Pivotal role of sugar fluxes between the inner integument and endosperm in lipid synthesis during seed ontogeny in Jatropha curcas L.

Chaitanya, Bharatula Sri Krishna; Kumar, S.; Anjaneyulu, Enti; Sastry, P. S. N.; Reddy, A. Ramachandra

doi:10.1016/j.indcrop.2015.08.035

Cited by 7 publications

(5 citation statements)

References 22 publications

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“…In developing kernels, the biological processes of oil biosynthesis are complex and involve carbohydrate decomposition, pyruvate, and acetyl-CoA formation, FA carbon chain extension, and TAG formation ( Rawsthorne, 2002 ; Alonso et al, 2011 ; Lin et al, 2017 ). Meanwhile, oil accumulation is affected by starch synthesis, energy metabolism, amino acid metabolism ( Ekman et al, 2008 ; Andriotis et al, 2012 ; Chaitanya et al, 2015 ). In our study, we identified 56 proteins related to 46 enzymes leading to oil accumulation and 10 of the proteins showed significant downregulation in the FSK group.…”

Section: Discussionmentioning

confidence: 99%

Effects of Fruit Shading on Gene and Protein Expression During Starch and Oil Accumulation in Developing Styrax tonkinensis Kernels

Chen

Zhang

et al. 2022

Front. Plant Sci.

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Styrax tonkinensis has great potential as a biofuel feedstock source having industrial oilseeds with excellent fatty acids (FAs) composition and good fuel properties. Photosynthesis in the developing pericarp could affect the carbon distribution in kernel. During kernel development, more carbon sources are allocated to starch rather than lipid, when the pericarp photosynthesis is reduced by fruit shading treatment. After shading the fruits at 50 days after flowering (DAF), samples of shaded fruit (FSK) and controls (CK) were collected at 80 DAF and analyzed using the proteomic method. We identified 3,181 proteins, of which 277 were differentially expressed proteins, all downregulated in the FSK group. There were 56 proteins found involved in carbohydrate metabolism and lipid biosynthesis leading to oil accumulation with their iTRAQ ratios of FSK/CK ranging from 0.7123 to 1.1075. According to the qRT-PCR analyses, the key genes related to FA and triacylglycerol (TAG) biosynthesis were significantly downregulated between 60 and 90 DAF especially at 80 DAF, while the key genes involved in starch biosynthesis and FA desaturase had no significant difference between the two groups at 80 DAF. Fruit shading is a negative treatment for lipid accumulation but not starch accumulation by restraining enzymic protein expression involved in FA and TAG biosynthesis during S. tonkinensis kernel development.

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Section: Discussionmentioning

confidence: 99%

Effects of Fruit Shading on Gene and Protein Expression During Starch and Oil Accumulation in Developing Styrax tonkinensis Kernels

Chen

Zhang

et al. 2022

Front. Plant Sci.

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“…In J. curcas, the inner integument has the ability to take up both sucrose and glucose, a rapid uptake of glucose by the developing endosperm fueled glycerolipid synthesis. Thus, the inner integument acts as a transient storage tissue and later the developing endosperm was responsible for synthesis and accumulation of lipids in Jatropha seed [26].…”

Section: Discussionmentioning

confidence: 99%

Potential Technological Use of Reserves of <i>Jatropha curcas</i> and <i>J. macrocarpa</i> Griseb. Seeds

Tavecchio¹,

Dardanelli²,

Reguera³

et al. 2019

AJPS

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J. curcas and J. macrocarpa are useful for restoring degraded areas and their seeds contain oils for biodiesel production. The aim of the work was to determine the reserve substances in the endosperm and the embryo of J. curcas and J. macrocarpa which is important in understanding the germination process, the establishment of these species and its industrial employment. Seeds were imbibed in distilled water for 24 h, to facilitate removal of seed coat with the aim to separate the embryo and nutritive tissues. In both species, the endosperm contained aleurone grains consisting of a crystalloid and globoid, lipids of red color and the starch was not observed. Four major fatty acids were determined in J. curcas seed: oleic, palmitic, stearic, palmitoleic and oleic fatty acid represents about 70% oil content. Oleic acid was the most abundant in J. macrocarpa seeds, while, there was not palmitoleic acid. Seed with predominantly unsaturated fatty acids is ideal for biodiesel industry. The means of the sugar content were: 14.3 µg/mg in endosperm and 104.76 µg/mg in embryo of J. curcas and 6.48 µg/mg in endosperm and 59.20 µg/mg in embryo of J. macrocarpa. The means of the protein content were: 4.2 µg/mg in endosperm and 45.02 µg/mg in embryo of J. curcas and 3.26 µg/mg in endosperm and 31.08 µg/mg in embryo of J. macrocarpa. Sugar and protein contents of Jatropha seeds were significantly higher in embryo in both species

