Polymorphism Analysis of the Coagulase Gene in Isolates of Methicillin-Resistant Staphylococcus aureus with AluI Restriction Sites

Anggraini, Anita Dwi; Koendhori, Eko Budi; Pramono, Hendro; Wahyono, Daniel Djoko

doi:10.22435/hsji.v8i1.6865.

Cited by 2 publications

(2 citation statements)

References 0 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…11 Colony growth results after 24 hours which were round, convex, smooth-surfaced, and whitish with tender concentration were then selected. 12 A Gram staining examination was performed to determine the colony cell morphology of S. aureus which had a Gram positive cocci in grape-like clusters; therefore, confirmed coccus shaped with Gram (+). Catalase and coagulase test was conducted to determine the existence of S. aureus.…”

Section: Identification Of Saureusmentioning

confidence: 99%

The Examination of mecA gene in Methicillin-Resistant Staphylococcus aureus (MRSA) and inappropriate antibiotic uses of healthcare workers and communities in Banyumas

Susanti

Mahardhika

Rujito

et al. 2020

JKKI

View full text Add to dashboard Cite

Background: Methicillin-Resistant Staphylococcus aureus (MRSA) has become a major pathogene and its incidence continues to increase in various countries. The resistance to methicillin is caused by the mecA gene that encodes the expression of Penicillin Binding Protein 2a (PBP2a) so it has a low affinity for beta-lactam antibiotics. One of the factors that can increase the incidence of MRSA is inappropriate antibiotics use. Objective: This study is to identify presence of the mecA gene in MRSA and to compare histories of inappropriate antibiotic uses of healthcare workers and communities in Banyumas. Methods: This was a cross-sectional study conducting 120 samples from nasal swabs. Isolates of MRSA tested by bacteriological examinations and PCR of the mecA genes. Histories of antibiotic uses were assessed by using questionnaires and then analysed descriptively by using Fisher Exact test (SPPS, version 20). Result: One sample of S. aureus from the microbiology examination (0.83%) was an MRSA although there was no a mecA gene identified by using the PCR method. 66.1% of the healthcare workers and 88,3% of the communities used antibiotics inappropriately. The results showed that there was a statistically significant difference in the histories of irrational uses of antibiotics (p˂0.05). Conclusion:A number of the communities who used inappropriate antibiotics was higher than the health workers. Furthermore, one MRSA strain with a negative mecA gene was found in the health worker group. Latar Belakang: Methicillin-Resistant Staphylococcus aureus (MRSA) telah menjadi patogen utama dan angka insidensinya terus meningkat di berbagai negara. Resistensi terhadap methiciline disebabkan karena S.aureus memiliki gen mecA yang menyandi ekspresi dari Penicillin Binding Protein 2a (PBP2a) sehingga memiliki afinitas yang rendah terhadap antibiotik beta-laktam. Salah satu faktor yang dapat meningkatkan insidensi MRSA adalah penggunaan antibiotik tidak rasional Tujuan Penelitian: Tujuan penelitian ini adalah untuk mengetahui keberadaan gen mecA pada MRSA dan membandingkan riwayat penggunaan antibiotik pada tenaga kesehatan dan masyarakat di Banyumas.

show abstract

Section: Identification Of Saureusmentioning

confidence: 99%

The Examination of mecA gene in Methicillin-Resistant Staphylococcus aureus (MRSA) and inappropriate antibiotic uses of healthcare workers and communities in Banyumas

Susanti

Mahardhika

Rujito

et al. 2020

JKKI

View full text Add to dashboard Cite

show abstract

“…Restriction Fragment Length Polymorphism (RFLP), on the other hand, is a method used to analyze DNA sequences by cutting them into fragments using specific restriction enzymes. This technique facilitates the identification of gene polymorphisms (Anggraini et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Association of Growth Hormone Gen with KUB Chicken Productivity

Alfano,

Depison,

Erina

2023

BuletinPeternak

View full text Add to dashboard Cite

This study aims to investigate the diversity of quantitative characteristics and GH genes, along with the association between GH genes and quantitative characteristics. The research material consisted of 96 KUB chickens aged DOC-2 months and corresponding blood samples. The t-test was utilized to determine differences in body weight, weight gain, and body measurements between male and female KUB chickens, and assess the diversity of the GH gene. T2-Hotelling analysis was employed to compare body measurements between male and female KUB chickens, while principal component analysis was used to identify size and shape characteristics. Male KUB chickens' average body weight, weight gain and body size were significantly (p<0.05) higher than females. The analysis of the growth hormone gene exhibited three genotypes: +/+ (0.51), +/- (0.35), and -/- (0.14). Additionally, two alleles were identified: (+) accounting for 0.68% and (-) accounting for 0.32%. The growth hormone gene MspI of KUB chickens demonstrated polymorphism, with X2 count (2.93) < X2 table 0.05 (3.84). The heterozygosity value in the KUB chicken population was 0.43, and the obtained PIC value was 0.38. The genotype +/+ of the growth hormone gene in KUB chickens was significantly higher (p<0.05) than genotypes +/- and -/-.In conclusion, male KUB chickens exhibited higher body weight, weight gain, and body measurements compared to female KUB chickens. The chest circumference served as the identifier for body size in both male and female KUB chickens, while the length of the upper body and tibia length distinguished the shape characteristics of male and female KUB chickens, respectively. KUB chickens' growth hormone gene MspI was associated with body weight, weight gain, and body measurements, with the (+/+) genotype being the most favorable

show abstract

Polymorphism Analysis of the Coagulase Gene in Isolates of Methicillin-Resistant Staphylococcus aureus with AluI Restriction Sites

Cited by 2 publications

References 0 publications

The Examination of mecA gene in Methicillin-Resistant Staphylococcus aureus (MRSA) and inappropriate antibiotic uses of healthcare workers and communities in Banyumas

The Examination of mecA gene in Methicillin-Resistant Staphylococcus aureus (MRSA) and inappropriate antibiotic uses of healthcare workers and communities in Banyumas

Association of Growth Hormone Gen with KUB Chicken Productivity

Contact Info

Product

Resources

About