Polymorphisms of the IGF1 gene and their association with growth traits, serum concentration and expression rate of IGF1 and IGF1R in buffalo

Chau

et al. 2020

Cells

Forced expression of insulin-like growth factor binding proteins (IGFBPs) in transgenic mice has clearly revealed inhibitory effects on somatic growth. However, by this approach, it cannot be solved if or how IGFBPs rule insulin-like growth factor (IGF)-dependent growth under normal conditions. In order to address this question, we have used growth-selected mouse models (obese and lean) and studied IGF-1 and IGFBPs in serum with respect to longitudinal growth activity in males and females compared with unselected controls. In mice of both genders, body weights were recorded and daily weight gains were calculated. Between 2 and 54 weeks of age, serum IGF-1 was determined by ELISA and intact IGFBP-2, -3 and -4 were quantified by Western ligand blotting. The molar ratio of IGF-1 to the sum of IGFBP-2 to -4 was calculated for all groups and plotted against the daily weight gain curve. Growth-selected mice are characterized by higher daily weight gains and extended periods of elevated growth activity if compared to matched unselected controls. Therefore, adult mice from the obese and lean groups can achieve more than twofold increased body weight in both genders (p < 0.001). Between 2 and 11 weeks of age, in obese and lean mice of both genders, serum IGF-1 concentrations are increased more prominently if compared to unselected controls (p < 0.001). Instead, substantial decreases of IGFBPs, particularly of IGFBP-2, are observed in males and females of all groups at the age of 2 to 4 weeks (p < 0.001). Due to the strong increase of IGF-1 but not of IGFBPs between two and four weeks of age, the ratio of IGF-1 to IGFBP-2 to -4 in serum significantly increased in all groups and genders (p < 0.05). Notably, the IGF-1 to IGFBP ratio was higher in male and female obese mice if compared to unselected controls (p < 0.05).

Section: Introductionmentioning

confidence: 99%

Overlap of Peak Growth Activity and Peak IGF-1 to IGFBP Ratio: Delayed Increase of IGFBPs Versus IGF-1 in Serum as a Mechanism to Speed up and down Postnatal Weight Gain in Mice

Chau

et al. 2020

Cells

“…On contrary, Khederzadeh and Yazdanpanah (2013) observed three different SSCP patterns in amplified 265-bp of IGF-1 promoter region collected from five cities in Khuzestan province of Iran. Moreover, two novel single nucleotide polymorphisms (G64A and G280A) in the non-coding sequences of IGF-1 exon 1 and exon 4 of Egyptian buffaloes were detected by El-Magd et al (2017).…”

Section: Resultsmentioning

confidence: 98%

Polymorphism of IGF-1 and Its Receptor (IGF-1R) Genes and Their Association with Fertility Problems of Egyptian Buffaloes

Ramadan¹,

Abo-Gamil²,

El-Qaliouby³

2018

AJVS

The objectives of our study were to detect genetic polymorphisms in insulin-like growth factor-1 (IGF-1) and insulin-like growth factor1-receptor (IGF-1R) genes by using SCCP and direct sequence methods and to investigate their possible associations with fertility problems in Egyptian buffaloes. A total number of 100 buffalo heifers at Mahallet Mousa farm in Kafr El-sheikh province, Egypt were investigated. Animals were assigned into two main groups: normal fertile (50 animals) and infertile due to anestrous (50 animals). Blood samples were collected and the genomic DNA was extracted then PCR were performed with annealing temperatures at 58 o C and 55 o C for IGF1 and IGF1-R respectively. SSCP was performed for all amplified samples and loaded into a non-denaturing 12% polyacrylamide gel. Four amplicon from both of IGF-1 and IGF-1R genes for the two investigated groups were sequenced using the same primers. 265-bp from 5untranslated region (5-UTR) of IGF-1 gene and 311-bp from IGF-1R (part of intron 12 and part of exon 13) were amplified. In all investigated samples there was one SSCP pattern for IGF-1 and for IGF-1R genes in anestrus and normal fertile buffaloes' groups Moreover there were no polymorphisms neither in IGF-1 nor IGF-1R after the sequencing of four samples from each buffaloes' group for both of IGF-1 and IGF-1R genes. It seems that IGF-1 and IGF-1R are highly conserved in the investigated buffalo's population.

“…This study is part of a broader project targeting the genetic improvement of the of Egyptian water buffaloes. We previously screened IGF1, IGF1R, IGF2, IGF2R, and Cyp19A1 for polymorphisms and analyzed their associations with growth traits and fertility [15,16,18,24,26]. Herein, we extended our investigation to identify genetic variations in the GHR gene and determine their potential effects on milk production traits.…”

Section: Resultsmentioning

confidence: 99%

“…Again, Shi et al [13] did not study the effect of this important SNP on milk production and milk quality traits in buffaloes. Although many studies reported the ability of noncoding polymorphisms including: synonymous, intergenic and intronic SNPs to modify complex traits in animals [14][15][16], it is still essential to study non-synonymous SNPs, as they could directly alter the amino acids sequence, which could possibly lead to phenotypic variation. Therefore, in this study, we screened coding sequences of E4-E6 and E8 for polymorphisms.…”

Section: Introductionmentioning

confidence: 99%

Association of GHR Polymorphisms with Milk Production in Buffaloes

El-Komy

Saleh

Abdel-Hamid

et al. 2020

Animals

Self Cite

For its role in the mediation of growth hormone (GH) galactopoietic effect, growth hormone receptor (GHR) was considered a functional candidate gene for milk performance in cattle. However, its genetic variation and potential effect have not been investigated in Egyptian buffaloes. This study aimed to screen GHR for polymorphisms and study their associations with milk traits in Egyptian buffaloes. Polymerase chain reaction, single-strand conformation polymorphism, and sequencing were used to identify mutations in 4 exons (E4–E6 and E8) of the GHR gene in 400 Egyptian buffaloes. No polymorphisms were found in E4, while 2 SNPs (c.380G>A/p.Arg127Lys and c.387C>T/p.Gly129) in E5, one silent mutation (c.435A>G/p.Pro145) in E6, and another missense mutation (c.836T>A/p.Phe279Tyr) in E8 were detected. The c.380G>A SNP in the extracellular domain was associated with milk yield, fat %, protein %, and 305-day milk, fat and protein yield, with higher levels in animals carrying the mutant A allele. The c.836T>A SNP in the transmembrane domain was associated with milk yield, fat %, protein %, and 305-day milk, fat and protein yield, with higher milk yield and lower fat %, protein %, fat and protein yield in the mutant A allele-animals. Interestingly, animals with the two mutant AA alleles produced higher milk yield, fat %, protein %, fat and protein yield, accompanied with upregulated expressions of GHR, GH, insulin-like growth factor 1 (IGF1), prolactin (PRL), prolactin receptor (PRLR), β-casein (encoded by CSN2 gene), and diacylglycerol acyltransferase-1 (DGAT1) genes and proteins in milk somatic cells. Therefore, selection of Egyptian buffaloes with mutant AA haplotypes for the novel c.380G>A SNP and the well-known c.836T>A SNP could improve milk yield and quality in buffaloes.