Phytochemical, antioxidant and antimicrobial properties of Litsea angulata extracts

Kuspradini, Harlinda; Wulandari, Indah; Putri, Agmi Sinta; Tiya, Sabeti Yulis; Kusuma, Irawan Wijaya

doi:10.12688/f1000research.16620.1

Cited by 17 publications

(24 citation statements)

References 8 publications

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“…The IC50 values with regards to different used solvents and plant parts were, in increasing order, as follows: bark, ethyl acetate; leaves-bark-branch, ethanol; branch, ethyl acetate; leaves, ethyl acetate; branch and leaves, n-hexane. Raw absorbance data from which IC50 values were calculated are shown in Dataset 1 15 .…”

Section: Resultsmentioning

confidence: 99%

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Phytochemical, antioxidant and antimicrobial properties of Litsea angulata extracts

Kuspradini

Wulandari²,

Putri

et al. 2019

F1000Res

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Background: Litsea angulata is a plant species belonging to Lauraceae family that is distributed throughout Indonesia, Malaysia, and New Guinea. The seeds have been traditionally used by local people in Kalimantan, Indonesia for the treatment of boils; however, there is no information about the potency of its branch, bark and leaves yet. This study aimed to determine the antioxidant, antimicrobial activity as well as the phytochemical constituent of Litsea angulata branch, bark, and leaves. Methods: Extraction was performed by successive maceration method using n-hexane, ethyl acetate, and ethanol solvent. Antioxidant activity was evaluated by DPPH radical scavenging assay. The antimicrobial activity using the 96 well-plate microdilution broth method against Staphylococcus aureus and Streptococcus mutans. Results: Based on the phytochemical analysis, it showed that extract of L. angulata contains alkaloids, flavonoids, tannins, terpenoids, and coumarin. The results showed that all extracts of plant samples displayed the ability to inhibit DPPH free radical formation and all tested microorganisms. Conclusions: L. angulata contains secondary metabolites such as alkaloids, flavonoids, tannins, terpenoids, carotenoids, and coumarin. The antioxidant activity on different plant extracts was a range as very strong to weak capacity. All extracts in this study could inhibit the growth of S. aureus and S. mutans.

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Section: Resultsmentioning

confidence: 99%

“…Data include the absorbance values obtained from the DPPH scavenging assay, and the resultant IC50 values generated. DOI: https://doi.org/10.5256/f1000research.16620.d223677 15 .…”

Section: Data Availabilitymentioning

confidence: 99%

Phytochemical, antioxidant and antimicrobial properties of Litsea angulata extracts

Kuspradini

Wulandari²,

Putri

et al. 2019

F1000Res

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“…The presence of bioactive metabolites in the extracts such as flavonoids, tannins, saponins, glycosides, alkaloids, phenolics, steroids and anthraquinone as reported earlier is likely to be responsible for the good antimicrobial property. Polyphenols and other bioactive molecules regulate the synthesis of nucleic acids of both Gram-negative and Gram-positive bacteria; that is why the proper zone of inhibition has been observed in the assay 13 , 56 , 70 . Perumal showed that plants with high caffeic acid levels had shown significant antimicrobial activity.…”

Section: Discussionmentioning

confidence: 99%

Phytochemical composition analysis and evaluation of in vitro medicinal properties and cytotoxicity of five wild weeds: A comparative study

