Plant Regeneration Through Indirect Somatic Embryogenesis in Coelogyne Cristata Orchid

Naing, Aung Htay; Chung, Jae Dong; Lim, Ki Byung

doi:10.4236/ajps.2011.22028

Cited by 24 publications

(14 citation statements)

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“…Therefore, it would be interesting to study the influence of sucrose combined with an osmotic agent such as mannitol and/or of a sequential supply of high concentration. Recently, Naing et al (2011) reported that the capacity of somatic embryogenesis observed on MS medium was extremely low as compared to that on 1/2 MS medium. However, to our best knowledge, there was no information about effect of basal medium on secondary embryogenesis so far.…”

Section: Secondary Somatic Embryogenesismentioning

confidence: 99%

Primary and secondary somatic embryogenesis in Chrysanthemum cv. Euro

Naing

Kim

Yun

et al. 2012

Plant Cell Tiss Organ Cult

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Somatic embryogenesis was induced from in vivo grown leaf explants of Chrysanthemum cv. Euro incubated on Murashige and Skoog (MS) medium supplemented with 2.0 mg/L 2,4-dichlorophenoxyacetic acid and 2.0 mg/L Kinetin, yielding the highest mean number of embryos (42 ± 5.97) per explant after 5 weeks of culture. We evaluated the effects of basal medium, various concentrations of sucrose, and timentin on the proliferation of secondary somatic embryos. MS medium was observed to be the more effective in promoting the proliferation of somatic embryos than half-strength Murashige and Skoog (1/2MS). In addition, timentin was also more efficient in induction of secondary embryogenesis than sucrose. Whole plantlets were obtained by culturing of secondary embryos on hormone-free MS medium and successfully acclimated in the green house.

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Section: Secondary Somatic Embryogenesismentioning

confidence: 99%

Primary and secondary somatic embryogenesis in Chrysanthemum cv. Euro

Naing

Kim

Yun

et al. 2012

Plant Cell Tiss Organ Cult

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“…In vitro culture has proven as a better method for plant propagation that gives a great hope in plant cultivation especially orchid because this method can be used for orchid propagation in large quantities in a relative short of time (Naing et al, 2011). The succeed of in vitro culture depends on many factors, such as the explants selection, medium type, growth regulator, and nutrient availability.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Callus Induction from Leaf Explants of Vanda sp Stimulated by 2,4-D

2017

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The addition of growth regulator is one of the critical success factors in in vitro cultures. 2,4-D as a plant regulator in media can stimulate the cell division, enlargement of the explants and promotes the formation and growth of callus. The purpose of this study was to determine the time of callus formation and to determine the best concentration of 2.4-D in inducing the growth of callus from leaf explants of Vanda sp. This research was conducted by experiment with completely randomized design, which consists of six levels of treatment concentration of 2,4-D i.e. 0 ppm; 1 ppm; 1.5 ppm; 2 ppm; 2.5 ppm; and 3 ppm. The parameters observed were the percentage of callus formation and the form of callus from Vanda sp leaf explants. The results were statistically analyzed by using MINITAB program version 17. Analysis of variance (ANOVA) was performed and the difference between means score/ value was separated by F test at p < 0.05. The results showed that 2,4-D treatment give significant effect (F 5,12 = 3,20; p = 0,046 < 0,05) on the callus growth time and its percentage. Application of 2 ppm 2.4-D was the best concentration for accelerating the callus growth time (14.3 days after planting) and increasing the percentage of callus formation (83.3%). Most of callus type were proliferative callus (36.11%) and senescence callus (11.11%). The results of this research are very important to grow the callus from Vanda leaves orchid explant because it is very diffucult to grow.

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“…[11], and Coelogyne cristata (Lindl.) [12] occurred on the medium that contained both auxin and cytokinin, where the auxin concentration was higher than the cytokinin. The optimum concentration of NAA in this experiment is 0.05 mg ⋅ L −1 , supposing that it is lower than the previous study on somatic embryogenesis in Vanda [5,13].…”

Section: Initiation and Proliferation Of Embryogenic Callusmentioning

confidence: 99%