Phosphorylation of ATP-Citrate Lyase by Nucleoside Diphosphate Kinase

Abstract

Rat liver nucleoside diphosphate kinase (NDPK) and PC12 cell cytosol were used to determine whether NDPK could function as a protein kinase. NDPK was phosphorylated on its catalytic histidine using [␥-

Nm23/PuF Does Not Directly Stimulate Transcription through the CT Element in Vivo

“…Our data do not eliminate the possibility that Nm23-H2/PuF might indirectly participate in transcription. Other known biochemical activities for the Nm23 protein family include a nonspecific nucleoside diphosphate kinase (Ndpk) activity (6 -8), protein histidine kinase activity (13), and under reducing conditions, protein serine phosphotransferase activity (38), any of which might be linked obliquely to gene regulation, but directly to other processes. We have recently correlated Nm23-H1 structure and function to suppression of tumor motility.…”

“…Considerable investigation has focused on the biochemical mechanism of nm23 in mediating these diverse phenotypes. Although only 17 kDa in size, a number of biochemical functions have been ascribed to Nm23, including a nucleoside diphosphate kinase (NDPK) 1 activity (6 -8), serine phosphorylation (9 -12), a histidine protein kinase activity (13), and a transcriptional stimulatory activity (14 -17). Interactions of Nm23 with small or heterotrimeric G proteins, GAP proteins, or microtubules have been postulated, but have been subject to considerable debate (reviewed in Ref.…”

Advances in development of new tools for the study of phosphohistidine

“…Two isoforms of nucleoside diphosphate kinase (NDPK): Nme1/NDPK-A [9][10][11][12][13] and Nme2/NDPK-B [14][15][16][17][18][19] have been characterized as mammalian His kinases. Other mammalian His kinases exist.…”

Progress on Nme (NDP kinase/Nm23/Awd) gene family-related functions derived from animal model systems: studies on development, cardiovascular disease, and cancer metastasis exemplified

“…Compared to Nme2, there are even fewer known substrates of the kinase activity of Nme1. ATP citrate lyase, the first confirmed substrate of Nme1 acting as protein histidine kinase (Wagner and Vu 1995), is also a substrate to PHP. Its phosphorylation correlates to higher enzymatic activity and to the viability of neuronal cells in culture (Klumpp et al 2009).…”