Photocatalytic electrosensor for label-free and ultrasensitive detection of BRCA1 gene

Qin, Xiaojiao; Xu, Shoufang; Deng, Li; Huang, Rong; Zhang, Xinfeng

doi:10.1016/j.bios.2016.05.076

Cited by 30 publications

(10 citation statements)

References 45 publications

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“…BRCA1, the cancer susceptibility gene containing in human chromosome 17q12-21 region, is responsible for approximately 80 % of breast cancer families [1][2][3][4][5], also ovarian, pancreatic and colon cancers [6,7]. The BRCA1 encoded protein can repair damaged DNA for maintaining the genomic stability, and also control the cell cycle.…”

Section: Introductionmentioning

confidence: 99%

Employing Label‐free Electrochemical Biosensor Based on 3D‐Reduced Graphene Oxide and Polyaniline Nanofibers for Ultrasensitive Detection of Breast Cancer BRCA1 Biomarker

Xia

Chen

et al. 2020

Electroanalysis

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A label‐free DNA biosensor based on three‐dimensional reduced graphene oxide (3D‐rGO) and polyaniline (PANI) nanofibers modified glassy carbon electrode (GCE) was successfully developed for supersensitive detection of breast cancer BRCA1. The results demonstrated that 3D‐rGO and PANI nanofibers had synergic effects for reducing the charge transfer resistance (Rct), meaning a huge enhancement in electrochemical activity of 3D‐rGO‐PANI/GCE. Probe DNA could be immobilized on 3D‐rGO‐PANI/GCE for special and sensitive recognition of target DNA (1.0×10−15–1.0×10−7 M) with a theoretical LOD of 3.01×10−16 M (3S/m). Furthermore, this proposed nano‐biosensor could directly detect BRCA1 in real blood samples.

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Section: Introductionmentioning

confidence: 99%

Employing Label‐free Electrochemical Biosensor Based on 3D‐Reduced Graphene Oxide and Polyaniline Nanofibers for Ultrasensitive Detection of Breast Cancer BRCA1 Biomarker

Xia

Chen

et al. 2020

Electroanalysis

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“…Current techniques for DNA detection, e.g., hybridization assay, usually require complicated and costly fluorescent labeling processes which affect the precision of gene detection due to the degradable labels [ 5 – 7 ]. In recent years, marker-free gene detection techniques are garnering more and more attention and beginning to be recognized as the development trend of gene detection [ 8 , 9 ].…”

Section: Introductionmentioning

confidence: 99%

Detection of DNA oligonucleotides with base mutations by terahertz spectroscopy and microstructures

et al. 2018

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DNA oligonucleotides with a 5-base mutation at the 3'-terminus were investigated by terahertz (THz) spectroscopy in a marker-free manner. The four single-stranded oligonucleotides with 17nt have been detected with specificity on a microfluidic chip, and corroborated by spectral measurements with split-ring resonators. The number of hydrogen bonds formed between the oligonucleotide and its surrounding water molecules, deemed a key contribution to the THz absorption of biological solutions, was explored by molecular dynamics simulations to explain the experimental findings. Our work underlies the feasibility of THz spectroscopy combined with microstructures for marker-free detection of DNA, which may form the basis of a prospective diagnostic tool for studying genic mutation.

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“…Genes as a part of oligonucleotides play a critical role in the genetic disorders, predisposition to diseases, and diversity to aim at drugs and therapy . Human genome mutations always lead to human diseases or disorders .…”

Section: Introductionmentioning

confidence: 99%

A sensitive colorimetric DNA biosensor for specific detection of the HBV gene based on silver‐coated glass slide and G‐quadruplex‐hemin DNAzyme

Liu

Deng

et al. 2017

Journal of Medical Virology

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A sensitive colorimetric DNA biosensor for specific detection of single stranded oligonucleotide (ssDNA) is proposed in this paper. The biosensor is based on silver-coated glass (SCGS) and G-quadruplex-hemin DNAzyme. Capture DNA is immobilized on the surface of SCGS by Ag-S bond. Signal DNA can be used to hybridize with the target DNA which is selected from the Hepatitis B virus(HBV) gene as target HBV DNA, and the HRP-mimicking G-quadruplex-hemin DNAzyme can be formed through the function of a guanine-rich fragment from signal DNA to catalyze the oxidation of 2,2-azinobis(3-ethylbenzothiozoline)-6-sulfonicacid (ABTS ) by H O . The reaction will be monitored along the side of absorbance changes at 418 nm and it can be viewed by naked eye with the change of color as well. Upon addition of target Hepatitis B virus(HBV) DNA, signal DNA could bind on the surface of SCGS, and the concentration of G-quadruplex-hemin DNAzyme immobilizing on the surface of SCGS is depended on that of target HBV DNA. Under the optimum conditions, the absorption was proportional to the concentration of target HBV DNA over the range from 0.5 to 100 nM, with a detection limit of 0.2 nM. In addition, the biosensor is target specific and practicability. This assay might open a new avenue for applying in the diagnosis of HBV disease in the future.

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Photocatalytic electrosensor for label-free and ultrasensitive detection of BRCA1 gene

Cited by 30 publications

References 45 publications

Employing Label‐free Electrochemical Biosensor Based on 3D‐Reduced Graphene Oxide and Polyaniline Nanofibers for Ultrasensitive Detection of Breast Cancer BRCA1 Biomarker

Employing Label‐free Electrochemical Biosensor Based on 3D‐Reduced Graphene Oxide and Polyaniline Nanofibers for Ultrasensitive Detection of Breast Cancer BRCA1 Biomarker

Detection of DNA oligonucleotides with base mutations by terahertz spectroscopy and microstructures

A sensitive colorimetric DNA biosensor for specific detection of the HBV gene based on silver‐coated glass slide and G‐quadruplex‐hemin DNAzyme

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