Plasma and urine metabonomics coupled with UPLC-Q-TOF/MS and multivariate data analysis on potential biomarkers in anemia and hematinic effects of steamed notoginseng

Zhang, Yin; Shi, Jianfeng; Fei, Qianqian; Huang, Yiping; Zhu, Fang; Wang, Jing; Tang, Daoquan; Chen, Bin

doi:10.1016/j.phytol.2020.05.003

Cited by 3 publications

(3 citation statements)

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“…The detection technologies of metabonomics mainly include gas chromatography–mass spectrometry (GC–MS), ultra‐performance liquid chromatography/quadrupole time‐of‐flight mass spectrometry/mass spectrometry (UPLC–Q/TOF–MS/MS) and nuclear magnetic resonance. Among these detection technologies, UPLC–Q/TOF–MS/MS has the advantages of high sensitivity and high resolution (Wan et al, 2020 ; Zhang, Chen et al, 2020 ; Zhang, Shi et al, 2020 ).…”

Section: Introductionmentioning

confidence: 99%

“…The detection technologies of metabonomics mainly include gas chromatography-mass spectrometry (GC-MS), ultraperformance liquid chromatography/quadrupole time-of-flight mass spectrometry/mass spectrometry (UPLC-Q/TOF-MS/MS) and nuclear magnetic resonance. Among these detection technologies, UPLC-Q/TOF-MS/MS has the advantages of high sensitivity and high resolution (Wan et al, 2020;Zhang, Shi et al, 2020). UPLC-Q/TOF-MS/MS-based metabonomics, transcriptomics technology and enzyme-linked immunosorbent assay (ELISA) were used in this study to explore the liver injury mechanism in layer chickens caused by CS combined with LPS and analyze the differences in the intervention mechanisms of UASP and CASP.…”

Section: Introductionmentioning

confidence: 99%

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Metabonomics study on the hepatoprotective effect mechanism of polysaccharides from different processed products of Angelica sinensis on layer chickens based on UPLC–Q/TOF–MS/MS, multivariate statistical analysis and conjoint analysis

et al. 2022

Biomedical Chromatography

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Chicken colibacillosis is one of the most severe diseases in the poultry industry.Ceftiofur sodium (CS) is often used to treat it in clinical practice and lipopolysaccharide (LPS) accumulates in the chicken's body. Previous experimental studies found that CS combined with LPS could induce liver injury in layer chickens, and polysaccharides from charred Angelica sinensis(CASP) had a better hepatoprotective effect than polysaccharides from unprocessed Angelica sinensis(UASP). However, the intervention mechanism was unclear. Thus, UPLC-Q/TOF-MS/MS-based metabonomics and transcriptomics were used in this study to clarify the hepatoprotective effect mechanism of CASP and UASP in layer chickens. Transcriptomics and enzyme-linked immunosorbent assay were used for biological verification of some critical mutual metabolic pathways screened with metabonomics. The comprehensive analysis results showed that in a layer chicken liver injury model built with LPS and CS, 12 critical metabolic pathways were disturbed, involving 10 important differential metabolites. The hepatoprotective effect mechanism of CASP is related to the arachidonic acid metabolism and mTOR signaling pathways, involving nine important differential metabolites. In contrast, the hepatoprotective effect mechanism of UASP is related to the arachidonic acid metabolism pathway, involving six important differential metabolites.layer chickens, liver injury, LPS, metabonomics, polysaccharides from charred Angelica sinensis (CASP), polysaccharides from unprocessed Angelica sinensis(UASP)Abbreviations: ALT, alanine aminotransferase; AS, Angelica sinensis; AST, aspartate aminotransferase; CASP, polysaccharides from charred Angelica sinensis; CC, chicken colibacillosis; CS, ceftiofur sodium; FC, fold change; LPS, lipopolysaccharide; PLS-DA, partial least squares-discriminant analysis; UASP, polysaccharides from unprocessed AS; UPLC-Q/TOF-MS/MS, ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry/mass spectrometry; VIP, variable importance in projection; VN, Venn.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Metabonomics study on the hepatoprotective effect mechanism of polysaccharides from different processed products of Angelica sinensis on layer chickens based on UPLC–Q/TOF–MS/MS, multivariate statistical analysis and conjoint analysis

