Phylogeny of the Genus Lentinula Based on Ribosomal DNA Restriction Fragment Length Polymorphism Analysis

Evidence for Two Independent Lineages of Shiitake of the Americas (Lentinula boryana) Based on rDNA and β-Tubulin Gene Sequences

“…The interrelationships of the Asian and Australasian species have been examined by Hibbett et al (1995) and Nicholson et al (1997), and as many as five independent lineages have been resolved within this group (Hibbett et al, 1998). The analyses reported here support the hypothesis that at least two independent and divergent lineages of Lentinula exist in the Americas.…”

“…The MP tree reported by Thon and Royse (1999) shows an alternative rooting in which L. boryana is paraphyletic. Other analyses indicate that Collybia is more closely related to Lentinula and therefore might serve as a more appropriate outgroup (Hibbett and Vilgalys, 1993;Nicholson et al, 1997). However, attempts to amplify and sequence ␤-tubulin genes using the PCR primers that we report in Thon and Royse (1999) were unsuccessful.…”

“…Pegler]. This work has stressed the importance of understanding the taxonomic and phylogenetic relationships between shiitake and its allies (Hibbett and Donoghue, 1996;Nicholson et al, 1997Nicholson et al, , 1995. A thorough understanding of these relationships are necessary to effectively carry out efforts aimed at developing improved commercial lines of shiitake and conserving the biodiversity present in natural populations of this mushroom (Hibbett and Donoghue, 1996).…”

“…An RFLP analysis of various parts of the rDNA repeat (Nicholson et al, 1997(Nicholson et al, , 1995 identified three distinct clusters, corresponding to the three Asian-Australasian species described by Pegler. Using ITS 1 and 2 sequences, Hibbett et al (1995) Fukuda et al (1994) using mtDNA RFLPs also does not support the monophyly of L. lateritia isolates.…”

The biogeographical speciations of shiitake Lentinula edodes and a new species Lentinula platinedodes sp. nov. found in Cat Tien, South Vietnam

“…VÊn ®Ò lµ cÇn chØ ra ®−îc nh÷ng dÉn chøng cho thÊy nhãm ë T©y b¸n cÇu còng cã quan hÖ trùc tiÕp víi nhãm ë §«ng b¸n cÇu, v× trªn thùc tÕ chóng rÊt bÊt t−¬ng hîp [2,9]. NhiÒu dÉn liÖu thùc nghiÖm vÒ gi ¶i tr×nh tù nhiÒu gene (kÓ c ¶ gene tubulin, gene ty thÓ) còng ®−îc ¸p dông chøng tá qu¸ tr×nh ph©n hãa vµ mèi quan hÖ chAEt chÏ nµy [6,10,13,22]. HiÖn vÉn chØ lµ gi ¶ thiÕt r»ng chóng cã tae tiªn chung mµ ch−a cã dÉn liÖu thuyÕt phôc vÒ kh ¶ n¨ng quan hÖ trùc tiÕp.…”