary cause of infertility in women, with a prevalence rate ranging from 5% to 10%. Women with endometriosis suffer from symptoms such as chronic pelvic pain, dysmenorrhea and dyspareunia, which significantly reduce the quality of life. Endometriosis is a polygenic disease with a complex, multifactorial etiology. The mechanism responsible for the initiation and development of this disease remains largely unknown. Prostaglandin E(2) (PGE(2)), a versatile eicosanoid that exerts numerous physiological and pathological functions, has been implicated to play critical roles in the development of endometriosis. A growing body of evidence demonstrates that PGE(2) regulates many pathophysiological processes including cell proliferation, antiapoptosis, immune suppression and angiogenesis during the development of endometriosis. This review focuses on recent advances in cellular and molecular mechanisms triggered by PGE(2) that contribute to the pathological processes of endometriosis.

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“…12,20 -22 In addition, MMP-9 can facilitate the destruction of the type IV collagen-containing basement membrane, which separates the epithelial and stromal compartment. 23 We hypothesize that the decreased phagocytotic capability of peritoneal macrophages in women with endometriosis may be due to inhibition of MMP-9 expression and activity by unidentified factors in the peritoneal fluid (PF) of women with endometriosis. In this study, we aim to investigate the expression level and enzymatic activity of MMP-9 secreted by peritoneal macrophages derived from normal women and women with endometriosis.…”

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confidence: 99%

Suppression of Matrix Metalloproteinase-9 by Prostaglandin E2 in Peritoneal Macrophage Is Associated with Severity of Endometriosis

Shoji

et al. 2005

The American Journal of Pathology

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Decreased phagocytotic ability of macrophages has been reported to be associated with the severity of endometriosis, although the underlying mechanism remains uncharacterized. Expression and secretion of matrix metalloproteinase (MMP)-9 by macrophages is a means to degrade the extracellular matrix of cells that are designated for phagocytosis. Here, we describe the regulation of MMP-9 expression and activity in peritoneal macrophages of women with endometriosis. Results demonstrated that peritoneal macrophages isolated from women with endometriosis have decreased levels of protein and enzyme activity of MMP-9. Treatment of macrophages with peritoneal fluid obtained from patients with severe endometriosis inhibited MMP-9 expression and gelatinase activity. Further investigation identified prostaglandin (PG) E 2 as the major factor in the peritoneal fluid that inhibited MMP-9 activity. The inhibitory effect of PGE 2 was mediated via the EP2/EP4-dependent PKA pathway. Furthermore, expression of tissue inhibitor of metalloproteinase-1, tissue inhibitor of metalloproteinase-2, and RECK in macrophages was not affected by treatment with PGE 2 , indicating the effect of PGE 2 on suppressing MMP-9 activity was not mediated by up-regulation of its inhibitor. Our results suggest that decreased phagocytotic capability of peritoneal macrophage in patients with endometriosis may be caused by PGE 2 -mediated decreases in MMP-9 expression. Endometriosis is a common gynecological disorder with a complex, multifactorial etiology that causes chronic pelvic pain, dysmenorrhea, and even infertility. The prevalence of this disease is ϳ10 to 15% among women of reproductive age. The underlying pathophysiological mechanism is still enigmatic. Although retrograde menstruation has been suggested to be the crucial constituent in the development of endometriosis, 1 factors allowing the implantation and propagation of endometriotic lesions are primarily unclear. Aberrant production of steroids by ectopic endometriotic lesions and alteration/ dysfunction of the immune system may lead to the development of endometriosis.2-5 During the development of endometriosis, immune cells are recruited into the peritoneal cavity. Among these immune cells, macrophages are the dominant cell type in the peritoneal cavity and are involved in phagocytosis and inflammation, especially in cleaning the retrograded endometrial debris.6,7 Peritoneal macrophages isolated from patients with endometriosis were found to have phenotypic and functional alterations leading to poor phagocytotic capacity, which is highly associated with severity of endometriosis. 4,8 Nevertheless, the mechanism of suppressed phagocytotic capability of macrophages in endometriosis is poorly understood.Matrix metalloproteinases (MMPs), also called matrixins, are proteinases that participate in extracellular matrix degradation.9 Based on substrate specificity, sequence similarity, and domain organization, vertebrate MMPs can be divided into six groups such as collagenases, gelatinases, stromel...

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Pharmacological inhibition of gelatinase B induction and tumor cell invasion

Cited by 30 publications

References 24 publications

Effect ofRosemarinus officinalisL. on MMP-9, MCP-1 Levels, and Cell Migration in RAW 264.7 and Smooth Muscle Cells

Effect ofRosemarinus officinalisL. on MMP-9, MCP-1 Levels, and Cell Migration in RAW 264.7 and Smooth Muscle Cells

Prostaglandin E₂: the master of endometriosis?

Suppression of Matrix Metalloproteinase-9 by Prostaglandin E2 in Peritoneal Macrophage Is Associated with Severity of Endometriosis

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Pharmacological inhibition of gelatinase B induction and tumor cell invasion

Cited by 30 publications

References 24 publications

Effect ofRosemarinus officinalisL. on MMP-9, MCP-1 Levels, and Cell Migration in RAW 264.7 and Smooth Muscle Cells

Effect ofRosemarinus officinalisL. on MMP-9, MCP-1 Levels, and Cell Migration in RAW 264.7 and Smooth Muscle Cells

Prostaglandin E2: the master of endometriosis?

Suppression of Matrix Metalloproteinase-9 by Prostaglandin E2 in Peritoneal Macrophage Is Associated with Severity of Endometriosis

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Prostaglandin E₂: the master of endometriosis?