Possible precursors of regenerated hair cells in the avian cochlea following acoustic trauma

Girod, Douglas A.; Duckert, Larry G.; Rubel, Edwin W.

doi:10.1016/0378-5955(89)90143-3

Cited by 140 publications

(101 citation statements)

References 13 publications

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“…Although support cells are the most likely candidate (Girod et al, 1989;Raphael, 1992;Stone and Cotanche, 1994;Tsue et al, 1994a), only a small subset (ϳ15%) of support cells completes the cell cycle after elimination of hair cells in a region of the basilar papilla (Roberson et al, 1996). It is unclear in those experiments whether only a subset of support cells is capable of dividing or is stimulated to divide.…”

Section: Progenitor Cells In Culturesmentioning

confidence: 99%

“…Some animals demonstrate the capacity to generate hair cells throughout their lifetime (Popper and Hoxter, 1984;Corwin, 1985;Jörgenson and Mathiessen, 1989;Roberson et al, 1992) or to initiate hair cell regeneration in the event of their loss (Corwin and Cotanche, 1988;Ryals and Rubel, 1988). The progenitors of hair cells seem to be a subset of support cells that reside adjacent to hair cells in the sensory epithelia (Girod et al, 1989;Balak et al, 1990;Raphael, 1992;Hashino and Salvi, 1993;Weisleder and Rubel, 1993;Stone and Cotanche, 1994;Tsue et al, 1994a;Roberson et al, 1996). Although mature mammals normally do not generate new hair cells, recent in vivo and in vitro studies have documented mitotic activity and immature-looking hair cells in mammalian vestibular epithelia after exposure to ototoxic drugs Warchol et al, 1993;Rubel et al, 1995), suggesting that hair cell regeneration in mammals may be inducible.…”

Section: Abstract: Hair Cells; Regeneration; Chick; Basilar Papilla;mentioning

confidence: 99%

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Hair Cell Differentiation in Chick Cochlear Epithelium after Aminoglycoside Toxicity:In VivoandIn VitroObservations

Leaño²,

et al. 1996

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Inner ear epithelia of mature birds regenerate hair cells after ototoxic or acoustic insult. The lack of markers that selectively label cells in regenerating epithelia and of culture systems composed primarily of progenitor cells has hampered the identification of cellular and molecular interactions that regulate hair cell regeneration. In control basilar papillae, we identified two markers that selectively label hair cells (calmodulin and TUJ1 ␤ tubulin antibodies) and one marker unique for support cells (cytokeratin antibodies). Examination of regenerating epithelia demonstrated that calmodulin and ␤ tubulin are also expressed in early differentiating hair cells, and cytokeratins are retained in proliferative support cells. Enzymatic and mechanical methods were used to isolate sensory epithelia from mature chick basilar papillae, and epithelia were cultured in different conditions. In control cultures, hair cells are morphologically stable for up to 6 d, because calmodulin immunoreactivity and phalloidin labeling of filamentous actin are retained. The addition of an ototoxic antibiotic to cultures, however, causes complete hair cell loss by 2 d in vitro and generates cultures composed of calmodulinnegative, cytokeratin-positive support cells. These cells are highly proliferative for the first 2-7 d after plating, but stop dividing by 9 d. Calmodulin-or TUJ1-positive cells reemerge in cultures treated with antibiotic for 5 d and maintained for an additional 5 d without antibiotic. A subset of calmodulinpositive cells was also labeled with BrdU when it was continuously present in cultures, suggesting that some cells generated in culture begin to differentiate into hair cells. Key words: hair cells; regeneration; chick; basilar papilla; cell culture; differentiationHair cells are sensory receptors for hearing, equilibrium, and motion detection. Some animals demonstrate the capacity to generate hair cells throughout their lifetime (Popper and Hoxter, 1984;Corwin, 1985; Jörgenson and Mathiessen, 1989;Roberson et al., 1992) or to initiate hair cell regeneration in the event of their loss (Corwin and Cotanche, 1988;Ryals and Rubel, 1988). The progenitors of hair cells seem to be a subset of support cells that reside adjacent to hair cells in the sensory epithelia (Girod et al., 1989;Balak et al., 1990;Raphael, 1992;Hashino and Salvi, 1993;Weisleder and Rubel, 1993;Stone and Cotanche, 1994;Tsue et al., 1994a;Roberson et al., 1996). Although mature mammals normally do not generate new hair cells, recent in vivo and in vitro studies have documented mitotic activity and immature-looking hair cells in mammalian vestibular epithelia after exposure to ototoxic drugs Warchol et al., 1993;Rubel et al., 1995), suggesting that hair cell regeneration in mammals may be inducible. The development of culture methods for mature cochlear and vestibular end organs has been initiated to identify molecules that regulate cell proliferation and differentiation in avian and mammalian hair cell epithelia. Co-culture experiments suggest that...

