Polymerase Chain Reaction Detection of &lt;em&gt;C. pseudotuberculosis&lt;/em&gt; in the Brain of  Mice Following Oral Inoculation

Jesse, Faez Firdaus Abdullah; Adamu, Lawan; Osman, Abdinasir Yusuf; Muhdi, Ahmad Fauzan Bin; Haron, Abd Wahid; Saharee, Abdul Aziz; Saad, Mohd Zamri; Omar, Abdul Rahman

doi:10.19026/ijava.5.5571

Cited by 9 publications

(13 citation statements)

References 19 publications

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“…The diagnosis of the suspected CLA was confirmed by laboratory diagnoses, the data revealed that all lymph nodes were positive for C. pseudotuberculosis because the primary target site for C. pseudotuberculosis replication is lymph nodes (27). Similar findings have been reported previously in sheep and goats using PCR detection of the bacteria from the lymph nodes by (28) and from lymph nodes of mice by (29). Based on standard microbiology methods including colony characteristics, Gram staining and biochemical tests all isolates showed the characteristics of C. pseudotuberculosis as mentioned by (30,31).…”

Section: Discussionsupporting

confidence: 84%

Prevalence and molecular investigation of caseous lymphadenitis among the slaughtered sheep at Duhok Abattoirs; experimental infection with Corynebacterium pseudotuberculosis in rabbits

Issa¹,

Fatah²,

Taha³

et al. 2021

IJVS

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Caseous lymphadenitis is a chronic infection caused by Corynebacterium pseudotuberculosis that affects the lymphatic system, resulting in abscesses in the lymph nodes and internal organs. This study was conducted to determine the prevalence rate of caseous lymphadenitis among slaughtered sheep in Duhok abattoirs, Duhok, Iraq and to investigate to what extend rabbits can develop caseous lymphadenitis just like that in sheep. A total of 1052 carcasses of sheep were randomly selected (939 males and 113 females) and carefully inspected for detection any suspected caseous lymph adenitis. The isolated Corynebacterium pseudotuberculosis from lymph nodes of sheep carcasses were identified by PCR before used in in vivo infection in rabbits. Data revealed that the prevalence rate of caseous lymphadenitis was at 1.9% among the slaughtered sheep and the prevalence rates were higher in females and older ages than that of males and younger ages, respectively. Experimentally infected rabbits developed various clinical signs were ranging from in inappetence to sudden death, mainly during first week of infection. Histopathologically, the infected rabbits developed caseous lymphadenitis and pyogranuloma after a time point of 10 days post-infection. Liver, spleen and lymph nodes of infected rabbits showed caseonecrotic foci with multiple micro-granulomas with presence of thick pyogenic membrane infiltrated with leukocytic cells; these findings are strictly resemblance to that reported in sheep. The obtained preliminary data of using rabbits as an animal model is promising which will enhance a better understanding the pathogenesis of caseous lymphadenitis in animals.

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Section: Discussionsupporting

confidence: 84%

Prevalence and molecular investigation of caseous lymphadenitis among the slaughtered sheep at Duhok Abattoirs; experimental infection with Corynebacterium pseudotuberculosis in rabbits

Issa¹,

Fatah²,

Taha³

et al. 2021

IJVS

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“…C. pseudotuberculosis is the causative agent of CLA in ruminants [ 12 , 13 ]. Although the bacterium causes low mortality, but it may influences the fertility of the infected animal, which may lead to major economic losses [ 14 ].…”

Section: Discussionmentioning

confidence: 99%

“…Khuder et al . [ 12 ] stated that C. pseudotuberculosis and its exotoxin (phospholipase D) able to cause disruption in reproductive hormones in mice model. There is a paucity of information in isolation of this organism from the reproductive tract of female goats in chronic infection.…”

Section: Introductionmentioning

confidence: 99%

Isolation and detection of Corynebacterium pseudotuberculosis in the reproductive organs and associated lymph nodes of non-pregnant does experimentally inoculated through intradermal route in chronic form

Latif¹,

Abdullah²,

Othman³

et al. 2015

Vet World

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Aim:Corynebacterium pseudotuberculosis is the etiological agent of caseous lymphadenitis that affects sheep and goats. This study was designed to determine the presence of the causative organism in the female reproductive organs and associated lymph nodes in non-pregnant does experimentally inoculated through intradermal route in the chronic form.Materials and Methods:18 non-pregnant healthy Katjang does aged 2-year-old were divided randomly into two groups. The first and second group consists of nine non-pregnant does each and the two groups were subdivided into three subgroups. The first group was experimentally inoculated with 1 ml of 107cfu of live C. pseudotuberculosis through intradermal route, whereas the second group was inoculated with 1 ml phosphate buffer saline (pH 7) solution intradermally. The first group were further subdivided into three subgroups where, the first subgroup (B1) were kept for 30 days post-infection, second subgroup (B2) were kept for 60 days post-infection, and third subgroup (B3) were kept for 90 days. The second group was further subdivided into three subgroups (C1, C2, and C3) where they were kept for 39, 60, and 90 days post-infection, respectively.Results:From this study, there was successful isolation of C. pseudotuberculosis from the reproductive organs of the treatment group after 60 days post-infection. The subgroups (B1, C1, C2, and C3) did not show any presence of the causative organism in the reproductive organs. The second subgroup B2 and third subgroup B3 showed positive isolation of the causative organisms from the ovary, uterine horns, uterus, cervix, vagina, and inguinal lymph node of the experimental non-pregnant does.Conclusion:This study showed that chronic infection of C. pseudotuberculosis via intradermal route may cause effect toward the reproductive organs and may be able to influence the reproductive efficiency of the infected animals.

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“…This organism is gram positive, non-spore and facultative anaerobic rod shaped bacteria, several of which are pathogenic for man and animals. As long as the animal becomes infected with C. pseudotuberculosis , it survives and replicates within cells of the immune system that normally be the defense system against it and once the disease established, CLA is difficult to eradicate [1] , [2] , [3] .…”

Section: Introductionmentioning

confidence: 99%

Antibacterial effect of gold nanoparticles against Corynebacterium pseudotuberculosis

Mohamed

Fouad

Elshoky³

et al. 2017

International Journal of Veterinary Science and Medicine

173

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Polymerase Chain Reaction Detection of <em>C. pseudotuberculosis</em> in the Brain of Mice Following Oral Inoculation

Cited by 9 publications

References 19 publications

Prevalence and molecular investigation of caseous lymphadenitis among the slaughtered sheep at Duhok Abattoirs; experimental infection with Corynebacterium pseudotuberculosis in rabbits

Prevalence and molecular investigation of caseous lymphadenitis among the slaughtered sheep at Duhok Abattoirs; experimental infection with Corynebacterium pseudotuberculosis in rabbits

Isolation and detection of Corynebacterium pseudotuberculosis in the reproductive organs and associated lymph nodes of non-pregnant does experimentally inoculated through intradermal route in chronic form

Antibacterial effect of gold nanoparticles against Corynebacterium pseudotuberculosis

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