Physico-chemical characterization and pharmacological activities of polysaccharides from Opuntia microdasys var. rufida cladodes

Jouini, Meriem; Abdelhamid, Amal; Chaouch, Mohamed Aymen; Cerf, Didier Le; Bouraoui, Abderrahman; Majdoub, Hatem; Jannet, Hichem Ben

doi:10.1016/j.ijbiomac.2017.10.003

Cited by 25 publications

(19 citation statements)

References 51 publications

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“…Low methyl-esterified pectin from Opuntia microdasys cladodes exerted an anti-inflammatory effect in mice and reduced gastric ulcer in rats at doses of 100 mg per kg body weight. 41 Similarly, low methyl-esterified pectic substances isolated from common pondweed showed anti-endotoxemic activity in mice by decreasing the expression of TNF-α and IL-1β and increasing the levels of anti-inflammatory cytokines such as IL-10. 42 These studies agree with our results, where low methyl-esterified AP showed a higher immunomodulatory capacity than high methyl-esterified CP used as the control.…”

Section: Discussionmentioning

confidence: 99%

Intestinal anti-inflammatory effects of artichoke pectin and modified pectin fractions in the dextran sulfate sodium model of mice colitis. Artificial neural network modelling of inflammatory markers

et al. 2019

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Section: Discussionmentioning

confidence: 99%

Intestinal anti-inflammatory effects of artichoke pectin and modified pectin fractions in the dextran sulfate sodium model of mice colitis. Artificial neural network modelling of inflammatory markers

et al. 2019

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“…After one hour, the thickness of the right ear of each animal was measured at the same location using vernier calipers, with the left ear as a control (control group). The swelling rate and swelling coefficient were calculated as previously reported [ 48 ]. Ear tissue was collected and stained with hematoxylin and eosin (H&E) for pathological observation.…”

Section: Methodsmentioning

confidence: 99%

Purification, Structural Characterization, and Anti-Inflammatory Effects of a Novel Polysaccharide Isolated from Orostachys fimbriata

Fan

Yang

et al. 2021

Molecules

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The present study elucidated the structural characteristics and anti-inflammatory activity of a novel polysaccharide isolated from Orostachys fimbriata, which is a traditional Chinese medicinal plant. O. fimbriata polysaccharide (OFP) was extracted and subsequently purified by chromatography using a DEAE cellulose-52 and Sephadex G-75 column. The molecular weight was determined as 6.2 kDa. HPGPC and monosaccharide composition analysis revealed a homogeneous polysaccharide containing only Glc. Chromatography and spectral analysis showed that the possible chemical structure consisted of →4)-α-Glcp-(1→ and a small quantity of →4,6)-β-Glcp-(1→ in the main chain and →6)-β-Glcp-(1→, α-Glcp-(1→, and β-Glcp-(1→ in the side chain. Morphological analysis using scanning electron microscopy (SEM) and atomic force microscopy (AFM) indicated that OFP had a multi-branched structure, and the sugar chain molecules of polysaccharide appeared aggregated. OFP was found to exhibit anti-inflammatory activity by reducing the secretion of inflammatory factors in RAW264.7 cells and by decreasing the extent of xylene-induced ear swelling in mice.

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“…The absorption band between 1185 and 1045 cm −1 is related to the stretching vibrations of the glycosidic bonds (C-O, ether linkage) and pyranoid rings (C-C) attributed to polysaccharides that are part of pectins and mucilage (Pereira et al, 2016). According to Lefsih et al (2016) and Jouini et al (2018) the pectins from cladodes of Opuntia ficus indica and Opuntia microdasys can be precipitated with ethanol, explaining the presence of pectins in the samples of mucilage. The absorption bands between 1750 and 1735 cm −1 correspond to C = O stretching vibration of methyl esterified groups (Kpodo et al, 2017) and the regions of 1650-1600 cm −1 are related with the carboxyl group (COOH) (Kpodo et al, 2017;Nejatzadeh-Barandozi & Enferadi, 2012) meanwhile, Synytsya, Čopíková, Matějka., and Machovic (2003) reported the stretching vibrations of carboxylate anion (COO − ) at 1633 cm −1 .…”

Section: Ftir Spectroscopymentioning

confidence: 99%

Mucilage from cladodes ofOpuntia spinuliferaSalm-Dyck: chemical, morphological, structural and thermal characterization

Madera‐Santana

Vargas-Rodríguez

Núñez-Colín

et al. 2018

CyTA - Journal of Food

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The mucilage obtained from cladodes of Opuntia spinulifera has been used in industries and traditional applications. In the present work, the chemical, morphological, thermal and structural features of cladode mucilage from Opuntia spinulifera Salm-Dyck were studied. The 2D 1 H-1 H COSY NMR confirmed ten different residues within the repeating saccharide unit, six with α and four with β configurations. The FTIR showed the presence of galactose and pectins. The XRD detected minerals as calcium salts. The SEM exhibited aggregations and irregular morphology of the particles. The TGA registered the highest mass loss between 246-378°C and the DSC showed the transition state at 55.7°C and at 113.2°C was observed the typical endothermic peak, where it corresponds to the organic compound materials. Mucilago de cladodios de Opuntia spinulifera Salm-Dyck: caracterización química, morfológica, estructural y térmica RESUMEN El mucilago obtenido de cladodios de Opuntia spinulifera ha sido utilizado en varias industrias y en aplicaciones tradicionales. En el presente estudio, se estudiaron las características químicas, morfológicas, térmicas y estructurales del mucilago proveniente de cladodios de Opuntia spinulifera Salm-Dyck. La espectroscopía de correlación-resonancia magnética nuclear (EC-RMN) de 1 H-1 H en 2D confirmó la existencia de 10 residuos diferentes al interior del grupo sacárido que se repite, seis con configuraciones α y cuatro con configuraciones β. El espectrofotómetro de transformada de Fourier (FTIR) constató la presencia de galactosa y pectinas. La difracción de rayos X (DRX) detectó la existencia de minerales en forma de sales de calcio. La microscopía electrónica de barrido (MEB) reveló la presencia de agregaciones, así como la morfología irregular de las partículas. El análisis termogravimétrico (ATG) registró la mayor pérdida de masa entre 246-378°C, mientras que la calorimetría diferencial de barrido (DSC) precisó que se produjo un estado de transición a los 55.7°C, observándose el pico endotérmico típico de los materiales orgánicos compuestos a los 113.2°C.

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Physico-chemical characterization and pharmacological activities of polysaccharides from Opuntia microdasys var. rufida cladodes

Cited by 25 publications

References 51 publications

Intestinal anti-inflammatory effects of artichoke pectin and modified pectin fractions in the dextran sulfate sodium model of mice colitis. Artificial neural network modelling of inflammatory markers

Intestinal anti-inflammatory effects of artichoke pectin and modified pectin fractions in the dextran sulfate sodium model of mice colitis. Artificial neural network modelling of inflammatory markers

Purification, Structural Characterization, and Anti-Inflammatory Effects of a Novel Polysaccharide Isolated from Orostachys fimbriata

Mucilage from cladodes ofOpuntia spinuliferaSalm-Dyck: chemical, morphological, structural and thermal characterization

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