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Section: Progenitor Cells In Culturesmentioning

confidence: 99%

Section: Abstract: Hair Cells; Regeneration; Chick; Basilar Papilla;mentioning

confidence: 99%

Hair Cell Differentiation in Chick Cochlear Epithelium after Aminoglycoside Toxicity:In VivoandIn VitroObservations

Leaño²,

et al. 1996

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“…There have been a number of studies describing cochlear pathology in birds as a result of acoustic overstimulation (Cotanche, 1987;Cotanche et al, 1987;Cotanche and Corwin, 1991;Cousillas and Rebillard, 1988;Corwin and Cotanche, 1988;Girod et al, 1989;Saunders et al, 1992) and ototoxicity of antibiotic drugs (Hashino et al, 1992;Hashino et al, 1991;Girod et al, 1991;Pickles and Rouse, 1991). We can thus compare the pathologies found in the cochlea of the Waterslagers with those observed in other birds after insult to the cochlea.…”

Section: /Hearing Research 79 (1994) 123-136 133mentioning

confidence: 99%

Inner-ear abnormalities and their functional consequences in Belgian Waterslager canaries (Serinus canarius)

Gleich

Dooling²,

Manley

1994

Hearing Research

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Recent reports of elevated auditory thresholds in canaries of the Belgian Waterslager strain have shown thai this strain has an inherited auditory deficit in which absolute auditory thresholds at high frequencies (i.e. above 2.(1 kHz) are as much as 41) dB less sensitive than the thresholds of mixed-breed canaries and those of other strains. The measurement of CAP audiograms showed that the hearing deficit is already present at the level of the auditory nerve (Gleich and Dooling, 1992). Here we show gross abnormalities in the anatomy of the basilar papilla of Belgian Waterslager canaries at the level of the hair cell. The extent of these abnormalities was correlated with the amount of hearing deficit as measured behaviorally.

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“…It has been hypothesized that the death of hair cells triggers the proliferation of nearby supporting cells (Corwin and Cotanche, 1988;Girod et al, 1989;Raphael and Altschuler, 1992;Roberson et al, 1992;Hashino and Salvi, 1993;Stone and Cotanche, 1994;Warchol and Corwin, 1996). To investigate the relationship between ongoing cell death and the level of supporting cell proliferation in the chick vestibular organs, we inhibited hair cell death by treatment with BAF.…”

Section: Preventing Ongoing Cell Death Reduces Supporting Cell Prolifmentioning

confidence: 99%

Inhibition of Caspases Prevents Ototoxic and Ongoing Hair Cell Death

2002

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Sensory hair cells die after acoustic trauma or ototoxic insults, but the signal transduction pathways that mediate hair cell death are not known. Here we identify several important signaling events that regulate the death of vestibular hair cells. Chick utricles were cultured in media supplemented with the ototoxic antibiotic neomycin and selected pharmacological agents that influence signaling molecules in cell death pathways. Hair cells that were treated with neomycin exhibited classically defined apoptotic morphologies such as condensed nuclei and fragmented DNA. Inhibition of protein synthesis (via treatment with cycloheximide) increased hair cell survival after treatment with neomycin, suggesting that hair cell death requires de novo protein synthesis. Finally, the inhibition of caspases promoted hair cell survival after neomycin treatment.Sensory hair cells in avian vestibular organs also undergo continual cell death and replacement throughout mature life. It is unclear whether the loss of hair cells stimulates the proliferation of supporting cells or whether the production of new cells triggers the death of hair cells. We examined the effects of caspase inhibition on spontaneous hair cell death in the chick utricle. Caspase inhibitors reduced the amount of ongoing hair cell death and ongoing supporting cell proliferation in a dosedependent manner. In isolated sensory epithelia, however, caspase inhibitors did not affect supporting cell proliferation directly. Our data indicate that ongoing hair cell death stimulates supporting cell proliferation in the mature utricle.

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Possible precursors of regenerated hair cells in the avian cochlea following acoustic trauma

Cited by 140 publications

References 13 publications

Hair Cell Differentiation in Chick Cochlear Epithelium after Aminoglycoside Toxicity:In VivoandIn VitroObservations

Hair Cell Differentiation in Chick Cochlear Epithelium after Aminoglycoside Toxicity:In VivoandIn VitroObservations

Inner-ear abnormalities and their functional consequences in Belgian Waterslager canaries (Serinus canarius)

Inhibition of Caspases Prevents Ototoxic and Ongoing Hair Cell Death

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