Phytochemical and antibacterial screening of leaf extracts from Cassia singueana Del.(Fabaceae)

Jibril, Saidu; Zakari, Abdu; Kendeson, Christiana Anawuese; Abdullahi, Ibrahim; Idris, MM; Sirat, Hasnah Mohd

doi:10.4314/bajopas.v13i1.15

Cited by 8 publications

(5 citation statements)

References 3 publications

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“…This also concurs with a report by Kareru et al [ 27 ], which showed that the aqueous leaf extracts of S. singueana had higher effects on S. aureus and B. subtilis than it had on E. coli . This is also in agreement with Jibril et al [ 64 ] who demonstrated the broad-spectrum antibacterial effects of methanol and ethyl acetate leaf extracts of S. singueana . However, our findings are partly contrary to the reports of Shawa et al [ 20 ], which suggested that aqueous leaf and root extracts of S. singueana were inactive on S. aureus and Pseudomonas aeruginosa .…”

Section: Discussionsupporting

confidence: 93%

GC-MS Analysis, Antibacterial and Antioxidant Potential of Ethyl Acetate Leaf Extract of Senna singueana (Delile) Grown in Kenya

Nyalo

Omwenga

Ngugi

2022

Evidence-Based Complementary and Alternative Medicine

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Bacterial diseases are a leading cause of mortality and morbidity globally. During bacterial diseases, an elevation of host immune response occurs, which involves the production of free radicals in response to the bacterial infection. The overproduction of free radicals in excess of the antioxidants leads to oxidative stress. Conventional antibiotics are linked to side effects such as hypersensitivity reactions in addition to bacterial pathogens developing resistance against them. Artificial antioxidants are said to be carcinogenic. This study sought to confirm folklore use and validate the antibacterial and antioxidant potential of Senna singueana which has been widely used in the Mbeere community. The in vitro antibacterial potentials of the plant extract were investigated on Bacillus subtilis ATCC 21332, Escherichia coli ATCC 25922, Salmonella typhi ATCC 1408, and Staphylococcus aureus ATCC 25923. Ciprofloxacin (100 µg/ml) drug was used as a standard reference, whereas 5% DMSO was used as a negative reference. The antibacterial tests included disc diffusion and minimum inhibitory and bactericidal concentrations. S. singueana ethyl acetate extract showed broad-spectrum potential against tested bacterial microbes producing mean zones of inhibition (MZI) from 07.67 ± 0.33 to 17.67 ± 0.33 mm. The extract demonstrated a greater effect on Gram-positive than Gram-negative bacterial pathogens. Antibacterial properties of ciprofloxacin were significantly greater in comparison to plant extract in all the dilutions ( p < 0.05 ), while 5% DMSO was inactive against all the tested bacteria. MBC values were greater than MIC values. Antioxidant properties of the extract were determined through scavenging effects of DPPH and hydroxyl radicals (•OH) as well as ferric reducing antioxidant potential (FRAP) assay. S. singueana demonstrated effects against all radicals formed. Additionally, the extract exhibited ferric reducing abilities. The extract also contained various phytocompounds with known antibacterial and antioxidant properties. This study recommends the therapeutic use of S. singueana as an antibacterial as well as an antioxidant agent.

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Section: Discussionsupporting

confidence: 93%

GC-MS Analysis, Antibacterial and Antioxidant Potential of Ethyl Acetate Leaf Extract of Senna singueana (Delile) Grown in Kenya

Nyalo

Omwenga

Ngugi

2022

Evidence-Based Complementary and Alternative Medicine

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“…Four tetrahydroanthracene derivatives which showed significant antibacterial and antiplasmodic activity namely singueanol-I and -II, torosachrysone and germichrysone have been previoulsy isolated from the roots of S. singueana (34). Other compounds which have been isolated from S. singueana include stigmasterol, stigmast-4-en-3-one, stigmast-4,22-dien-3-one, 1-heneicosanol, and hexyl heneicosanoate from the n-hexane leaf extract (35). The flavanoid, luteolin has also been isolated from this plant species previously (36).…”

Section: Discussionmentioning

confidence: 99%

Bioassay guided fractionation of Senna singueana and its potential for development of poultry phytogenic feed additives

