Polylactide Degradation by an Amycolatopsis sp

Pranamuda, Hardaning; Tokiwa, Yutaka; Tanaka, Hidehiko

doi:10.1128/aem.63.4.1637-1640.1997

Cited by 248 publications

(92 citation statements)

References 12 publications

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“…Some reports have been published concerning microbial degradation of PLLA. [1][2][3][4][5][6] We confirmed that the distribution of PLLA-degrading microorganisms is limited in the natural environment when compared with microorganisms that are able to degrade other polyesters, such as PCL and poly (b-hydroxybutyrate) (PHB). [1 -3] An Amycolatopsis sp.…”

Section: Introductionmentioning

confidence: 74%

“…strain HT-32 was successfully isolated and used to demonstrate PLLA-film degradation. [1] Further isolation of PLLA-degrading microorganisms led to the isolation of four actinomycetes and four bacteria; one actinomycete was identified as Amycolatopsis sp. (strain 41) on the basis of morphological observation and analysis of 16s RNA (H. Jikuya, pers.…”

Section: Introductionmentioning

confidence: 99%

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Poly (L-lactide)-Degrading Enzyme Produced by Amycolatopsis sp.

Pranamuda¹,

Tsuchii²,

Tokiwa³

2001

Macromol. Biosci.

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Section: Introductionmentioning

confidence: 74%

Section: Introductionmentioning

confidence: 99%

Poly (L-lactide)-Degrading Enzyme Produced by Amycolatopsis sp.

Pranamuda¹,

Tsuchii²,

Tokiwa³

2001

Macromol. Biosci.

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“…Compared with its synthesis, relatively little has been known for the biodegradation of PLA. Although it has been confirmed that PLA is naturally degraded in soil or compost and microorganisms able to degrade PLA have been described in many reports, PLA is known to be less susceptible to degradation in the natural environment than other aliphatic polyesters such as poly (b-hydroxybutyrate) (PHB) and poly (e-caprolactone) (PCL) (Pranamuda et al, 1995(Pranamuda et al, , 1997. On the other hand, PLA has been considered to be mainly degraded by proteinase-like enzymes with proteinase K being recognized as a typical PLA-degrading enzyme (Williams, 1981).…”

Section: Introductionmentioning

confidence: 99%

Purification and characterization of poly(l-lactic acid)-degrading enzymes fromAmycolatopsis orientalisssp.orientalis

Wang

Liu

et al. 2008

FEMS Microbiology Letters

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Polylactide or poly(l-lactic acid) (PLA) is a commercially promising material for use as a renewable and biodegradable plastic. Three novel PLA-degrading enzymes, named PLAase I, II and III, were purified to homogeneity from the culture supernatant of an effective PLA-degrading bacterium, Amycolatopsis orientalis ssp. orientalis. The molecular masses of these three PLAases as determined by sodium dodecyl sulfate-polyacrylamide gel electrophoresis were 24.0, 19.5 and 18.0 kDa, with the pH optima being 9.5, 10.5 and 9.5, respectively. The optimal temperature for the enzyme activities was 50-60 degrees C. All the purified enzymes could degrade high-molecular-weight PLA film as well as casein, and the PLA-degrading activities were strongly inhibited by serine protease inhibitors such as phenylmethylsulfonyl fluoride and aprotinin, but were not susceptive to chymostatin and pepstatin. Taken together, these data demonstrated that A. orientalis ssp. orientalis produces multiple serine-like proteases to utilize extracellular polylactide as a sole carbon source.

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“…PLA undergoes slow biodegradation in soil as compared to other biodegradable polyesters, because PLA is resistant to microbial attack and because of the limited distribution of PLA-degrading microorganisms in the soil. 55,56 The biodegradation of PLA films in the soil casting method was described in detail previously. 29 When buried in soil, polylactide film starts to decompose (lose weight and disintegrate) after approximately six months of incubation in soil.…”

Section: Surface Erosionmentioning

confidence: 99%

Environmental usefulness of PLA/PEG blends for controlled‐release systems of soil‐applied herbicides

Rychter

Lewicka

Rogacz

2019

J of Applied Polymer Sci

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Blends consisting of biodegradable polylactide (PLA) and poly(ethylene glycol) (PEG) were investigated for their usefulness as an environmentally friendly herbicide formulation with prolonged activity. The aim of this study was to evaluate the release rate of selected soil‐applied herbicides from the PLA/PEG blend containing PEG of various molecular weights and to assess the phytotoxicity of the PEGs according to OECD 208 guidelines. The release rate of immobilized herbicides was correlated with degradation of the blends used. The progress of PLA/PEG blend degradation in water, soil, and activated sludge was estimated by sample weight loss, changes in blend composition, and microscopic observations of the blend surfaces during the experiment. The proposed formulation of the immobilized herbicide in a blend consisting of slowly biodegradable PLA and water‐soluble PEG provides the possibility to release the herbicides for a relatively long time, for approximately six months, which is a demand of weed management. The effect of PEGs on plant growth and development was dependent on both their concentration and molecular weight. With a higher concentration in soil and a higher molecular weight of PEG, a more harmful effect on plants was noticed. © 2019 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019, 136, 47856.

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Polylactide Degradation by an Amycolatopsis sp

Cited by 248 publications

References 12 publications

Poly (L-lactide)-Degrading Enzyme Produced by Amycolatopsis sp.

Poly (L-lactide)-Degrading Enzyme Produced by Amycolatopsis sp.

Purification and characterization of poly(l-lactic acid)-degrading enzymes fromAmycolatopsis orientalisssp.orientalis

Environmental usefulness of PLA/PEG blends for controlled‐release systems of soil‐applied herbicides

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