Phylogenetic Analyses Support Validity of Genus <i>Eodinium</i> (Ciliophora, Entodiniomorphida, Ophryoscolecidae)

Cedrola, Franciane; Senra, Marcus Vinicius Xavier; D’Agosto, Marta; Dias, Roberto Júnio Pedroso

doi:10.1111/jeu.12355

Cited by 10 publications

(4 citation statements)

References 29 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…At least four replicates were counted per sample and per ciliate species. The protozoan genera and species were identified according to the size and the shape of cells, skeletal plates (if present), macronucleus, and ciliature arrangement (Dogiel, 1927 ; Ogimoto and Imai, 1981 ; Williams and Coleman, 1992 ; Ito and Imai, 1998 ; Ito et al, 2001 ; Cedrola et al, 2017a , b , 2018 ). Different staining procedures were used to stain skeletal plates (iodine solution), nuclei (methyl green-formalin-saline and chrome-alum-carmine), and infraciliature (pyridinated silver carbonate method) (Ogimoto and Imai, 1981 ).…”

Section: Methodsmentioning

confidence: 99%

Rumen Ciliated Protozoa of the Free-Living European Bison (Bison bonasus, Linnaeus)

et al. 2021

View full text Add to dashboard Cite

This study aims to perform population analysis of the rumen ciliated protozoa of the free-living European bison (wisent, Bison bonasus, Linnaeus). The samples of the rumen fluid from the 18 bison subjected to the controlled culls within the free-ranging population in the Bialowieza primeval forest in Poland were collected and examined. The examined ciliates population consisted of the species of the families Isotrichidae and Ophryoscolecidae. There were 12 genera (Isotricha, Dasytricha, Diplodinium, Elytroplastron, Entodinium, Eodinium, Epidinium, Eremoplastron, Eudiplodinium, Metadinium, Ophryoscolex, and Ostracodinium) and 32 morphospecies of the ciliates. We observed the prevalence of a type B protozoan population (56% animals) with the typical Epidinium and Eudiplodinium genera members. Other examined animals possessed the mixed A–B population with Ophryoscolex genus, distinct for type A ciliate population. The average total ciliates count was 2.77 ± 1.03 × 105/ml (mean ± SD). The most abundant genera were Entodinium, 83%, and Dasytricha, 14%. The abundance of other genera was <1% of the total count. Within the 16 Entodinium species determined, the most abundant species was Entodinium nanellum (16.3% of total ciliates count). The average Shannon–Wiener diversity index was 2.1 ± 0.39, evenness was 0.7 ± 0.11, and species richness was 24 ± 3.0 (mean ± SD). Our study is the first report on the population composition and diversity of rumen ciliates of European bison. The composition and counts of ciliate genera and species were similar to the composition and counts of the rumen ciliated protozoa of American bison and many other kinds of free-living and domestic ruminants. Our European bison ciliate population analysis has shown medium ciliate density and high diversity typical for large free-living ruminants with mixed feeding behavior.

show abstract

Section: Methodsmentioning

confidence: 99%

Rumen Ciliated Protozoa of the Free-Living European Bison (Bison bonasus, Linnaeus)

et al. 2021

View full text Add to dashboard Cite

show abstract

“…To make this section more useful to some readers who need to choose a primer set in analyzing rumen protozoa, we evaluated the primers that have been used commonly with respect to their coverage and specificity using in silico evaluation. Briefly, using TestPrime 1.0, we compared the sequences of each primer set against the recent SILVA non-redundant SSU reference dataset (SSU r132), which contains over 400 reference 18S rRNA gene sequences representing 15 genera and 29 species of protozoa, including C. ventriculi (Kittelmann et al, 2015) and Eodinium posterovesiculatum (Cedrola et al, 2017), within subclass Trichostomatia, which covers all rumen ciliates. Coverage and specificity of each primer set were calculated as below (Raymann et al, 2017 Table 1 summarizes the evaluation results of the primer sets.…”

Section: Primer Coverage and Specificity For Protozoal Community Analmentioning

confidence: 99%

Extending Burk Dehority’s Perspectives on the Role of Ciliate Protozoa in the Rumen

Firkins

Park

et al. 2020

Front. Microbiol.

View full text Add to dashboard Cite

Dr. Burk Dehority was an international expert on the classification and monoculture of ruminal ciliated protozoa. We have summarized many of the advancements in knowledge from his work but also in his scientific way of thinking about interactions of ruminal ciliates with the entire rumen microbial community and animal host. As a dedication to his legacy, an electronic library of high-resolution images and video footage catalogs numerous species and techniques involved in taxonomy, isolation, culture, and ecological assessment of ruminal ciliate species and communities. Considerable promise remains to adapt these landmark approaches to harness eukaryotic cell signaling technology with genomics and transcriptomics to assess cellular mechanisms regulating growth and responsiveness to ruminal environmental conditions. These technologies can be adapted to study how protozoa interact (both antagonism and mutualism) within the entire ruminal microbiota. Thus, advancements and limitations in approaches used are highlighted such that future research questions can be posed to study rumen protozoal contribution to ruminant nutrition and productivity.

show abstract

“…Baroin-Tourancheau [26] state that phylogeny trees based on 18S rRNA present fairly congruent data that often correspond to the taxa defined by means of morphological studies. Molecular studies using the 18S-rRNA gene [27,28] indicate that the morphotypes of E. posterovesiculatum are grouped in a clade with high support values and have high genetic similarity (99.9%), which suggests that they constitute the same species. This agrees with the data obtained through the morphological and morphometric characterization performed in the current study.…”

Section: Discussionmentioning

confidence: 99%

Morphological and Morphometric Characterization of Eodinium posterovesiculatum (Ciliophora, Ophryoscolecidae) in Brazilian Cattle

Fregulia

Cedrola

Bordim

et al. 2020

Braz. arch. biol. technol.

Self Cite

View full text Add to dashboard Cite

The morphological variability of Eodinium posterovesiculatum (Ciliophora, Trichostomatia) has been interpreted in different ways: four distinct species or four morphotypes of the same species. The present study aims to perform morphological and morphometric comparative analysis of the four morphotypes found in cattle from the southeastern region of Brazil. Ruminal content samples were obtained from four Holstein x Gir cattle and fixed at 18.5% formalin for morphological analysis. Morphometry was performed based on individuals stained with Lugol's solution [1]. The infraciliary bands were stained using silver carbonate impregnation technique [2]. Morphological and morphometric characterizations, supported by literature, suggest that the four morphotypes of E. posterovesiculatum are actually a single polymorphic species due to small morphometric differences and mostly identical morphological characters, with the format of the caudal processes being the only morphological characteristic that sets them apart.

show abstract

Phylogenetic Analyses Support Validity of Genus Eodinium (Ciliophora, Entodiniomorphida, Ophryoscolecidae)

Cited by 10 publications

References 29 publications

Rumen Ciliated Protozoa of the Free-Living European Bison (Bison bonasus, Linnaeus)

Rumen Ciliated Protozoa of the Free-Living European Bison (Bison bonasus, Linnaeus)

Extending Burk Dehority’s Perspectives on the Role of Ciliate Protozoa in the Rumen

Morphological and Morphometric Characterization of Eodinium posterovesiculatum (Ciliophora, Ophryoscolecidae) in Brazilian Cattle

Contact Info

Product

Resources

About