Phytochemical screening and enzymatic and antioxidant activities of Erythrina suberosa (Roxb) bark

Abstract

A BSTRACT Background: This study aimed to evaluate the phytochemicals screening of Erythrina suberosa (Roxb) bark and to analyze the enzymatic activities of its various organic fractions. Materials and Methods: Crude methanolic fraction of E. suberosa (Roxb) bark and its respective fractions were screened for the presence of different phytochemicals with different reagents. On the basis of increasing order of polarity, different organic solvents were used to obtain different fractions. Enzymatic studies were performed on crude methanolic extract of the plant. All the assays were performed under standard in vitro conditions. Results: The phytochemical analysis shows the presence of alkaloids, phenols, triterpenoids, phytosterols, and flavonoids. Phenolic compounds and flavonoids are the major constituents of the plant. In anticholinesterase assay, the percent inhibition of standard drug (eserine) was 91.27 ± 1.17 and the half maximal inhibitory concentration (IC 50 ) was 0.04 ± 0.0001. For α-glucosidase inhibition, the IC 50 value for Dichloromethane fraction was 8.45 ± 0.13, for Methanol fraction it was 64.24 ± 0.15, and for aqueous fraction it was 42.62 ± 0.17 as compared with standard IC 50 that is 37.42 (acarbose). Furthermore, results show that all fractions have potential against anti-urease enzyme, but DCM fraction of crude aqueous extract has significant IC 50 value (45.26 ± 0.13) than other fractions. Conclusion: Keeping in view all the results, it is evident that the plant can be used in future for formulating effective drugs against many ailments. Secondary metabolites and their derivatives possess different biological activities, for example, .g. flavonoids in cancer, asthma, and Alzheimer. Furthermore, the extracts of this plant can be used in their crude form, which is an addition to the complementary and alternative treatment strategies.

Exploring the potential of a Ephedra alata leaf extract: Phytochemical analysis, antioxidant activity, antibacterial properties, and green synthesis of ZnO nanoparticles for photocatalytic degradation of methylene blue

“…The appearance of a violet ring under the brown ring in the acetic acid layer is a distinct indicator of the presence of cardenolide glycosides. The test also allows monitoring additional color changes, such as the appearance of a violet ring beneath the brown ring in the acetic acid layer ( Ahmed et al, 2020 ).…”

Proximate Composition and Sensory Evaluation Between Artificially Ripened and Naturally Ripened Pineapples (<em>Ananas comosus</em>)

“…Reducing sugar was determined by Fehling's solution as described previously [19]. A 5 ml pineapple juice was taken in a 100 ml volumetric flask and filled the volume with lab water and mixed efficiently.…”

Proximate Composition and Sensory Evaluation Between Artificially Ripened and Naturally Ripened Pineapples (Ananas comosus)

“…Reducing sugar was determined by Fehling's solution as described previously (Aziz et al, 2020). A 5 mL pineapple juice was taken in a 100 mL volumetric flask and filled volume with lab water and mixed efficiently.…”