et al. 2021

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There has been burgeoning interest in plant-based feed additives following restrictions placed on the use of antibiotic feed additives in many countries. Phytogenic feed additives are recommended to have a range of useful properties to support the growth and development of poultry to a similar level as that obtained by supplementing feed with antibiotics. The aim of this study was to evaluate the antibacterial, anti-lipoxygenase and antioxidant activity, and in vitro safety of fractions and isolated compounds from leaves of Senna singueana. Antibacterial activities of the fractions and isolated compounds were determined against a panel of bacteria using a two-fold serial microdilution assay and qualitative bioautography assays. Anti-lipoxygenase activity was evaluated using the ferrous oxidation-xylenol orange (FOX) method. Antioxidant activity was assessed qualitatively and quantitatively using radical scavenging assays. Dichloromethane and ethyl acetate fractions from solvent-solvent partitioning had the best antibacterial activity with MIC values ranging from 156 to 313 µg/ml. Fractions obtained from column chromatography had significant to weak antibacterial activity with MIC values ranging from 50 to 1,250 µg/ml. Bioautography showed clear bands of bacterial inhibition, indicating the presence of a number of active compounds in several fractions. The ethyl acetate fraction and all the tested column fractions had potent anti-lipoxygenase activity with IC 50 values of ≤2.5 µg/ml which were lower than that of quercetin (positive control), indicating anti-inflammatory potential. The ethyl acetate fraction and several column fractions had powerful antioxidant activity with IC 50 values of ≤5 µg/ml in the ABTS assay. Cytotoxicity values against Vero kidney cells ranged from LC 50 = 40.0-989.3 µg/ml. Bioassay-guided fractionation led to the isolation and identification of a known bioactive compound, luteolin. S. singueana is a promising candidate for the development of poultry phytogenic feed additives.

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“…The C. singueana extract exhibited appropriate values for phenolic content (120.01 mg GAE/g dry extract), DPPH (IC50: 9.35 μg/mL), and α-glucosidase inhibition (I%: 90.47%) (17). In a phytochemical screening performed on the methanolic extract of C. angustifolia root and its fractions, the hexane fraction showed qualitative amounts of terpenoids, anthraquinones, and coumarins (18).…”

Section: Discussionmentioning

confidence: 99%

“…C. sieberiana exhibited significant activities for 15-LOX inhibition (80.93% inhibition) and FRAP (2120 μmol Fe2+/g dry extract). The extracts of C. singueana and C. sieberiana contain αglucosidase inhibitors, antioxidants, and antiinflammatory compounds, making them useful for the treatment of ulcers and diabetes (17).…”

Section: Discussionmentioning

confidence: 99%

Cassia angustifolia Vahl. Leaves: Determination of Total Phenolic and Sennoside Contents of Different Fractions in Comparison with Their α- Glucosidase and Tyrosinase Inhibitory Effects

Morid Ahmadi,

Mojtabavi,

Ghadami

et al. 2024

Iran J Pharm Res

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Background: Cassia angustifolia Vahl. (Senna) is a medicinal plant containing anthraquinone compounds such as sennoside. Senna is primarily valued for its laxative properties. In Persian medicine, this plant has been also used to treat various disorders such as diabetes and skin hyperpigmentation. Previous studies have shown that different species of senna, such as C. articulata, C. alata, C. Siamea, C. Surattensis inhibit alpha-amylase and α-glucosidase enzymes. To the best of our knowledge, no previous evidence is available on tyrosinase and α-glucosidase inhibitory effects of the extract and different fractions of C. angustifolia leaves. Objectives: The purpose of this study was to investigate the inhibitory effect of the methanol-water extract and different fractions (hexane, chloroform, ethyl acetate, and remaining crude extract) of senna against tyrosinase and α-glucosidase and to investigate their total phenolic and sennoside B contents. Results: Our findings depicted that the methanol-water extract and fractions had no significant anti-tyrosinase activity; however, some fractions were active toward α-glucosidase. The hexane fraction and the remaining crude extract demonstrated the highest inhibition on α-glucosidase compared to acarbose (positive control). In addition, the ethyl acetate fraction contains high phenolic and hydroxy anthraquinone derivatives based on the amount of sennoside B contents equivalent to 382.25 μg/mL of gallic acid and 1.525% of sennoside B, respectively. Moreover, no correlation was observed between the phenolic and sennoside contents of different fractions and their α-glucosidase inhibitory effect. Conclusions: Considering the α-glucosidase inhibition results, the hexane fraction of C. angustifolia can be a valuable fraction for in vitro and in vivo antidiabetic studies as well as further phytochemical studies. Further studies to identify the active substances and the exact mechanism of the bioactive ingredients on the inhibitory effects of α-glucosidase can provide promising results in the future.

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Phytochemical and antibacterial screening of leaf extracts from Cassia singueana Del.(Fabaceae)

Abstract: No Abstract.

Cited by 8 publications

References 3 publications

GC-MS Analysis, Antibacterial and Antioxidant Potential of Ethyl Acetate Leaf Extract of Senna singueana (Delile) Grown in Kenya

GC-MS Analysis, Antibacterial and Antioxidant Potential of Ethyl Acetate Leaf Extract of Senna singueana (Delile) Grown in Kenya

Bioassay guided fractionation of Senna singueana and its potential for development of poultry phytogenic feed additives

Cassia angustifolia Vahl. Leaves: Determination of Total Phenolic and Sennoside Contents of Different Fractions in Comparison with Their α- Glucosidase and Tyrosinase Inhibitory Effects

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