Pigs expressing salivary phytase produce low-phosphorus manure

Golovan, Serguei P.; Meidinger, Roy G.; Ajakaiye, A.; Cottrill, Michael; Wiederkehr, Miles Z.; Barney, D.J.; Plante, C.; Pollard, J.W.; Fan, Ming Z.; Hayes, Michael; Laursen, Jesper; Hjorth, J.; Hacker, R. R.; Phillips, J. P.; Forsberg, Cecil W.

doi:10.1038/90788

Cited by 333 publications

(170 citation statements)

References 34 publications

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“…Without the bacterial enzyme, the phytate phosphorus passes undigested into manure and pollutes the environment. With the bacterial enzyme, the fecal phosphorus output was reduced up to 75% [86]. These environmentally friendly pigs are expected to enter the commercial production chains within the next few years.…”

Section: Dietary Modifications Of Animal Productsmentioning

confidence: 99%

The contribution of farm animals to human health

Kues¹

2004

Trends in Biotechnology

117

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Section: Dietary Modifications Of Animal Productsmentioning

confidence: 99%

The contribution of farm animals to human health

Kues¹

2004

Trends in Biotechnology

117

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“…The accumulation of monoester Po in soil occurs because the most animals are unable to digest phytic acid (inositol hexaphosphate), which accumulates in manures and, consequently, in soils treated with this waste. In animal feed, this kind of organic P dramatically reduces the efficiency of P absorption by animals and creates an environmental problem due the P-richness of the manure (Golovan et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Spectroscopic quantification of soil phosphorus forms by 31p-nmr after nine years of organic or mineral fertilization

Gatiboni

Brunetto

Rheinheimer

et al. 2013

Rev. Bras. Ciênc. Solo

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SUMMARYLong-standing applications of mineral fertilizers or types of organic wastes such as manure can cause phosphorus (P) accumulation and changes in the accumulated P forms in the soil. The objective of this research was to evaluate the forms of P accumulated in soils treated with mineral fertilizer or different types of manure in a long-term experiment. Soil was sampled from the 0-5 cm layer of plots fertilized with five different nutrient sources for nine years: 1) control without fertilizer; 2) mineral fertilizer at recommended rates for local conditions; 3) 5 t ha -1 year -1 of moist poultry litter; 4) 60 m 3 ha -1 year -1 of liquid cattle manure and 5) 40 m 3 ha -1 year -1 of liquid swine manure. The 31 P-NMR spectra of soil extracts detected the following P compounds: orthophosphate, pyrophosphate, inositol phosphate, glycerophosphate, and DNA. The use of organic or mineral fertilizer over nine years did not change the soil P forms but influenced their concentration. Fertilization with mineral or organic fertilizers stimulated P accumulation in inorganic forms. Highest inositol phosphate levels were observed after fertilization with any kind of manure and highest organic P concentration in glycerophosphate form in after mineral or no fertilization.Index terms: glycerophosphate, manure, phytate, organic phosphorus, organic fertilization.(1) Received for publication on October 26, 2011 and approved on January 22, 2013.

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“…Recently, one group (Golovan et al, 2001) took a novel approach to improving dietary InsP 6 digestion. They created transgenic pigs that constitutively secrete microbial phytase from their salivary glands (Golovan et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

Avian multiple inositol polyphosphate phosphatase is an active phytase that can be engineered to help ameliorate the planet's “phosphate crisis”

Cho

Choi

Darden

et al. 2006

Journal of Biotechnology

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Contemporary phytase research is primarily concerned with ameliorating the problem of inadequate digestion of inositol hexakisphosphate (phytate; InsP 6 ) in monogastric farm animal feed, so as to reduce the pollution that results from the high phosphate content of the manure. In the current study we pursue a new, safe and cost-effective solution. We demonstrate that the rate of hydrolysis of InsP 6 by recombinant avian MINPP (0.7 μmol/mg protein/min) defines it as by far the most active phytase found to date in any animal cell (the corresponding activity of recombinant mammalian MINPP is only 0.006 μmol/mg protein/min). Although avian MINPP has less than 20% sequence identity with microbial phytases, we create a homology model of MINPP in which it is predicted that the structure of the phytase active site is well-conserved. This model is validated by site-directed mutagenesis and by use of a substrate analogue, scyllo-InsP 6 , which we demonstrate is only a weak MINPP substrate. In a model chicken cell line, we overexpressed a mutant form of MINPP that is secretion-competent. This version of the enzyme was actively secreted without affecting either cell viability or the cellular levels of any inositol phosphates. Our studies offer a genetic strategy for greatly improving dietary InsP 6 digestion in poultry.

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Pigs expressing salivary phytase produce low-phosphorus manure

Cited by 333 publications

References 34 publications

The contribution of farm animals to human health

The contribution of farm animals to human health

Spectroscopic quantification of soil phosphorus forms by 31p-nmr after nine years of organic or mineral fertilization

Avian multiple inositol polyphosphate phosphatase is an active phytase that can be engineered to help ameliorate the planet's “phosphate crisis”

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