et al. 2022

Biomedical Chromatography

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“…Notably, we also observed substantial separation between prediabetes and T2DM patient samples in these PLS-DA plots, suggesting that prediabetic and diabetic individuals also exhibit distinct lipid metabolic profiled. R 2 Y represents the goodness of fit of the PLS-DA model on the Yaxis, while Q 2 estimates predictive capability (60). The R 2 Yand Q 2 of the established PLS-D model were 0.925 and 0.609.…”

Section: Exploration Of Distinct Lipidomic Profiles Associated With P...mentioning

confidence: 99%

Integrated lipids biomarker of the prediabetes and type 2 diabetes mellitus Chinese patients

Yang

Wang²,

Yang³

et al. 2023

Front. Endocrinol.

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IntroductionDyslipidemia is a hallmark of T2DM, and as such, analyses of lipid metabolic profiles in affected patients have the potential to permit the development of an integrated lipid metabolite-based biomarker model that can facilitate early patient diagnosis and treatment.MethodsUntargeted and targeted lipidomics approaches were used to analyze serum samples from newly diagnosed 93 Chinese participants in discovery cohort and 440 in validation cohort via UHPLC-MS and UHPLC-MS/MS first. The acid sphingomyelinase protein expression was analyzed by Western blot.Results and DiscussionThrough these analyses, we developed a novel integrated biomarker signature composed of LPC 22:6, PC(16:0/20:4), PE(22:6/16:0), Cer(d18:1/24:0)/SM(d18:1/19:0), Cer(d18:1/24:0)/SM(d18:0/16:0), TG(18:1/18:2/18:2), TG(16:0/16:0/20:3), and TG(18:0/16:0/18:2). The area under the curve (AUC) values for this integrated biomarker signature for prediabetes and T2DM patients were 0.841 (cutoff: 0.565) and 0.894 (cutoff: 0.633), respectively. Furthermore, theresults of western blot analysis of frozen adipose tissue from 3 week (prediabetes) and 12 week (T2DM) Goto–Kakizaki (GK) rats also confirmed that acid sphingomyelinase is responsible for significant disruptions in ceramide and sphingomyelin homeostasis. Network analyses of the biomarkers associated with this biosignature suggested that the most profoundly affected lipid metabolism pathways in the context of diabetes include de novo ceramide synthesis, sphingomyelin metabolism, and additional pathways associated with phosphatidylcholine synthesis. Together, these results offer new biological insights regarding the role of serum lipids in the context of insidious T2DM development, and may offer new avenues for future diagnostic and/or therapeutic research.

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Differences in the chemical composition of Panax ginseng roots infected with red rust

Guan

Fan

Sun

et al. 2022

Journal of Ethnopharmacology

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Plasma and urine metabonomics coupled with UPLC-Q-TOF/MS and multivariate data analysis on potential biomarkers in anemia and hematinic effects of steamed notoginseng

Cited by 3 publications

References 24 publications

Metabonomics study on the hepatoprotective effect mechanism of polysaccharides from different processed products of Angelica sinensis on layer chickens based on UPLC–Q/TOF–MS/MS, multivariate statistical analysis and conjoint analysis

Metabonomics study on the hepatoprotective effect mechanism of polysaccharides from different processed products of Angelica sinensis on layer chickens based on UPLC–Q/TOF–MS/MS, multivariate statistical analysis and conjoint analysis

Integrated lipids biomarker of the prediabetes and type 2 diabetes mellitus Chinese patients

Differences in the chemical composition of Panax ginseng roots infected with red rust

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