et al. 2020

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Background: Medicinal plants are a source of phytochemicals and they are used for the treatment of several oxidative stress-related or other diseases for their effectiveness, low toxicity and easy availability. Five traditionally used and less characterized herbaceous weeds of West Bengal, India, namely, Heliotropium indicum, Tridax procumbens, Cleome rutidosperma, Commelina benghalensis and Euphorbia hirta, were investigated for the current research study. Methods: Aqueous and 70% ethanolic extracts of the leaves were analyzed for estimation of essential phytochemicals and to evaluate their in vitro antioxidant status, medicinal properties and cytotoxic effects. To the best of our knowledge, several assays and comparative evaluations using these herbs are reported for the first time. For quantitative study, UV-vis spectrophotometry and high-performance liquid chromatography with diode array detector HPLC-DAD techniques were used. Antibacterial properties were investigated using the Kirby-Bauer disc diffusion method. For in vitro anti-lithiatic study, a titration method was used. The cell viability assay was done using peripheral blood mononuclear cells. Results: The aqueous extract exhibits higher content of polyphenols, flavonoids, tannins and inhibition percentage values for free radical scavenging assays, whereas the 70% ethanolic extract exhibits higher content of alkaloids and cardiac glycosides. HPLC-DAD analysis of 70% ethanolic extracts led us to identify 10 predominant phenolic constituents. Euphorbia hirta extracts showed minimum cytotoxicity (cell death ~2.5% and 4% in water and 70% ethanolic extract, respectively), whereas Cleome rutidosperma and Tridax procumbens’ 70% ethanolic extracts showed higher cell death (~13% and 28%, respectively), compared with the control (cell death ~10-12%). Conclusions: The study concluded that of all the medicinal weeds selected for the current study, Euphorbia hirta possesses the highest amount of bioactive compounds and hence exhibits the highest in vitro antioxidant activity and promising in vitro medicinal properties.

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“…Plant extracts have been reported to have numerous protective roles due to their phytochemical contents, which contributes to a large extent towards their antioxidant and antimicrobial activities 22 . Oxidative stress, which leads to the production of free radicals in the body, plays a major role in the development of chronic and degenerative ailments such as cancer, arthritis, aging, autoimmune disorders, cardiovascular and neurodegenerative diseases 23 .…”

Section: Discussionmentioning

confidence: 99%

Assessment of the phytochemical, antioxidant and antibacterial activities of Heteromorpha arborescens (Spreng.) Cham & Schltdl. leaf extracts

2020

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Background: Heteromorpha arborescens (Spreng.) Cham. and Schltdl (Apiaceae) is widely used traditionally for the treatment of a wide range of diseases in Southern and Eastern Africa. Although previous studies have reported the biological activities of hexane, ethyl acetate and methanol extracts of H. arborescens leaves, there is no scientific information on the phytochemical contents, antioxidant and antibacterial activities of acetone, ethanol, aqueous and blanched extracts. This study is therefore aimed to investigate and compare the phytochemical contents, antioxidant and antibacterial activities of acetone, ethanol, aqueous and blanched extracts of H. arborescens leaves. Methods: Phytochemical analysis for the total phenolic, flavonoid, proanthocyanidin, alkaloid and saponin contents of all the fractions were determined by spectroscopic methods, while the free radical scavenging potential of the extracts were evaluated using DPPH, ABTS radical scavenging and total antioxidant capacity assays. Micro dilution method was used to determine the Minimum Inhibitory Concentrations (MIC) of H. arborescens leaf extracts against Bacillus pumilus, Staphylococcus epidermidis, Staphylococcus aureus, Escherichia coli and Klebsiella pneumoniae. Results: Total phenol content of the extracts ranged between 15.10 mg GAE/g- 42.50 mg GAE/g, proanthocyanidin was 459-8402.1 mg QE/g, and flavonoid content of 109.24-235.79 mg QE/g. In addition, alkaloids (5.59%) and saponins (23.33%) were present in significant amounts. Based on the IC50 values, the ethanol extract exhibited the highest total antioxidant activity (0.013 mg/mL) with highest inhibition against DPPH and ABTS radicals (0.06 and 0.049 mg/mL respectively). Considerable antibacterial activities were observed in the acetone, ethanol and blanched extracts with MIC values ranging from 1.563-12.5 mg/mL; however, the aqueous extract was inactive against all the bacteria strains. Conclusion: The study suggests that H. arborescens leaves could be a valuable source of bioactive compounds. Although the blanching process significantly decreased polyphenolic contents and antioxidant activities of the extracts, it increased the antibacterial compounds.

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Phytochemical, antioxidant and antimicrobial properties of Litsea angulata extracts

Cited by 17 publications

References 8 publications

Phytochemical, antioxidant and antimicrobial properties of Litsea angulata extracts

Phytochemical, antioxidant and antimicrobial properties of Litsea angulata extracts

Phytochemical composition analysis and evaluation of in vitro medicinal properties and cytotoxicity of five wild weeds: A comparative study

Assessment of the phytochemical, antioxidant and antibacterial activities of Heteromorpha arborescens (Spreng.) Cham & Schltdl. leaf extracts

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