Phosphate Solubilizing Microorganism Bacillus sp. MVY-004 and Its Significance for Biomineral Fertilizers’ Development in Agrobiotechnology

Abstract

In this study, a phosphate solubilizing microorganism was isolated from the soil of an agricultural field in Lithuania. Based on 16S rRNA gene sequence analysis, the strain was identified as Bacillus sp. and submitted to the NCBI database, Sector of Applied Bio-catalysis, University Institute of Biotechnology, Vilnius, Lithuania and allocated the accession number KY882273. The Bacillus sp. was assigned with the number MVY-004. The culture nutrient medium and growth conditions were optimized: molasses was used as a carbon source; yeast extract powder was used as an organic source; NH4H2PO4 was used as a nitrogen source; the culture growth temperature was 30 ± 0.5 °C; the initial value of pH was 7.0 ± 0.5; the partial pressure of oxygen (pO2) was 60 ± 2.0; the mixer revolutions per minute (RPM) were 25–850, and the incubation and the fermentation time was 48–50 h. Analysis using Liquid Chromatography Time-of-Flight Mass Spectrometry (LC-TOF/MS) results showed that Bacillus sp. MVY-004 produced organic acids such as citric, succinic, 2-ketogluconic, gluconic, malic, lactic, and oxalic acids. Furthermore, the experiment showed that Bacillus sp. MVY-004 can also produce the following phytohormones: indole-3-acetic (IAA), jasmonic (JA), and gibberellic (GA3) acids. In the climate chamber, the experiment was performed using mineral fertilizer (NPS-12:40:10 80 Kg ha−1) and mineral fertilizers in combination with Bacillus sp. MVY-004 cells (NPS-12:40:10 80 Kg ha−1 + Bacillus sp. MVY-004) in loamy soil. Analysis was performed in three climate conditions: normal (T = 20 °C; relative humidity 60%); hot and dry (T = 30 °C; relative humidity 30%); hot and humid (T = 30 °C; relative humidity 80%).

Phosphate-Solubilizing Bacteria Isolated from Phosphate Solid Sludge and Their Ability to Solubilize Three Inorganic Phosphate Forms: Calcium, Iron, and Aluminum Phosphates

“…Many PSB strains have been observed secreting a variety of organic acids, including acetic, citric, formic, oxalic, and formic acid, amongst many others. Organic acids [ 1 , 25 , 37 , 38 , 39 , 40 , 41 ] convert tricalcium phosphate to mono and dicalcium phosphate, allowing plants to receive phosphorus minerals. According to Nahas [ 42 ] and Anand et al [ 43 ], organic acids generated by bacteria dissolve insoluble phosphate with a decrease in pH, chelation of cations, and interaction with phosphate on sorption sites in the soil.…”

Single‐cell Raman and functional gene analyses reveal microbial P solubilization in agriculture waste‐modified soils

“…The LEfSe analysis revealed some RM‐sensitive bacteria in three soils, including Bacillus , Actinomadura , and Myxococcia , which have been reported as keystone bacteria in agriculture ecosystems linked to the P solubilization function 41 , 42 , 43 . A further correlation analysis of the Raman‐based phenotypes with the taxonomy‐based genotypes revealed that the abundance of Myxococcota was significantly positively correlated with the proportion of active PSMs.…”

Potential role of rhizosphere microbiome on root exudations and phosphorus uptake of rice under flooded soil culture

“…Dysbiosis, in which the balance of a microbial community is disrupted, has been reported to have deleterious physiological effects on plants (Rosenberg and Zilber-Rosenberg, 2016; Chen et al, 2020; Song et al, 2021). Additionally, members of the phyla Actinobacteria and Firmicutes were recognized as plant growth promoters, notably related to phosphate provision to the plant (Mitra et al, 2022;Mažylytė et al, 2022). Dysbiosis due to the speci c decline in these two phyla in other plant rhizospheres, such as tomato (Solanum lycopersicum), has also been reported to compromise plant health (Lee et al, 2021).…”