Porous silicon based photoluminescence immunosensor for rapid and highly-sensitive detection of Ochratoxin A

Bovine leukaemia virus (BLV) proteins gp51, which are serving as antigens for specific antibodies against BLV proteins (anti-gp51), were applied as biological recognition part in the design of immunosensor devoted for the determination of anti-gp51. The efficiency of the immobilization of BLV proteins gp51 on ZnO nanorod (ZnO-NR) modified glass (ZnO-NR/glass) surface was evaluated. The formation of antigen-antibody complex on the ZnO/glass modified by the BLV proteins gp51 (gp51/ZnO-NR/glass) was investigated by the determination of changes in ZnO photoluminescence. The applicability of gp51/ZnO-NR/glass in the design of photoluminescence based immunosensor was evaluated. Bovine serum albumin (BSA) was applied for the modification of sensing gp51 layer in order to form gp51&BSA layer with advanced selectivity. Polyallylamine hydrochloride (PAH) was applied in order to improve the immobilization of gp51 and BSA based sensing layer (gp51&BSA) on the surface of ZnO-NR/glass. PAH was applied during the formation of gp51&BSA/PAH/ZnO-NR/glass structure. Some aspects of the mechanism of interaction between biomolecules (gp51, BSA and anti-gp51) and ZnO-NR during the preparation and action of gp51&BSA/ZnO-NR/glass-and gp51&BSA/PAH/ZnO-NR/glass-based immunosensors have been discussed.

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“…The response of developed immunosensors was calculated according to the following equation, which was reported earlier [18,35]:…”

Section: Testing Of Optical Immunosensor Based On Zno-nrsmentioning

confidence: 99%

Photoluminescence immunosensor based on bovine leukemia virus proteins immobilized on the ZnO nanorods

Viter

Savchuk

Стародуб

et al. 2019

Sensors and Actuators B: Chemical

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“…The proposed electrode definitely promise high potential for application in ultra low detection of analyzes. anti-OTA/Protein-A/PSi 0.001-100ng/mL 4.4pg mL -1 0.01-5ng/mL Myndrul et al [32] 11 Glass/ZnO-NRs/Protein-A/BSA/Anti-OTA 10-4-20ng mL -1 10 -2 ng mL -1 0.1-1ng/mL Viter et al [33] 12 aOTA/AuNPl/ ITO 1-20pg mL -1 0.6pg mL -1 2.89 × 10-5Apg mL -1 Present Table 2: A comparative report from the literature on the various electrochemical sensors of OTA.…”

Section: Resultsmentioning

confidence: 99%

Gold Nanopellets a Unique Platform for Electrochemical Ultra-Low Detection of Ochratoxin A

Basu¹

2018

FSN

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“…Functionalized-pSi immunosenor was tested under the wide range of concentrations (0.01–5 ng/ml) which was exhibited high sensitivity and high-speed detection even at low level of ochratoxin A. It was observed PL-intensity decreased as concentration of Ochratoxin A increased in sample (Myndrul et al, 2017 ). Carbon doped silicon nanoparticles were prepared by the mild reaction and used for detection Hg 2+ , Ag 2+ , and latent fingerprints.…”

Section: Applications Of Silicon-based Porous Nanomaterialsmentioning

confidence: 99%

Big Potential From Silicon-Based Porous Nanomaterials: In Field of Energy Storage and Sensors

et al. 2018

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Silicon nanoparticles (SiNPs) are the promising materials in the various applications due to their unique properties like large surface area, biocompatibility, stability, excellent optical and electrical properties. Surface, optical and electrical properties are highly dependent on particle size, doping of different materials and so on. Porous structures in silicon nanomaterials not only improve the specific surface area, adsorption, and photoluminescence efficiency but also provide numbers of voids as well as the high surface to volume ratio and enhance the adsorption ability. In this review, we focus on the significance of porous silicon/mesoporous silicon nanoparticles (pSiNPs/mSiNPs) in the applications of energy storage, sensors and bioscience. Silicon as anode material in the lithium-ion batteries (LIBs) faces a huge change in volume during charging/discharging which leads to cracking, electrical contact loss and unstable solid electrolyte interphase. To overcome challenges of Si anode in the LIBs, mSiNPs are the promising candidates with different structures and coating of different materials to enhance electrochemical properties. On the basis of optical properties with tunable wavelength, pSiNPs are catching good results in biosensors and gas sensors. The mSiNPs with different structures and modified surfaces are playing an important role in the detection of biomarkers, drug delivery and diagnosis of cancer and tumors.

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Porous silicon based photoluminescence immunosensor for rapid and highly-sensitive detection of Ochratoxin A

Cited by 66 publications

References 55 publications

Photoluminescence immunosensor based on bovine leukemia virus proteins immobilized on the ZnO nanorods

Photoluminescence immunosensor based on bovine leukemia virus proteins immobilized on the ZnO nanorods

Gold Nanopellets a Unique Platform for Electrochemical Ultra-Low Detection of Ochratoxin A

Big Potential From Silicon-Based Porous Nanomaterials: In Field of Energy Storage and Sensors

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