Poly-ε-caprolactone/hydroxyapatite for tissue engineering scaffold fabrication via selective laser sintering

Wiria, Florencia Edith; Leong, Kah Fai; Chua, Chee Kai; Liu, Y.

doi:10.1016/j.actbio.2006.07.008

Cited by 368 publications

(235 citation statements)

References 27 publications

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“…The FTIR spectra (not shown here) for uncrosslinked PPF and pristine HA nanoparticles have strong absorption peaks at 3440 cm -1 to indicate hydroxyl groups; however, these peaks are not prominent in the crosslinked PPF and PPF/HA nanocomposites. The absorption peaks are similar in all the FTIR spectra with a significant increase in the intensity of the absorption peak at 1000 cm -1 as the composition of HA increases because this absorption peak is due to the phosphate group in HA [17]. The absorption peaks assigned to methylene (−CH 2 -) and ester carbonyl (-C=O) groups are present at 2950 and 1740 cm -1 , respectively.…”

Section: Structural Characterizations and Morphologymentioning

confidence: 52%

“…However, it is very brittle and cannot be applied to the load-bearing site directly [3][4][5]. To overcome these limitations, HA has been incorporated with natural biomacromolecules such as collagen [6][7][8] and gelatin [9,10], or synthetic polymers such as poly (α-hydroxyl acids) [11][12][13][14][15], poly (ε-caprolactone) (PCL) [16,17], polyamide [18], and polymethylmethacrylate (PMMA) [19] to prepare composites using a variety of methods including surface coating, grafting, direct mixing, and biomimetic precipitation [10,11,[20][21][22][23]. Particularly, polymer/HA nanocomposites have improved mechanical properties and enhanced cell attachment, spreading, and proliferation on their surfaces by adding nano-sized HA to modify the polymer's characteristics and/or strengthen the polymer matrix [24,25].…”

Section: Introductionmentioning

confidence: 99%

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Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites

et al. 2008

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A series of crosslinkable nanocomposites has been developed using hydroxyapatite (HA) nanoparticles and poly(propylene fumarate) (PPF). PPF/HA nanocomposites with four different weight fractions of HA nanoparticles have been characterized in terms of thermal and mechanical properties. To assess surface chemistry of crosslinked PPF/HA nanocomposites, their hydrophilicity and capability of adsorbing proteins have been determined using static contact angle measurement and MicroBCA protein assay kit after incubation with 10% fetal bovine serum (FBS), respectively. In vitro cell studies have been performed using MC3T3-E1 mouse pre-osteoblast cells to investigate the ability of PPF/HA nanocomposites to support cell attachment, spreading, and proliferation after 1, 4, and 7 days. By adding HA nanoparticles to PPF, the mechanical properties of crosslinked PPF/ HA nanocomposites have not been increased due to the initially high modulus of crosslinked PPF. However, hydrophilicity and serum protein adsorption on the surface of nanocomposites have been significantly increased, resulting in enhanced cell attachment, spreading, and proliferation after 4 days of cell seeding. These results indicate that crosslinkable PPF/HA nanocomposites are useful for hard tissue replacement because of excellent mechanical strength and osteoconductivity.

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Section: Structural Characterizations and Morphologymentioning

confidence: 52%

Section: Introductionmentioning

confidence: 99%

Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites

et al. 2008

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“…A major limitation of the materials holding the most promise for tissue engineering is their suitability for laser sintering. Polycaprolactone (PCL) is investigated most extensively for scaffolds fabricated via laser sintering [2][3][4][5][6]. Other materials include poly(methyl methacrylate) (PMMA), polyetheretherketone (PEEK) [7], polylactide acid PLA [8] and poly(lactic-co-glycolide) (PLG) [9].…”

Section: Laser Sintering For Tissue Engineering Applicationsmentioning

confidence: 99%

“…Hydroxyapatide (HA), for instance, is known for its bioactive behaviour and osteoconductivity. HA has been blended with PCL, PE, PEEK, and polyvinylalcohol (PVA), and the compound powders have been processed via laser sintering [6,7,13,14].…”

Section: Compositesmentioning

confidence: 99%

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Laser Sintering for the Fabrication of Tissue Engineering Scaffolds

Lohfeld

McHugh

2012

Methods in Molecular Biology

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Tissue Engineering

Jagur‐Grodzinski¹

2008

Kirk-Othmer Encyclopedia of Chemical Technology

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The concept of tissue engineering (TE) was born when Vacanti and Langer suggested that living cells may form in vitro , at appropriate conditions, structures identical with those they form in live bodies. Tissue engineering is a fast growing branch of biomedical engineering. It currently consists of several branches. Seeding and culturing cells on scaffolds, which imitate extracellular matrix (ECM), is the basis of the most commonly used method. Scaffolds are prepared from various natural and synthetic polymers. Another variant of TE involves injection of gels that form scaffolds in situ . Acellular scaffolds may also be inserted in vivo . They mobilize cells from the adjacent tissues, from circulating body fluids, or stem cell sources. The above mentioned methods eliminate the in vitro stage of TE procedures. Another branch of TE is called cell‐sheet engineering (CSE). It involves culturing cell sheets on a surface of a polymer, whose structure undergoes sudden transition at a certain critical temperature ( T tr ). Cells are cultured at T > T tr . However, when a sheet is formed it separates spontaneously from the supporting surface when the temperature is decreased < T tr . Sheets thus formed can be harvested without damaging cell–cell or cell–ECM interactions. The CSE is particularly suitable for TE of liver and heart, since they consist of cells loosely associated with ECM. Materials used for formation of scaffolds and methods used for their preparation are described in some detail. Techniques, eg, rapid prototyping (RP) and photopattering, are discussed. Inverse RP, as well as the engineering of scaffolds surfaces, is also mentioned. The operation mechanism of various bioreactors for TE and their effect on the properties of the engineered tissues are reviewed.

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Poly-ε-caprolactone/hydroxyapatite for tissue engineering scaffold fabrication via selective laser sintering

Cited by 368 publications

References 27 publications

Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites

Physical properties and cellular responses to crosslinkable poly(propylene fumarate)/hydroxyapatite nanocomposites

Laser Sintering for the Fabrication of Tissue Engineering Scaffolds

Tissue Engineering

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