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“…9 In addition, the proportional distribution of seed storage products into lipid, protein, starch and sugar depends on the balance between metabolic pathways during kernel development and varies with species. [14][15][16] Wu et al 17 analyzed the oil accumulation pattern in developing S. tonkinensis kernels by examining differentially expressed unigenes (DEGs). They drafted a map of FA and triacylglycerol (TAG) biosynthesis in plastids and the endoplasmic reticulum (ER) based on related unigenes identified in their transcriptome analysis.…”

Section: Introductionmentioning

confidence: 99%

Proteomic analysis of metabolic mechanisms associated with fatty acid biosynthesis during Styrax tonkinensis kernel development

Wang

et al. 2021

J Sci Food Agric

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BACKGROUND: Styrax tonkinensis is a white-flowered tree with considerable potential as a feedstock source for biodiesel production from the oily seed contained within its nutlike drupes. Transcriptome changes during oil accumulation have been previously reported, but not concurrent changes in the proteome. RESULTS: Using proteomic analysis of samples collected at 50, 70, 100 and 130 days after flowering (DAF), we identified 1472 differentially expressed proteins (DEPs). Based on their expression patterns, we grouped the DEPs into nine clusters and analyzed the pathway enrichment. Proteins related to starch and sucrose metabolism were most abundant at 50 DAF. Proteins involved in fatty acid (FA) biosynthesis were mainly grouped into a cluster that peaked at 70 DAF. Proteins related to protein processing in endoplasmic reticulum had two major patterns, trending either upwards or downwards, while proteins involved in amino acid biosynthesis showed more complex relationships. We identified 42 key enzymes involved in lipid accumulation during kernel development, including the acetyl-CoA carboxylase complex (ACC) and the pyruvate dehydrogenase complex (PDC). One oil body membrane protein, oleosin, continuously increased during kernel development.CONCLUSION: A regulatory network of oil accumulation processes was built based on protein and available transcriptome expression data, which were in good temporal agreement. This analysis placed ACC and PDC in the center of the network, suggesting that the glycolytic provision of substrate plays a central regulatory role in FA biosynthesis and oil accumulation.

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Pivotal role of sugar fluxes between the inner integument and endosperm in lipid synthesis during seed ontogeny in Jatropha curcas L.

Cited by 7 publications

References 22 publications

Effects of Fruit Shading on Gene and Protein Expression During Starch and Oil Accumulation in Developing Styrax tonkinensis Kernels

Effects of Fruit Shading on Gene and Protein Expression During Starch and Oil Accumulation in Developing Styrax tonkinensis Kernels

Potential Technological Use of Reserves of <i>Jatropha curcas</i> and <i>J. macrocarpa</i> Griseb. Seeds

Proteomic analysis of metabolic mechanisms associated with fatty acid biosynthesis during Styrax tonkinensis kernel development

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Pivotal role of sugar fluxes between the inner integument and endosperm in lipid synthesis during seed ontogeny in Jatropha curcas L.

Cited by 7 publications

References 22 publications

Effects of Fruit Shading on Gene and Protein Expression During Starch and Oil Accumulation in Developing Styrax tonkinensis Kernels

Effects of Fruit Shading on Gene and Protein Expression During Starch and Oil Accumulation in Developing Styrax tonkinensis Kernels

Potential Technological Use of Reserves of &lt;i&gt;Jatropha curcas&lt;/i&gt; and &lt;i&gt;J. macrocarpa&lt;/i&gt; Griseb. Seeds

Proteomic analysis of metabolic mechanisms associated with fatty acid biosynthesis during Styrax tonkinensis kernel development

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Potential Technological Use of Reserves of <i>Jatropha curcas</i> and <i>J. macrocarpa</i> Griseb. Seeds