Photodynamic treatment of yeast cells with the dye Toluidine blue: all-or-none loss of plasma membrane barrier properties

Paardekooper, Michel; Broek, Peter J.A. Van den; Bruijne, Adriaan W. De; Elferink, J. G. R.; Dubbelman, T. M. A. R.; Steveninck, J. Van

doi:10.1016/0005-2736(92)90117-5

Cited by 56 publications

(40 citation statements)

References 19 publications

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“…Our observations are very similar to those obtained for the PDI of K. maxianus with toluidine blue (30,31) and the PDI of C. albicans with hematoporphyrin (3,4). In both cases it was concluded that the plasma membrane became permeable during PDI, but the cell inactivation was attributed to more subtle forms of membrane damage, followed by damage to intracellular targets.…”

Section: Discussionsupporting

confidence: 88%

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Mechanistic Study of the Photodynamic Inactivation of Candida albicans by a Cationic Porphyrin

Lambrechts¹,

Aalders²,

Marle

2005

Antimicrob Agents Chemother

157

126

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Section: Discussionsupporting

confidence: 88%

“…They are easy to cultivate and can be used for research that is too complicated to be performed with higher cells. Yeasts such as Saccharomyces cerevisiae and Kluyveromyces marxianus have been used as model organisms to assess the cell damage induced by PDI in eukaryotic cells (14,31,40,44).…”

mentioning

confidence: 99%

Mechanistic Study of the Photodynamic Inactivation of Candida albicans by a Cationic Porphyrin

Lambrechts¹,

Aalders²,

Marle

2005

Antimicrob Agents Chemother

157

126

View full text Add to dashboard Cite

“…Similarly, several outer membrane and plasma membrane proteins undergo an extensive cross-linking in the early stages of phenothiazine photosensitization of Porphyromonas gingivalis [71] and cationic porphyrin photosensitization of E. coli [72]. -A loss of membrane barrier properties resulting in the leakage of intracellular contents, including a collapse of K þ and ionic balance, represents an important step for loss of clonogenicity in photosensitized bacteria and yeasts, such as S. aureus [73] and, respectively, Kluyveromyces marxianus [74]. Membrane damage is also responsible for the rapid impairment of transport functions in bacteria [72] and yeasts [75]; in general, the photoprocess causes a massive reduction in the transport capacity of a wide variety of solutes, thus leading to a shortage of essential substrates for anabolic and catabolic pathways and providing an important contribution to the drop of cell viability.…”

Section: Photodynamic Inactivation Of Microbial Cells: In Vitro Studiesmentioning

confidence: 99%

Photodynamic therapy in the treatment of microbial infections: Basic principles and perspective applications

et al. 2006

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Background and Objectives: Photodynamic therapy (PDT) appears to be endowed with several favorable features for the treatment of infections originated by microbial pathogens, including a broad spectrum of action, the efficient inactivation of antibiotic-resistant strains, the low mutagenic potential, and the lack of selection of photoresistant microbial cells. Therefore, intensive studies are being pursued in order to define the scope and field of application of this approach. Results: Optimal cytocidal activity against a large variety of bacterial, fungal, and protozoan pathogens has been found to be typical of photosensitizers that are positively charged at physiological pH values (e.g., for the presence of quaternarized amino groups or the association with polylysine moieties) and are characterized by a moderate hydrophobicity (n-octanol/water partition coefficient around 10). These photosensitizers in a micromolar concentration can induce a > 4-5 log decrease in the microbial population after incubation times as short as 5-10 minutes and irradiation under mild experimental conditions, such as fluence-rates around 50 mW/cm 2 and irradiation times shorter than 15 minutes. Conclusions: PDT appears to represent an efficacious alternative modality for the treatment of localized microbial infections through the in situ application of the photosensitizer followed by irradiation of the photosensitizer-loaded infected area. Proposed clinical fields of interest of antimicrobial PDT include the treatment of chronic ulcers, infected burns, acne vulgaris, and a variety of oral infections.

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“…For this reason, there has been an increased interest in developing PDT for local infections treatment. Different types of PS have been proposed in laboratory investigations, including porphyrins [14,15], phenothiazine dyes [16,17], chlorins [18,19], and phthalocyanines [20]. However, complete killing of the microorganisms is not frequently achieved and some of the available PS showed limited effectiveness against Candida biofilms and animal models of candidiasis [15,21].…”

Section: Introductionmentioning

confidence: 99%

Susceptibility of clinical isolates of Candida to photodynamic effects of curcumin

et al. 2011

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Background and ObjectiveThe resistance of Candida species to antifungals represents a major challenge for therapeutic and prophylactic strategies. This study evaluated photodynamic therapy (PDT) mediated by Curcumin (CUR) against clinical isolates of C. albicans, C. tropicalis, and C. glabrata, both in planktonic and biofilm forms.Study Design/Materials and MethodsSuspensions of Candida were treated with three CUR concentrations and exposed to four LED fluences. The protocol that showed the best outcomes for inactivation of the planktonic phase was selected to be evaluated against Candida biofilms. In addition, two higher CUR concentrations were tested. The metabolic activity of biofilms was evaluated by means of XTT reduction assay and the biofilm biomass was evaluated using crystal violet (CV) staining assay. Data were analyzed in a mixed model nested ANOVA, Wilcoxon's nonparametric tests, and the Kruskal–Wallis test (α = 5%).ResultsThe use of CUR in association with light was able to promote a significant antifungal effect against the planktonic form of the yeasts. When using 40 µM of CUR, the metabolic activity of C. albicans, C. glabrata, and C. tropicalis biofilms was reduced by 85%, 85%, and 73%, respectively, at 18 J/cm2. CUR‐mediated PDT also decreased the biofilm biomass of all species evaluated. In addition, CV staining showed that C. albicans isolates were strong biofilm‐forming strains, when compared with C. glabrata and C. tropicalis isolates.ConclusionThe results from the present investigation showed that low CUR concentrations can be highly effective for inactivating Candida isolates when associated with light excitation. Lasers Surg. Med. 43:927‐934, 2011. © 2011 Wiley Periodicals, Inc.

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Photodynamic treatment of yeast cells with the dye Toluidine blue: all-or-none loss of plasma membrane barrier properties

Cited by 56 publications

References 19 publications

Mechanistic Study of the Photodynamic Inactivation of Candida albicans by a Cationic Porphyrin

Mechanistic Study of the Photodynamic Inactivation of Candida albicans by a Cationic Porphyrin

Photodynamic therapy in the treatment of microbial infections: Basic principles and perspective applications

Susceptibility of clinical isolates of Candida to photodynamic effects of curcumin

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